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[177040_at]

Description Y34B4A.D /REP_DB=WormBase Gene ID /WP=CE21520 /CHR=X /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF59639.1
Cluster 6grp_highest_10_10 > 50      6grp_high1_10_10 > 06      4grp_highest_8_8 > 50      4grp_high_8_8 > 40      6grp_highest_nonorm > 80      6grp_total > 05      4grp_total > 47      6grp_highest_sub > De      6grp_high1_sub > De      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 366
1 2 3 4 range
A 481.7
(3)
P 		176.8
(-2.8)
P 		192
(-2.5)
P 		167.8
(-3)
P 		314
B 434.2
(3)
P 		173.2
(-2.2)
P 		143.9
(-2.8)
P 		135.4
(-3)
P 		299
C 397.8
(3)
P 		173.4
(-1.8)
P 		154.5
(-2.2)
P 		115.8
(-3)
P 		282
avr 437.9
(3) 		174.5
(-2.3) 		163.5
(-2.5) 		139.7
(-3) 		298

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 289
1 2 3 4 5 6 range
A 388.5
(3)
P 		241.4
(-0.1)
P 		172.6
(-1.5)
P 		172.2
(-1.6)
P 		147.7
(-2.1)
P 		103.6
(-3)
P 		285
B 322.5
(3)
P 		222.9
(0)
P 		167.7
(-1.6)
P 		168.7
(-1.6)
P 		124.7
(-2.9)
P 		122.4
(-3)
P 		200
C 342.8
(3)
P 		215.3
(-0.2)
P 		234.7
(0.3)
P 		190.4
(-0.8)
P 		126.6
(-2.3)
P 		100
(-3)
P 		243
avr 351.3
(3) 		226.5
(-0.1) 		191.7
(-0.9) 		177.1
(-1.3) 		133
(-2.4) 		108.7
(-3) 		243
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 156
1 2 3 4 range
A 175.4
(3)
P 		38.4
(-2.5)
P 		50.2
(-2)
P 		24.6
(-3)
P 		151
B 138.5
(3)
P 		42.8
(-2.2)
P 		33.4
(-2.7)
P 		28.8
(-3)
P 		110
C 131.7
(3)
P 		49.9
(-1.4)
P 		53.8
(-1.2)
P 		19.5
(-3)
P 		112
avr 148.5
(3) 		43.7
(-2.1) 		45.8
(-2) 		24.3
(-3) 		124

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 102
1 2 3 4 5 6 range
A 71.6
(1.6)
P 		85.9
(3)
P 		37.6
(-1.8)
P 		43.7
(-1.2)
P 		44.6
(-1.1)
P 		25.2
(-3)
P 		61
B 112.2
(3)
P 		74.2
(-0.2)
P 		40.7
(-3)
P 		44.7
(-2.7)
P 		50.7
(-2.2)
P 		45.7
(-2.6)
P 		72
C 127.1
(3)
P 		65.7
(-0.9)
P 		59.7
(-1.3)
P 		38.3
(-2.7)
P 		41
(-2.5)
P 		33.5
(-3)
P 		94
avr 103.6
(3) 		75.3
(0.5) 		46
(-2) 		42.2
(-2.4) 		45.4
(-2.1) 		34.8
(-3) 		69
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177040_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19551
Sequence Type Exemplar sequence
Representative Public ID Y34B4A.D NCBI
Target Description Y34B4A.D /REP_DB=WormBase Gene ID /WP=CE21520 /CHR=X /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF59639.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001029788(11), NM_001029787(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001029788 NCBI Caenorhabditis elegans Y34B4A.4b (Y34B4A.4) mRNA, complete cds. 11/11 None
NM_001029787 NCBI Caenorhabditis elegans Y34B4A.4a (Y34B4A.4) mRNA, complete cds. 11/11 None
GENEFINDER00000022888 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:5264295:5271607:1 11/11 None
SNAP00000022882 ENSEMBL cdna:SNAP chromosome:CEL140:X:5268426:5271607:1 11/11 None
Y34B4A.4b ENSEMBL cdna:known chromosome:CEL140:X:5264295:5271753:1 gene:Y34B4A.4 11/11 A 
Y34B4A.4a ENSEMBL cdna:known chromosome:CEL140:X:5264295:5271607:1 gene:Y34B4A.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:5264295-5271608(+) 100.0 100.0
Public Domain and Genome References
Ensembl Y34B4A.4
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAF59639 Hypothetical protein Y34B4A.4a [Caenorhabditis elegans] ref|NP_001024958.1| Y34B4A.4a [Caenorhabditis elegans] 0.0
blast AAO91688 Hypothetical protein Y34B4A.4b [Caenorhabditis elegans] ref|NP_001024959.1| Y34B4A.4b [Caenorhabditis elegans] 0.0
blast CAE68723 Hypothetical protein CBG14649 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001895 EMBL-EBI Guanine-nucleotide dissociation stimulator CDC25 1.4E-39
Pfam IPR000651 EMBL-EBI Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal motif 7.6E-12
Pfam IPR001895 EMBL-EBI Guanine-nucleotide dissociation stimulator CDC25 1.4E-39
Pfam IPR000651 EMBL-EBI Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal motif 1.1E-10
Pfam IPR001895 EMBL-EBI Guanine-nucleotide dissociation stimulator CDC25 1.4E-39
Pfam IPR000651 EMBL-EBI Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal motif 7.6E-12
Sequence
Target Sequence 
>C. ELEGANS:177040_AT
agttcaataatctgtccttctgggtacgaacactgatcatcactcaaggaactcaaaaag
aaagggagcgacacatgatgaaatttgttaaaattatgaagcatctccgcgaaatgtgca
atttcaactcttatttggcaattctcagcgcaattatgtccacgccattggctaggttgg
aatggagcaaaaatatcaaagatgctctaaaggagcacactgcagttatggacacagctc
agtcgtacaaaaattacagagcactgttacaaacagcgaagccgccatgtgttccttata
tcggcattatcctccaagacttgacatttgttcacgccggcaacccagacactattccat
ccgaccgatgccacggcgccaagaacatgatcaacttcctgaaacggtggaaccagtttg
ccattcttgacagcatccgcaaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGTTCAATAATCTGTCCTTCTGGGT 10 61 2057 Antisense
TGGGTACGAACACTGATCATCACTC 656 553 2077 Antisense
TTCTCAGCGCAATTATGTCCACGCC 530 691 2198 Antisense
TGTCCACGCCATTGGCTAGGTTGGA 39 555 2213 Antisense
GGAGCACACTGCAGTTATGGACACA 203 521 2268 Antisense
AGCCGCCATGTGTTCCTTATATCGG 571 81 2336 Antisense
TTATATCGGCATTATCCTCCAAGAC 199 679 2352 Antisense
AAGACTTGACATTTGTTCACGCCGG 628 151 2372 Antisense
TGCCACGGCGCCAAGAACATGATCA 391 587 2425 Antisense
ACGGTGGAACCAGTTTGCCATTCTT 187 93 2460 Antisense
TGCCATTCTTGACAGCATCCGCAAA 617 585 2475 Antisense
Sequence Source Affymetrix Proprietary Database