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[177043_at]

Description Y34B4A.E /REP_DB=WormBase Gene ID /WP=CE21521 /CHR=X /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF59640.1
Cluster 6grp_highest_10_10 > 54      6grp_high1_10_10 > 34      4grp_highest_8_8 > 62      4grp_high_8_8 > 26      6grp_highest_nonorm > 80      6grp_total > 30      4grp_total > 30      6grp_cgc > 63      6grp_high1_sub > 02      6grp_cgc_sub > 02      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 141
1 2 3 4 range
A 195
(0.2)
P 		198
(0.4)
P 		142.5
(-3)
P 		239.5
(3)
P 		97
B 254
(3)
P 		201.5
(-0.7)
P 		169.3
(-3)
P 		194.1
(-1.2)
P 		85
C 283.9
(3)
P 		220.2
(-1.4)
P 		196.8
(-3)
P 		218.8
(-1.5)
P 		87
avr 244.3
(3) 		206.6
(-0) 		169.5
(-3) 		217.5
(0.9) 		75

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 189
1 2 3 4 5 6 range
A 164.1
(-3)
P 		349.9
(3)
P 		194.5
(-2)
P 		217.3
(-1.3)
P 		180.6
(-2.5)
P 		199.7
(-1.9)
P 		186
B 266.5
(0.9)
P 		230.7
(-1.3)
P 		209.5
(-2.6)
P 		299.5
(3)
P 		203.3
(-3)
P 		258.4
(0.4)
P 		96
C 220.6
(-0.5)
P 		271.7
(1.7)
P 		222.3
(-0.4)
P 		302.5
(3)
P 		161.2
(-3)
P 		212.7
(-0.8)
P 		141
avr 217.1
(-0.9) 		284.1
(3) 		208.8
(-1.4) 		273.1
(2.4) 		181.7
(-3) 		223.6
(-0.5) 		102
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 59
1 2 3 4 range
A 71
(3)
P 		43
(-1.7)
P 		37.3
(-2.6)
P 		35.1
(-3)
P 		36
B 81
(3)
P 		49.8
(-1.5)
P 		39.2
(-3)
P 		41.4
(-2.7)
P 		42
C 94
(3)
P 		63.3
(-0.2)
P 		68.6
(0.3)
P 		36.9
(-3)
P 		57
avr 82
(3) 		52
(-1.1) 		48.4
(-1.6) 		37.8
(-3) 		44

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 94
1 2 3 4 5 6 range
A 30.3
(-3)
P 		124.4
(3)
P 		42.3
(-2.2)
P 		55.1
(-1.4)
P 		54.5
(-1.5)
P 		48.6
(-1.8)
P 		94
B 92.7
(2.5)
P 		76.8
(0.4)
P 		50.9
(-3)
P 		79.3
(0.7)
P 		82.6
(1.2)
P 		96.4
(3)
P 		46
C 81.8
(2.8)
P 		82.9
(3)
P 		56.6
(-2.1)
P 		60.8
(-1.3)
P 		52.2
(-3)
P 		71.2
(0.7)
P 		31
avr 68.3
(-0.5) 		94.7
(3) 		49.9
(-3) 		65.1
(-1) 		63.1
(-1.2) 		72.1
(-0) 		45
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177043_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19550
Sequence Type Exemplar sequence
Representative Public ID Y34B4A.E NCBI
Target Description Y34B4A.E /REP_DB=WormBase Gene ID /WP=CE21521 /CHR=X /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF59640.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076494
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
GENEFINDER00000022896 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:5275537:5278263:1 None
Y34B4A.3 ENSEMBL cdna:known chromosome:CEL140:X:5275532:5278357:1 gene:Y34B4A.3 None
NM_076494 NCBI Caenorhabditis elegans Heparan SulphoTransferase family member (hst-6) (hst-6) mRNA, complete cds. None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:5275758-5279379(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol hst-6
Ensembl Y34B4A.3
Entrez Gene 189586 Entrez gene
SwissProt Q8T4K5 EMBL-EBI
WormBase CE32011 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.28787.1.S1_S_AT similar to heparan sulfate 6-O-sulfotransferase 2 cfa
CHICKEN:GGA.9499.1.S1_S_AT heparan sulfate 6-O-sulfotransferase 2 gga
CHICKEN:GGAAFFX.20439.1.S1_S_AT heparan sulfate 6-O-sulfotransferase 2 gga
HG-U133_PLUS_2:230030_AT heparan sulfate 6-O-sulfotransferase 2 hs
HG-U133B:230030_AT heparan sulfate 6-O-sulfotransferase 2 hs
HU35KSUBC:RC_AA436616_AT heparan sulfate 6-O-sulfotransferase 2 hs
U133_X3P:HS2.82302.2.S1_3P_S_AT heparan sulfate 6-O-sulfotransferase 2 hs
U133_X3P:HS.82302.0.A1_3P_AT heparan sulfate 6-O-sulfotransferase 2 hs
HG-U133_PLUS_2:1552766_AT heparan sulfate 6-O-sulfotransferase 2 hs
HG-U133_PLUS_2:1552767_A_AT heparan sulfate 6-O-sulfotransferase 2 hs
HG-U95B:52742_AT heparan sulfate 6-O-sulfotransferase 2 hs
MG-U74BV2:165120_AT heparan sulfate 6-O-sulfotransferase 2 mm
MOE430A:1450047_AT heparan sulfate 6-O-sulfotransferase 2 mm
MOUSE430A_2:1450047_AT heparan sulfate 6-O-sulfotransferase 2 mm
MOUSE430_2:1450047_AT heparan sulfate 6-O-sulfotransferase 2 mm
MOE430A:1420938_AT heparan sulfate 6-O-sulfotransferase 2 mm
MOUSE430A_2:1420938_AT heparan sulfate 6-O-sulfotransferase 2 mm
MOUSE430_2:1420938_AT heparan sulfate 6-O-sulfotransferase 2 mm
MOE430A:1420939_AT heparan sulfate 6-O-sulfotransferase 2 mm
MOUSE430A_2:1420939_AT heparan sulfate 6-O-sulfotransferase 2 mm
MOUSE430_2:1420939_AT heparan sulfate 6-O-sulfotransferase 2 mm
MOE430B:1446257_AT heparan sulfate 6-O-sulfotransferase 2 mm
MOUSE430_2:1446257_AT heparan sulfate 6-O-sulfotransferase 2 mm
MG-U74CV2:165765_AT Heparan sulfate 6-O-sulfotransferase 2 (Hs6st2), mRNA mm
MU19KSUBC:TC38378_AT Heparan sulfate 6-O-sulfotransferase 2 (Hs6st2), mRNA mm
Protein Similarities
Method ID Description E-Value
blast AAF59640 Heparan sulphotransferase protein 6 [Caenorhabditis elegans] ref|NP_508895.2| Heparan SulphoTransferase family member (hst-6) [Caenorhabditis elegans] gb|AAL91661.1| heparan 6-O sulfotransferase HST-6 [Caenorhabditis elegans] 0.0
blast CAE68725 Hypothetical protein CBG14651 [Caenorhabditis briggsae] 1.0E-178
blast NP_989821 heparan sulfate 6-O-sulfotransferase 2 [Gallus gallus] dbj|BAD02830.1| heparan sulfate 6-O-sulfotransferase-2 [Gallus gallus] sp|Q76LW2|H6ST2_CHICK Heparan-sulfate 6-O-sulfotransferase 2 (HS6ST-2) (cHS6ST-2) 7.0E-77
Protein Domains
Database ID Description E-Value
Pfam IPR010635 EMBL-EBI Heparan sulphate 6-sulfotransferase 6.9E-120
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_508895.2 1 20-37
Y34B4A.3 1 20-37
GENEFINDER00000022896 1 20-37
Sequence
Target Sequence 
>C. ELEGANS:177043_AT
ctacttctacacaacatttcttcgcaacccaactgatcgatttatctcggaatttcgaca
tgttcaaagaggcgctacatggatatcgtctaagcacgtttgcaatgggaagccagcatc
tgttaatgaccttccgacatgttttgatccccgaattgggtgggaaggtgtttcgctaga
agagtttatttcatgcccgtacaatttggcattcaaccgacaaacgcgaatgttgtccaa
tttatcacttgtcggatgctatgaacatatgagaaagccttcctatgaacaagacaagat
aatgatagaaagtgcgaaagaaaatttaagaaaaatgtcattttttggaatcaaggaaca
aatggacgatagccagttttactttgaaaacacttttgatatgcacaagtcatttccatt
tggaattgataagagaagtttgctatctttttactttcttgttgcattaatacctcttta
tgttacctgctctatatttagttctctcaatgaccaaaacgtctgcgtctctggatctct
ctcacacctcatgttcatcccaaccgtccatgatatcta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTACTTCTACACAACATTTCTTCGC 565 225 384 Antisense
TTTCTTCGCAACCCAACTGATCGAT 140 671 400 Antisense
GAGGCGCTACATGGATATCGTCTAA 174 407 452 Antisense
GCCAGCATCTGTTAATGACCTTCCG 192 279 495 Antisense
TTCCGACATGTTTTGATCCCCGAAT 168 699 515 Antisense
GAGTTTATTTCATGCCCGTACAATT 372 397 565 Antisense
GTTGTCCAATTTATCACTTGTCGGA 623 437 615 Antisense
GTTTGCTATCTTTTTACTTTCTTGT 405 451 821 Antisense
TAATACCTCTTTATGTTACCTGCTC 336 635 851 Antisense
ACCTGCTCTATATTTAGTTCTCTCA 150 89 868 Antisense
TCATCCCAACCGTCCATGATATCTA 26 623 938 Antisense
Sequence Source Affymetrix Proprietary Database