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[177045_at]

Description Y55F3BR.F /REP_DB=WormBase Gene ID /WP=CE22539 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF59604.1
Cluster 6grp_total > 66      4grp_total > 52      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 269
1 2 3 4 range
A 410.5
(3)
P 		405.4
(2.9)
A 		370.1
(2.1)
A 		141.9
(-3)
A 		269
B 324.1
(2.7)
M 		331.8
(3)
A 		297.2
(1.9)
A 		150.8
(-3)
A 		181
C 377
(3)
P 		365.7
(2.5)
A 		324.2
(0.6)
A 		246.8
(-3)
A 		130
avr 370.5
(3) 		367.6
(2.9) 		330.5
(1.7) 		179.8
(-3) 		191

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 286
1 2 3 4 5 6 range
A 168.5
(-1.9)
A 		155
(-2.7)
A 		256.5
(3)
A 		149.6
(-3)
A 		184
(-1.1)
A 		242.3
(2.2)
A 		107
B 217.7
(-1.2)
A 		150.2
(-2.8)
A 		194.1
(-1.8)
A 		201.6
(-1.6)
A 		144
(-3)
A 		386.9
(3)
P 		243
C 120.3
(-3)
A 		157.5
(-2.2)
A 		180.5
(-1.7)
A 		184.3
(-1.7)
A 		120.8
(-3)
A 		406.5
(3)
P 		286
avr 168.8
(-2.4) 		154.2
(-2.9) 		210.4
(-1.1) 		178.5
(-2.1) 		149.6
(-3) 		345.2
(3) 		196
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 129
1 2 3 4 range
A 149.5
(3)
P 		88
(0.1)
A 		96.7
(0.5)
A 		20.8
(-3)
A 		129
B 103.3
(3)
M 		82
(1.2)
A 		68.9
(0.1)
A 		32.1
(-3)
A 		71
C 124.8
(3)
P 		105.1
(1.6)
A 		113
(2.1)
A 		41.6
(-3)
A 		83
avr 125.9
(3) 		91.7
(0.8) 		92.9
(0.9) 		31.5
(-3) 		94

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 113
1 2 3 4 5 6 range
A 31.1
(-3)
A 		55.1
(2.2)
A 		55.9
(2.3)
A 		37.9
(-1.5)
A 		55.5
(2.2)
A 		59
(3)
A 		28
B 75.7
(-1.2)
A 		50
(-2.8)
A 		47.1
(-3)
A 		53.4
(-2.6)
A 		58.5
(-2.3)
A 		144.3
(3)
P 		97
C 44.6
(-2.5)
A 		48.1
(-2.3)
A 		46
(-2.5)
A 		37.1
(-3)
A 		39.1
(-2.9)
A 		136.1
(3)
P 		99
avr 50.5
(-2.3) 		51.1
(-2.3) 		49.7
(-2.4) 		42.8
(-3) 		51
(-2.3) 		113.1
(3) 		70
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177045_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19194
Sequence Type Exemplar sequence
Representative Public ID Y55F3BR.F NCBI
Target Description Y55F3BR.F /REP_DB=WormBase Gene ID /WP=CE22539 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF59604.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067597(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067597 NCBI Caenorhabditis elegans Y55F3BR.6 (Y55F3BR.6) mRNA, complete cds. 10/11 None
GENEFINDER00000039577 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:835777:838560:-1 11/11 None
Y55F3BR.6 ENSEMBL cdna:known chromosome:CEL140:IV:835048:838560:-1 gene:Y55F3BR.6 10/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000039566 5/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:835782-838566(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y55F3BR.6
Entrez Gene 190317 Entrez gene
SwissProt Q9N350 EMBL-EBI
WormBase CE35111 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAF59604 Hypothetical protein Y55F3BR.6 [Caenorhabditis elegans] ref|NP_499998.2| Y55F3BR.6 [Caenorhabditis elegans] 1.0E-114
blast AAF59604 Hypothetical protein Y55F3BR.6 [Caenorhabditis elegans] ref|NP_499998.2| Y55F3BR.6 [Caenorhabditis elegans] 1.0E-108
blast CAE56258 Hypothetical protein CBG23899 [Caenorhabditis briggsae] 3.0E-99
blast CAE56258 Hypothetical protein CBG23899 [Caenorhabditis briggsae] 7.0E-93
blast AAQ62453 Heat shock protein protein 17, isoform b [Caenorhabditis elegans] ref|NP_001023958.1| Heat Shock Protein family member (hsp-17) [Caenorhabditis elegans] 3.0E-14
blast AAV36947 LP13637p [Drosophila melanogaster] gb|AAF50287.1| CG4167-PA [Drosophila melanogaster] ref|NP_523998.1| CG4167-PA [Drosophila melanogaster] 3.0E-10
Protein Domains
Database ID Description E-Value
Pfam IPR002068 EMBL-EBI Heat shock protein Hsp20 5.5E-17
Pfam IPR002068 EMBL-EBI Heat shock protein Hsp20 1.9E-13
Sequence
Target Sequence 
>C. ELEGANS:177045_AT
atcttgccgagatttctgaacgatttgaaaaattaagagcacggagcagcaataatatgg
ctacgttcaacactacaagctcctacaatcggacttatgagaaaaaggtcatcgaggaag
gatctccaaatgttcgtgtcaccacgcaaactcatactgcaattccaggaatgccggtaa
ccgtcgtcgataacaacataatcgttgaaggatcgcatggagaaaaggaagatacctatg
gaaccatcgaatcaaccttcaaacgtcgctttccacttccaaaagccgtcgcaccggaat
ctgttcaatctcagctcactgctgatggtcatttgactattgatgcaaaagcaccggagc
cgaaacaagaaggagctcgtccgattcagatta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATCTTGCCGAGATTTCTGAACGATT 87 31 47 Antisense
CTACGTTCAACACTACAAGCTCCTA 438 223 107 Antisense
ACAAGCTCCTACAATCGGACTTATG 428 113 121 Antisense
AGGATCTCCAAATGTTCGTGTCACC 390 57 165 Antisense
GTCACCACGCAAACTCATACTGCAA 665 465 184 Antisense
GGAATGCCGGTAACCGTCGTCGATA 60 533 214 Antisense
GAATCAACCTTCAAACGTCGCTTTC 586 327 295 Antisense
AAAAGCCGTCGCACCGGAATCTGTT 525 131 327 Antisense
ACCGGAATCTGTTCAATCTCAGCTC 183 89 339 Antisense
CAGCTCACTGCTGATGGTCATTTGA 606 203 358 Antisense
GAAGGAGCTCGTCCGATTCAGATTA 102 335 415 Antisense
Sequence Source Affymetrix Proprietary Database