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[177055_at]

Description Y65B4BM.A /REP_DB=WormBase Gene ID /WP=CE25538 /CHR=1 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF60853.1
Cluster 6grp_total > 53      4grp_total > 49      6grp_total_sub > Hat      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 57
1 2 3 4 range
A 7.1
(-2.8)
A 		17.9
(-1.6)
A 		60.4
(3)
A 		4.8
(-3)
A 		56
B 9.7
(-2.2)
A 		39.5
(2)
A 		47
(3)
A 		4.1
(-3)
A 		43
C 6.2
(-2.7)
A 		58.7
(3)
A 		12.1
(-2.1)
A 		3.4
(-3)
A 		55
avr 7.7
(-2.4) 		38.7
(2.8) 		39.8
(3) 		4.1
(-3) 		36

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 29
1 2 3 4 5 6 range
A 17.9
(-0.5)
A 		6.5
(-3)
A 		34.4
(3)
A 		16.6
(-0.8)
A 		30.8
(2.2)
A 		8.7
(-2.5)
A 		28
B 10.6
(-3)
A 		15.5
(-1.2)
A 		27.1
(3)
A 		10.9
(-2.9)
A 		22.5
(1.3)
A 		13.3
(-2)
A 		17
C 7.2
(-1.5)
A 		10.5
(1)
A 		8
(-0.9)
A 		13.2
(3)
A 		12
(2.1)
A 		5.2
(-3)
A 		8
avr 11.9
(-1.8) 		10.8
(-2.3) 		23.2
(3) 		13.6
(-1.1) 		21.8
(2.4) 		9.1
(-3) 		14
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 16
1 2 3 4 range
A 2.6
(-2.2)
A 		3.9
(-1.7)
A 		15.8
(3)
A 		0.7
(-3)
A 		15
B 3.1
(-1.7)
A 		9.8
(2.3)
A 		10.9
(3)
A 		0.9
(-3)
A 		10
C 2.1
(-2.4)
A 		16.9
(3)
A 		4.2
(-1.7)
A 		0.6
(-3)
A 		16
avr 2.6
(-1.8) 		10.2
(2.9) 		10.3
(3) 		0.7
(-3) 		10

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 8
1 2 3 4 5 6 range
A 3.3
(-2)
A 		2.3
(-2.8)
A 		7.5
(1.5)
A 		4.2
(-1.3)
A 		9.3
(3)
A 		2.1
(-3)
A 		7
B 3.7
(-2.2)
A 		5.2
(-0.8)
A 		6.6
(0.6)
A 		2.9
(-3)
A 		9.1
(3)
A 		5
(-1)
A 		6
C 2.7
(-0.3)
A 		3.2
(1.1)
A 		2
(-2.2)
A 		2.6
(-0.5)
A 		3.9
(3)
A 		1.7
(-3)
A 		2
avr 3.2
(-2.6) 		3.6
(-2.1) 		5.4
(0.3) 		3.2
(-2.6) 		7.4
(3) 		2.9
(-3) 		5
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177055_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18866
Sequence Type Exemplar sequence
Representative Public ID Y65B4BM.A NCBI
Target Description Y65B4BM.A /REP_DB=WormBase Gene ID /WP=CE25538 /CHR=1 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF60853.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058345(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058345 NCBI Caenorhabditis elegans Y65B4BL.1 (Y65B4BL.1) mRNA, complete cds. 11/11 A 
SNAP00000040952 ENSEMBL cdna:SNAP chromosome:CEL140:I:531950:532855:1 11/11 None
GENEFINDER00000040953 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:531951:532855:1 11/11 None
Y65B4BL.1 ENSEMBL cdna:known chromosome:CEL140:I:530569:532855:1 gene:Y65B4BL.1 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:531950-532855(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y65B4BL.1
Entrez Gene 171644 Entrez gene
SwissProt Q9N300 EMBL-EBI
WormBase CE29898 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAF60849 Hypothetical protein Y65B4BL.1 [Caenorhabditis elegans] ref|NP_490746.2| Y65B4BL.1 [Caenorhabditis elegans] 0.0
blast CAE61288 Hypothetical protein CBG05111 [Caenorhabditis briggsae] 1.0E-164
blast AAF60849 Hypothetical protein Y65B4BL.1 [Caenorhabditis elegans] ref|NP_490746.2| Y65B4BL.1 [Caenorhabditis elegans] 1.0E-156
blast CAE61288 Hypothetical protein CBG05111 [Caenorhabditis briggsae] 1.0E-135
blast NP_508084 R57.2 [Caenorhabditis elegans] gb|AAB52661.3| Hypothetical protein R57.2 [Caenorhabditis elegans] 9.0E-33
Protein Domains
Database ID Description E-Value
Pfam IPR002602 EMBL-EBI Protein of unknown function DB 5.8E-26
Pfam IPR002602 EMBL-EBI Protein of unknown function DB 5.8E-26
Pfam IPR002602 EMBL-EBI Protein of unknown function DB 5.8E-26
Sequence
Target Sequence 
>C. ELEGANS:177055_AT
agcagttcatcgactccaaggatatcagaagagcaccacgagtcggtgatgccaagcttc
tcagccaggagtacccgatcaccgaggtcaccgacgccgatctctcctccgagtgcggaa
ccgagaagtctcagccaccattctcaccatgcttgtcccgcaagtcggccgacgacgtct
tcctctcctgctgtcgccaacaggttccatccaactgtcacagcctctgcacctacgagc
atcgtgagcatgtcgccgctgaaactctgatccaggcgatccagcaagagcactgcgata
tgaagtacttctccaaactgctctactgtgccaaccaaaaccgcgacaaccgcgcgtgct
gctccaacctcggcatgtcaaacgcggaactcggcgtcggcgatcgttgcctacgtatgt
gcaacatctccccgtcgggagaccgtgtaagctctatggagaaggaggatctcgtctgcc
tgtccaactggaacgtcat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGCAGTTCATCGACTCCAAGGATAT 114 77 314 Antisense
GAGCACCACGAGTCGGTGATGCCAA 423 389 344 Antisense
TCTCCTCCGAGTGCGGAACCGAGAA 10 609 416 Antisense
CTACGAGCATCGTGAGCATGTCGCC 15 227 546 Antisense
GAAACTCTGATCCAGGCGATCCAGC 11 357 574 Antisense
TGAAGTACTTCTCCAAACTGCTCTA 295 575 614 Antisense
TCTACTGTGCCAACCAAAACCGCGA 134 613 635 Antisense
CGGCGATCGTTGCCTACGTATGTGC 116 243 711 Antisense
ACATCTCCCCGTCGGGAGACCGTGT 634 103 737 Antisense
GAGAAGGAGGATCTCGTCTGCCTGT 596 391 772 Antisense
TCTGCCTGTCCAACTGGAACGTCAT 188 609 788 Antisense
Sequence Source Affymetrix Proprietary Database