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[177051_at]

Description Y73B6BL.Q /REP_DB=WormBase Gene ID /WP=CE26289 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAG23475.1
Cluster 4grp_high_8_8 > 13      6grp_total > 24      4grp_total > 28      6grp_cgc > 31      6grp_cgc_sub > De      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 75
1 2 3 4 range
A 59.3
(-0.4)
P 		67.5
(1)
P 		44.6
(-3)
A 		79
(3)
A 		34
B 82.7
(0.5)
P 		66
(-1.1)
P 		46.9
(-3)
A 		107.7
(3)
P 		61
C 59.9
(2)
P 		65
(3)
P 		60.3
(2.1)
P 		33.2
(-3)
A 		32
avr 67.3
(1.4) 		66.2
(1.1) 		50.6
(-3) 		73.3
(3) 		23

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 136
1 2 3 4 5 6 range
A 62.4
(0.8)
P 		72.9
(1.5)
P 		96.6
(3)
P 		58.6
(0.6)
P 		26.1
(-1.5)
A 		1.9
(-3)
A 		95
B 92.6
(2.6)
P 		96.8
(3)
P 		70.7
(0.7)
P 		28.2
(-3)
A 		58.6
(-0.3)
P 		30.7
(-2.8)
A 		69
C 138.3
(3)
P 		71
(-0.4)
P 		55.5
(-1.2)
P 		24.8
(-2.7)
A 		40.1
(-1.9)
P 		18.8
(-3)
A 		120
avr 97.8
(3) 		80.2
(1.7) 		74.3
(1.3) 		37.2
(-1.5) 		41.6
(-1.2) 		17.1
(-3) 		81
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 21
1 2 3 4 range
A 21.6
(3)
P 		14.7
(-1.1)
P 		11.7
(-2.9)
A 		11.6
(-3)
A 		10
B 26.4
(3)
P 		16.3
(-0.9)
P 		10.9
(-3)
A 		22.9
(1.6)
P 		16
C 19.8
(2.5)
P 		18.7
(2.1)
P 		21
(3)
P 		5.6
(-3)
A 		15
avr 22.6
(3) 		16.6
(-0.9) 		14.5
(-2.3) 		13.4
(-3) 		9

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 51
1 2 3 4 5 6 range
A 11.5
(-0.4)
P 		25.9
(3)
P 		21
(1.8)
P 		14.9
(0.4)
P 		7.9
(-1.3)
A 		0.5
(-3)
A 		25
B 32.2
(3)
P 		32.2
(3)
P 		17.2
(-0.6)
P 		7.5
(-3)
A 		23.8
(1)
P 		11.4
(-2.1)
A 		25
C 51.3
(3)
P 		21.7
(-0.8)
P 		14.1
(-1.8)
P 		5
(-3)
A 		13
(-2)
P 		6.3
(-2.8)
A 		46
avr 31.7
(3) 		26.6
(1.8) 		17.4
(-0.4) 		9.1
(-2.3) 		14.9
(-0.9) 		6.1
(-3) 		26
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177051_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19256
Sequence Type Exemplar sequence
Representative Public ID Y73B6BL.Q NCBI
Target Description Y73B6BL.Q /REP_DB=WormBase Gene ID /WP=CE26289 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAG23475.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068567(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068567 NCBI Caenorhabditis elegans MIF (macrophage migration inhibitory factor) related family member (mif-4) (mif-4) mRNA, complete cds. 11/11 None
SNAP00000032040 ENSEMBL cdna:SNAP chromosome:CEL140:IV:6335233:6335971:-1 10/11 None
GENEFINDER00000032076 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:6335072:6335971:-1 11/11 None
Y73B6BL.13 ENSEMBL cdna:known chromosome:CEL140:IV:6335233:6335971:-1 gene:Y73B6BL.13 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:6335238-6335977(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol mif-4
Ensembl Y73B6BL.13
Entrez Gene 190649 Entrez gene
SwissProt Q9GUG4 EMBL-EBI
WormBase CE26289 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAG23475 Mif (macrophage migration inhibitory factor) related protein 4 [Caenorhabditis elegans] ref|NP_500968.1| MIF (macrophage migration inhibitory factor) related family member (mif-4) [Caenorhabditis elegans] 2.0E-61
blast AAG23475 Mif (macrophage migration inhibitory factor) related protein 4 [Caenorhabditis elegans] ref|NP_500968.1| MIF (macrophage migration inhibitory factor) related family member (mif-4) [Caenorhabditis elegans] 1.0E-59
blast AAG23475 Mif (macrophage migration inhibitory factor) related protein 4 [Caenorhabditis elegans] ref|NP_500968.1| MIF (macrophage migration inhibitory factor) related family member (mif-4) [Caenorhabditis elegans] 9.0E-59
blast CAE61558 Hypothetical protein CBG05467 [Caenorhabditis briggsae] 2.0E-48
blast CAE61558 Hypothetical protein CBG05467 [Caenorhabditis briggsae] 1.0E-46
blast CAE61558 Hypothetical protein CBG05467 [Caenorhabditis briggsae] 2.0E-46
blast CAE61570 Hypothetical protein CBG05481 [Caenorhabditis briggsae] 7.0E-21
Sequence
Target Sequence 
>C. ELEGANS:177051_AT
gaattcaaaccaacatccgaagtgctgatattccagaaaagtttgaacaagatgtgatct
acaatttatcagttgtaatggaattaccagctgataaattcgtgattattgttgagccgg
cagttagaatgagaattggatttgagaataaagaaattccagttgcaattgtaaatttcc
aaacaacccggccttcttctcgaatcgaaaatgactcatatgcaaaaaaattgacatcag
tgctcaatgagcagttgaagcttgatcccgcccatattttcatatcatttg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAATTCAAACCAACATCCGAAGTGC 88 321 26 Antisense
AACATCCGAAGTGCTGATATTCCAG 354 135 37 Antisense
GATGTGATCTACAATTTATCAGTTG 407 409 76 Antisense
ATTACCAGCTGATAAATTCGTGATT 251 13 108 Antisense
ATTCGTGATTATTGTTGAGCCGGCA 124 9 123 Antisense
GTTGAGCCGGCAGTTAGAATGAGAA 85 439 136 Antisense
ATTCCAGTTGCAATTGTAAATTTCC 597 7 181 Antisense
TCTCGAATCGAAAATGACTCATATG 565 607 223 Antisense
TGCTCAATGAGCAGTTGAAGCTTGA 88 581 266 Antisense
TGATCCCGCCCATATTTTCATATCA 424 565 288 Antisense
CCCGCCCATATTTTCATATCATTTG 366 261 292 Antisense
Sequence Source Affymetrix Proprietary Database