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[177064_at]

Description Y55D5A.F /REP_DB=WormBase Gene ID /WP=CE22517 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAG23455.1
Cluster 6grp_high1_10_10 > 61      6grp_total > 61      4grp_total > 41      6grp_high1_sub > 04      6grp_total_sub > Hat      6grp_rule_based > S4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 71
1 2 3 4 range
A 35.2
(-3)
A 		81.2
(1.5)
A 		68.3
(0.3)
A 		95.9
(3)
A 		61
B 66
(1.2)
A 		56.8
(0.1)
A 		82.2
(3)
A 		29.3
(-3)
A 		53
C 24.7
(-3)
A 		71.4
(3)
A 		69.4
(2.7)
A 		66.6
(2.4)
A 		47
avr 42
(-3) 		69.8
(2.3) 		73.3
(3) 		63.9
(1.2) 		31

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 139
1 2 3 4 5 6 range
A 50.2
(-3)
A 		108.9
(0.3)
P 		157.2
(3)
P 		156.5
(3)
P 		115.9
(0.7)
P 		82.6
(-1.2)
A 		107
B 97.4
(0)
P 		102.2
(0.3)
P 		151
(3)
P 		142.9
(2.6)
P 		99.8
(0.2)
P 		42.3
(-3)
A 		109
C 84.1
(-1.6)
A 		95.3
(-1.1)
P 		133.8
(0.7)
P 		180.9
(3)
P 		109.8
(-0.4)
P 		55.4
(-3)
P 		126
avr 77.2
(-2) 		102.1
(-0.5) 		147.3
(2.2) 		160.1
(3) 		108.5
(-0.1) 		60.1
(-3) 		100
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 18
1 2 3 4 range
A 12.8
(-3)
A 		17.6
(2.6)
A 		17.9
(3)
A 		14
(-1.6)
A 		5
B 21.1
(3)
A 		14
(0.1)
A 		19
(2.2)
A 		6.2
(-3)
A 		15
C 8.2
(-3)
A 		20.5
(1.6)
A 		24.2
(3)
A 		11.2
(-1.9)
A 		16
avr 14
(-0.9) 		17.4
(1.2) 		20.4
(3) 		10.5
(-3) 		10

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 31
1 2 3 4 5 6 range
A 9.3
(-3)
A 		38.7
(2.8)
P 		34.2
(1.9)
P 		39.7
(3)
P 		35
(2.1)
P 		20.1
(-0.9)
A 		30
B 33.9
(1.4)
P 		34
(1.4)
P 		36.7
(2.1)
P 		37.9
(2.4)
P 		40.5
(3)
P 		15.8
(-3)
A 		25
C 31.2
(1.3)
A 		29.1
(0.6)
P 		34.1
(2.2)
P 		36.4
(3)
P 		35.5
(2.7)
P 		18.5
(-3)
P 		18
avr 24.8
(-1) 		33.9
(1.8) 		35
(2.1) 		38
(3) 		37
(2.7) 		18.1
(-3) 		20
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177064_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18954
Sequence Type Exemplar sequence
Representative Public ID Y55D5A.F NCBI
Target Description Y55D5A.F /REP_DB=WormBase Gene ID /WP=CE22517 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAG23455.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065246(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065246 NCBI Caenorhabditis elegans Y55D5A.3 (Y55D5A.3) mRNA, complete cds. 11/11 None
GENEFINDER00000010561 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:2969983:2975775:-1 11/11 None
Y55D5A.3 ENSEMBL cdna:known chromosome:CEL140:III:2970667:2975775:-1 gene:Y55D5A.3 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000010549 7/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:2970666-2975775(-) 82.21 100.0
Public Domain and Genome References
Gene Title
Ensembl Y55D5A.3
Entrez Gene 190304 Entrez gene
SwissProt Q9GUI1 EMBL-EBI
WormBase CE27498 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CHICKEN:GGA.12233.1.S1_AT N-acylsphingosine amidohydrolase (acid ceramidase)-like gga
HG-U95AV2:33382_AT N-acylsphingosine amidohydrolase (acid ceramidase)-like hs
HG-U133_PLUS_2:214765_S_AT N-acylsphingosine amidohydrolase (acid ceramidase)-like hs
HG-U133A_2:214765_S_AT N-acylsphingosine amidohydrolase (acid ceramidase)-like hs
HG-U133A:214765_S_AT N-acylsphingosine amidohydrolase (acid ceramidase)-like hs
HG-U133_PLUS_2:215178_X_AT N-acylsphingosine amidohydrolase (acid ceramidase)-like hs
HG-U133A_2:215178_X_AT N-acylsphingosine amidohydrolase (acid ceramidase)-like hs
HG-U133A:215178_X_AT N-acylsphingosine amidohydrolase (acid ceramidase)-like hs
HU35KSUBA:HUM_ALU_AT N-acylsphingosine amidohydrolase (acid ceramidase)-like hs
HU35KSUBB:HUM_ALU_AT N-acylsphingosine amidohydrolase (acid ceramidase)-like hs
HU35KSUBC:HUM_ALU_AT N-acylsphingosine amidohydrolase (acid ceramidase)-like hs
HU35KSUBD:HUM_ALU_AT N-acylsphingosine amidohydrolase (acid ceramidase)-like hs
HUGENEFL:HUM_ALU_AT N-acylsphingosine amidohydrolase (acid ceramidase)-like hs
HC-G110:HUM_ALU_AT N-acylsphingosine amidohydrolase (acid ceramidase)-like hs
HUGENEFL:M92449_AT N-acylsphingosine amidohydrolase (acid ceramidase)-like hs
U133_X3P:HS.264330.4.A1_3P_X_AT N-acylsphingosine amidohydrolase (acid ceramidase)-like hs
U133_X3P:HS.264330.3.S1_3P_X_AT N-acylsphingosine amidohydrolase (acid ceramidase)-like hs
U133_X3P:214765_3P_S_AT N-acylsphingosine amidohydrolase (acid ceramidase)-like hs
HG-U95E:67241_AT N-acylsphingosine amidohydrolase (acid ceramidase)-like hs
HU35KSUBB:RC_R09547_AT N-acylsphingosine amidohydrolase (acid ceramidase)-like hs
U133_X3P:HS.264330.2.A1_3P_AT N-acylsphingosine amidohydrolase (acid ceramidase)-like hs
U133_X3P:HS.16478.0.S1_3P_X_AT N-acylsphingosine amidohydrolase (acid ceramidase)-like hs
U133_X3P:HS.16478.0.S1_3P_AT N-acylsphingosine amidohydrolase (acid ceramidase)-like hs
U133_X3P:232072_3P_AT N-acylsphingosine amidohydrolase (acid ceramidase)-like hs
HG-U133_PLUS_2:227135_AT N-acylsphingosine amidohydrolase (acid ceramidase)-like hs
HG-U133B:227135_AT N-acylsphingosine amidohydrolase (acid ceramidase)-like hs
HG-U133_PLUS_2:232072_AT N-acylsphingosine amidohydrolase (acid ceramidase)-like hs
HG-U133B:232072_AT N-acylsphingosine amidohydrolase (acid ceramidase)-like hs
HG-U95C:56977_AT N-acylsphingosine amidohydrolase (acid ceramidase)-like hs
HG-U95B:44452_R_AT N-acylsphingosine amidohydrolase (acid ceramidase)-like hs
HG-U95E:84166_AT N-acylsphingosine amidohydrolase (acid ceramidase)-like hs
HU35KSUBB:RC_H62897_AT N-acylsphingosine amidohydrolase (acid ceramidase)-like hs
MG-U74BV2:109102_R_AT N-acylsphingosine amidohydrolase (acid ceramidase)-like mm
MG-U74BV2:164882_R_AT N-acylsphingosine amidohydrolase (acid ceramidase)-like mm
MG-U74CV2:169922_AT N-acylsphingosine amidohydrolase (acid ceramidase)-like mm
MOE430A:1452067_AT N-acylsphingosine amidohydrolase (acid ceramidase)-like mm
MOUSE430A_2:1452067_AT N-acylsphingosine amidohydrolase (acid ceramidase)-like mm
MOUSE430_2:1452067_AT N-acylsphingosine amidohydrolase (acid ceramidase)-like mm
MOE430A:1452068_AT N-acylsphingosine amidohydrolase (acid ceramidase)-like mm
MOUSE430A_2:1452068_AT N-acylsphingosine amidohydrolase (acid ceramidase)-like mm
MOUSE430_2:1452068_AT N-acylsphingosine amidohydrolase (acid ceramidase)-like mm
MOE430B:1430819_AT N-acylsphingosine amidohydrolase (acid ceramidase)-like mm
MOUSE430_2:1430819_AT N-acylsphingosine amidohydrolase (acid ceramidase)-like mm
MU19KSUBA:TC21299_AT N-acylsphingosine amidohydrolase (acid ceramidase)-like (Asahl), mRNA mm
Protein Similarities
Method ID Description E-Value
blast AAK39622 Hypothetical protein Y55D5A.3 [Caenorhabditis elegans] ref|NP_497647.1| Y55D5A.3 [Caenorhabditis elegans] 0.0
blast CAE57502 Hypothetical protein CBG00476 [Caenorhabditis briggsae] 1.0E-153
blast CAE57502 Hypothetical protein CBG00476 [Caenorhabditis briggsae] 1.0E-152
blast NP_001005714 N-acylsphingosine amidohydrolase (acid ceramidase)-like [Xenopus tropicalis] gb|AAH75301.1| N-acylsphingosine amidohydrolase (acid ceramidase)-like [Xenopus tropicalis] 8.0E-54
blast NP_001005714 N-acylsphingosine amidohydrolase (acid ceramidase)-like [Xenopus tropicalis] gb|AAH75301.1| N-acylsphingosine amidohydrolase (acid ceramidase)-like [Xenopus tropicalis] 3.0E-53
Protein Domains
Database ID Description E-Value
Pfam IPR003199 EMBL-EBI Choloylglycine hydrolase 1.7E-5
Sequence
Target Sequence 
>C. ELEGANS:177064_AT
gaaacgggaccatacagtactccggactcacatttgccctgtataatggagtacttacag
gacagcggcctggtgaatacagtgtcagcttgaatgcaaggtactctggcgcctacatcg
ataacattttaatggagttctacacgaaattcaagcgaccggtgagcttcttcattcggg
atgttctggagaatcaggcgacctatacagaagctgtggatgctttttcgagaacacatc
tgttttctccttcttatataattgtggctggaatcaagaaaaatgaaggagttgttattt
ccagaaaccgttggtcagctgcaaatgtgtaccctctaaacgtagacgccaatcaatggt
tcctcgtggaaaccaactttgataactggaaaaagcaaggcgatgaccgaagaatcactg
caattcagaagcttaaagagctcggaagacggaatttcgatgaaaagtcaatggttgaag
tgttgtcgactgtgcctgtcaggaataatttgaccgtttttagtactgtaatggtgccgg
gcttgccggattc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAACGGGACCATACAGTACTCCGG 665 355 1022 Antisense
GGACTCACATTTGCCCTGTATAATG 35 523 1045 Antisense
TGAATGCAAGGTACTCTGGCGCCTA 352 575 1112 Antisense
TGGCGCCTACATCGATAACATTTTA 146 543 1128 Antisense
TCAAGCGACCGGTGAGCTTCTTCAT 477 629 1172 Antisense
GAGCTTCTTCATTCGGGATGTTCTG 267 389 1185 Antisense
ATCTGTTTTCTCCTTCTTATATAAT 53 35 1259 Antisense
TCAGCTGCAAATGTGTACCCTCTAA 623 623 1336 Antisense
AGACGCCAATCAATGGTTCCTCGTG 589 69 1365 Antisense
TGTTGTCGACTGTGCCTGTCAGGAA 463 563 1502 Antisense
TAATGGTGCCGGGCTTGCCGGATTC 334 633 1550 Antisense
Sequence Source Affymetrix Proprietary Database