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[177035_at]

Description Y48G9A.G /REP_DB=WormBase Gene ID /WP=CE22774 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF60713.1
Cluster 4grp_high_8_8 > 34      6grp_total > 28      4grp_total > 37      6grp_cgc > 12      6grp_cgc_sub > De      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 154
1 2 3 4 range
A 250.2
(3)
P 		176.6
(0.1)
P 		128.2
(-1.8)
A 		96.7
(-3)
A 		154
B 222.5
(3)
P 		188.8
(1.3)
P 		104.7
(-3)
P 		132.9
(-1.6)
P 		118
C 242
(3)
P 		222.1
(1.6)
P 		153.7
(-3)
P 		241.3
(3)
A 		88
avr 238.2
(3) 		195.8
(0.7) 		128.9
(-3) 		157
(-1.5) 		109

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 117
1 2 3 4 5 6 range
A 161.7
(1.7)
P 		183.9
(3)
P 		149.6
(1)
P 		78.6
(-3)
A 		123.2
(-0.5)
P 		116.4
(-0.8)
P 		105
B 195.7
(3)
P 		138.9
(-3)
P 		162.4
(-0.5)
P 		153.9
(-1.4)
A 		152
(-1.6)
P 		167.7
(0)
P 		57
C 183.8
(3)
P 		118.2
(-3)
P 		126.7
(-2.2)
P 		129.9
(-1.9)
A 		179.6
(2.6)
M 		119.7
(-2.9)
P 		66
avr 180.4
(3) 		147
(-0.4) 		146.2
(-0.4) 		120.8
(-3) 		151.6
(0.1) 		134.6
(-1.6) 		60
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 77
1 2 3 4 range
A 91.1
(3)
P 		38.3
(-1.1)
P 		33.5
(-1.5)
A 		14.2
(-3)
A 		77
B 70.9
(3)
P 		46.6
(-0.1)
P 		24.3
(-3)
P 		28.3
(-2.5)
P 		47
C 80.1
(3)
P 		63.8
(0.5)
P 		53.6
(-1)
P 		40.7
(-3)
A 		39
avr 80.7
(3) 		49.6
(-0.5) 		37.1
(-1.9) 		27.7
(-3) 		53

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 48
1 2 3 4 5 6 range
A 29.8
(-1.7)
P 		65.4
(3)
P 		32.6
(-1.3)
P 		19.9
(-3)
A 		37.2
(-0.7)
P 		28.3
(-1.9)
P 		46
B 68.1
(3)
P 		46.2
(-1.6)
P 		39.4
(-3)
P 		40.8
(-2.7)
A 		61.8
(1.7)
P 		62.5
(1.8)
P 		29
C 68.1
(3)
P 		36.1
(-1.6)
P 		32.2
(-2.1)
P 		26.1
(-3)
A 		58.1
(1.6)
M 		40.1
(-1)
P 		42
avr 55.3
(3) 		49.2
(1.6) 		34.7
(-1.7) 		28.9
(-3) 		52.4
(2.3) 		43.6
(0.3) 		26
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177035_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19035
Sequence Type Exemplar sequence
Representative Public ID Y48G9A.G NCBI
Target Description Y48G9A.G /REP_DB=WormBase Gene ID /WP=CE22774 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF60713.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065099(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065099 NCBI Caenorhabditis elegans PIP Kinase family member (ppk-2) (ppk-2) mRNA, complete cds. 9/11 A 
GENEFINDER00000015842 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:2102305:2105055:1 9/11 None
Y48G9A.8.2 ENSEMBL cdna:known chromosome:CEL140:III:2102282:2107403:1 gene:Y48G9A.8 9/11 None
Y48G9A.8.1 ENSEMBL cdna:known chromosome:CEL140:III:2102283:2107458:1 gene:Y48G9A.8 9/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:2102304-2103924(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol ppk-2
Ensembl Y48G9A.8
Entrez Gene 175344 Entrez gene
SwissProt Q9BL73 EMBL-EBI
WormBase CE27279 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1588133_AT similar to Phosphatidylinositol-4-phosphate 5-kinase type II alpha (PIP5KII-alpha) (1-phosphatidylinositol-4-phosphate 5-kinase) (PtdIns(4)P-5-kinase B isoform) (Diphosphoinositide kinase) cfa
CANINE_2:CFA.6094.1.S1_AT similar to Phosphatidylinositol-4-phosphate 5-kinase type II alpha (PIP5KII-alpha) (1-phosphatidylinositol-4-phosphate 5-kinase) (PtdIns(4)P-5-kinase B isoform) (Diphosphoinositide kinase) cfa
CANINE_2:CFAAFFX.7216.1.S1_AT similar to Phosphatidylinositol-4-phosphate 5-kinase type II alpha (PIP5KII-alpha) (1-phosphatidylinositol-4-phosphate 5-kinase) (PtdIns(4)P-5-kinase B isoform) (Diphosphoinositide kinase) cfa
CANINE_2:CFAAFFX.7216.1.S1_S_AT similar to Phosphatidylinositol-4-phosphate 5-kinase type II alpha (PIP5KII-alpha) (1-phosphatidylinositol-4-phosphate 5-kinase) (PtdIns(4)P-5-kinase B isoform) (Diphosphoinositide kinase) cfa
CHICKEN:GGAAFFX.4938.1.S1_AT phosphatidylinositol-4-phosphate 5-kinase, type II, alpha gga
HG-U133A:205570_AT phosphatidylinositol-4-phosphate 5-kinase, type II, alpha hs
HG-U133_PLUS_2:205570_AT phosphatidylinositol-4-phosphate 5-kinase, type II, alpha hs
HG-FOCUS:205570_AT phosphatidylinositol-4-phosphate 5-kinase, type II, alpha hs
HG-U133A_2:205570_AT phosphatidylinositol-4-phosphate 5-kinase, type II, alpha hs
HU35KSUBC:RC_T40568_S_AT phosphatidylinositol-4-phosphate 5-kinase, type II, alpha hs
HU35KSUBD:RC_N24948_S_AT phosphatidylinositol-4-phosphate 5-kinase, type II, alpha hs
HUGENEFL:S78798_S_AT phosphatidylinositol-4-phosphate 5-kinase, type II, alpha hs
U133_X3P:G6857819_3P_AT phosphatidylinositol-4-phosphate 5-kinase, type II, alpha hs
HG-U95AV2:37412_AT phosphatidylinositol-4-phosphate 5-kinase, type II, alpha hs
HG-U95D:87191_R_AT Phosphatidylinositol-4-phosphate 5-kinase, type II, alpha hs
HG-U133_PLUS_2:241155_AT Phosphatidylinositol-4-phosphate 5-kinase, type II, alpha hs
HG-U133B:241155_AT Phosphatidylinositol-4-phosphate 5-kinase, type II, alpha hs
HG-U95B:55298_AT Phosphatidylinositol-4-phosphate 5-kinase, type II, alpha hs
HG-U95C:56421_AT Phosphatidylinositol-4-phosphate 5-kinase, type II, alpha hs
HG-U95D:72321_AT Phosphatidylinositol-4-phosphate 5-kinase, type II, alpha hs
HG-U95D:80529_R_AT Phosphatidylinositol-4-phosphate 5-kinase, type II, alpha hs
U133_X3P:HS.58934.0.A1_3P_AT Phosphatidylinositol-4-phosphate 5-kinase, type II, alpha hs
HU35KSUBC:RC_W90527_AT Phosphatidylinositol-4-phosphate 5-kinase, type II, alpha hs
MU11KSUBA:AA260699_S_AT phosphatidylinositol-4-phosphate 5-kinase, type II, alpha mm
MU11KSUBB:MSA.19352.0_S_AT phosphatidylinositol-4-phosphate 5-kinase, type II, alpha mm
MG-U74AV2:101865_AT phosphatidylinositol-4-phosphate 5-kinase, type II, alpha mm
MG-U74AV2:95358_AT phosphatidylinositol-4-phosphate 5-kinase, type II, alpha mm
MG-U74BV2:114956_AT phosphatidylinositol-4-phosphate 5-kinase, type II, alpha mm
MG-U74AV2:162503_F_AT phosphatidylinositol-4-phosphate 5-kinase, type II, alpha mm
MG-U74CV2:171196_I_AT phosphatidylinositol-4-phosphate 5-kinase, type II, alpha mm
MOE430A:1419280_AT phosphatidylinositol-4-phosphate 5-kinase, type II, alpha mm
MOUSE430A_2:1419280_AT phosphatidylinositol-4-phosphate 5-kinase, type II, alpha mm
MOUSE430_2:1419280_AT phosphatidylinositol-4-phosphate 5-kinase, type II, alpha mm
MOE430A:1449404_AT phosphatidylinositol-4-phosphate 5-kinase, type II, alpha mm
MOUSE430A_2:1449404_AT phosphatidylinositol-4-phosphate 5-kinase, type II, alpha mm
MOUSE430_2:1449404_AT phosphatidylinositol-4-phosphate 5-kinase, type II, alpha mm
MOE430A:1419279_AT phosphatidylinositol-4-phosphate 5-kinase, type II, alpha mm
MOUSE430A_2:1419279_AT phosphatidylinositol-4-phosphate 5-kinase, type II, alpha mm
MOUSE430_2:1419279_AT phosphatidylinositol-4-phosphate 5-kinase, type II, alpha mm
MOUSE430_2:1444159_AT Phosphatidylinositol-4-phosphate 5-kinase, type II, alpha, mRNA (cDNA clone MGC:18357 IMAGE:3672732) mm
MOE430B:1444159_AT Phosphatidylinositol-4-phosphate 5-kinase, type II, alpha, mRNA (cDNA clone MGC:18357 IMAGE:3672732) mm
MG-U74AV2:101116_AT Phosphatidylinositol-4-phosphate 5-kinase, type II, alpha, mRNA (cDNA clone MGC:18357 IMAGE:3672732) mm
MOE430A:1420109_AT Phosphatidylinositol-4-phosphate 5-kinase, type II, alpha, mRNA (cDNA clone MGC:18357 IMAGE:3672732) mm
MOUSE430A_2:1420109_AT Phosphatidylinositol-4-phosphate 5-kinase, type II, alpha, mRNA (cDNA clone MGC:18357 IMAGE:3672732) mm
MOUSE430_2:1420109_AT Phosphatidylinositol-4-phosphate 5-kinase, type II, alpha, mRNA (cDNA clone MGC:18357 IMAGE:3672732) mm
MOUSE430_2:1442310_AT Phosphatidylinositol-4-phosphate 5-kinase, type II, alpha, mRNA (cDNA clone MGC:18357 IMAGE:3672732) mm
MOE430B:1442310_AT Phosphatidylinositol-4-phosphate 5-kinase, type II, alpha, mRNA (cDNA clone MGC:18357 IMAGE:3672732) mm
MOE430A:1449736_AT Phosphatidylinositol-4-phosphate 5-kinase, type II, alpha, mRNA (cDNA clone MGC:18357 IMAGE:3672732) mm
MOUSE430A_2:1449736_AT Phosphatidylinositol-4-phosphate 5-kinase, type II, alpha, mRNA (cDNA clone MGC:18357 IMAGE:3672732) mm
MOUSE430_2:1449736_AT Phosphatidylinositol-4-phosphate 5-kinase, type II, alpha, mRNA (cDNA clone MGC:18357 IMAGE:3672732) mm
MU19KSUBA:TC17510_AT Phosphatidylinositol-4-phosphate 5-kinase, type II, alpha, mRNA (cDNA clone MGC:18357 IMAGE:3672732) mm
MU19KSUBB:TC32019_AT Phosphatidylinositol-4-phosphate 5-kinase, type II, alpha, mRNA (cDNA clone MGC:18357 IMAGE:3672732) mm
MU19KSUBB:TC34798_F_AT Phosphatidylinositol-4-phosphate 5-kinase, type II, alpha, mRNA (cDNA clone MGC:18357 IMAGE:3672732) mm
MU19KSUBC:TC40401_AT Phosphatidylinositol-4-phosphate 5-kinase, type II, alpha, mRNA (cDNA clone MGC:18357 IMAGE:3672732) mm
MU11KSUBA:AA511245_RC_AT Phosphatidylinositol-4-phosphate 5-kinase, type II, alpha, mRNA (cDNA clone MGC:18357 IMAGE:3672732) mm
RG-U34B:RC_AI058767_AT phosphatidylinositol-4-phosphate 5-kinase, type II, alpha rn
RAE230A:1387412_AT phosphatidylinositol-4-phosphate 5-kinase, type II, alpha rn
RAT230_2:1387412_AT phosphatidylinositol-4-phosphate 5-kinase, type II, alpha rn
RAE230A:1389109_AT Phosphatidylinositol-4-phosphate 5-kinase, type II, alpha rn
RAT230_2:1389109_AT Phosphatidylinositol-4-phosphate 5-kinase, type II, alpha rn
RG-U34B:RC_AA943238_AT Phosphatidylinositol-4-phosphate 5-kinase, type II, alpha rn
RG-U34B:RC_AI059779_AT Phosphatidylinositol-4-phosphate 5-kinase, type II, alpha rn
RG-U34C:RC_AI172519_AT Phosphatidylinositol-4-phosphate 5-kinase, type II, alpha rn
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
16308 1-phosphatidylinositol-4-phosphate 5-kinase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK29922 Pip kinase protein 2 [Caenorhabditis elegans] ref|NP_497500.1| PIP Kinase family member (ppk-2) [Caenorhabditis elegans] 0.0
blast CAE69090 Hypothetical protein CBG15109 [Caenorhabditis briggsae] 0.0
blast AAK29922 Pip kinase protein 2 [Caenorhabditis elegans] ref|NP_497500.1| PIP Kinase family member (ppk-2) [Caenorhabditis elegans] 1.0E-149
blast CAE69090 Hypothetical protein CBG15109 [Caenorhabditis briggsae] 1.0E-146
blast AAL68342 RH07911p [Drosophila melanogaster] 1.0E-112
Protein Domains
Database ID Description E-Value
Pfam IPR002498 EMBL-EBI Phosphatidylinositol-4-phosphate 5-kinase 1.7E-53
Pfam IPR002498 EMBL-EBI Phosphatidylinositol-4-phosphate 5-kinase 2.6E-80
Sequence
Target Sequence 
>C. ELEGANS:177035_AT
ctgtccaaatgtgttccgtaaccttcgtgagcaatttggtgtcgacaatttcgaatatct
acgctcgctgacgtcatacgagccggagcccgacttgttggatggctcggcaaaggattc
cacgccgagattctttatatcgtatgataagaagtttgtgatcaaggtttgtgtgtggag
aaatcgagaaaatcgtaggattttgaccgaaaaaatgcgaaaattcggcttcaaagtgca
taaaaacgtaaattttaggcataaaaacgtaaattttaagcttaaaaacgtgaattttaa
gcctaaaaccgtgaattttaagcctaaaaccgtgaattttaagcctaaaaacgtgaattt
taggcctaaaaacgtaaaatttagccctaaaaactgtgaaaccgtgaattttaagcctaa
aaacttaaattttaagcctaaaaacctaaattttaggcctgaaaacgtaaattttaagcc
taaaaactcaatggactccgaagcggtcgccgagctccactcagtactccggaattatc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTGTCCAAATGTGTTCCGTAACCTT 595 215 273 Antisense
GTTCCGTAACCTTCGTGAGCAATTT 122 441 285 Antisense
GAGCAATTTGGTGTCGACAATTTCG 299 389 301 Antisense
TTTCGAATATCTACGCTCGCTGACG 219 669 321 Antisense
CTCGCTGACGTCATACGAGCCGGAG 136 233 336 Antisense
AGCCCGACTTGTTGGATGGCTCGGC 423 81 359 Antisense
GATGGCTCGGCAAAGGATTCCACGC 521 405 373 Antisense
GGATTCCACGCCGAGATTCTTTATA 108 509 387 Antisense
GCGAAAATTCGGCTTCAAAGTGCAT 363 287 489 Antisense
CTCAATGGACTCCGAAGCGGTCGCC 361 227 759 Antisense
CTCCACTCAGTACTCCGGAATTATC 116 235 787 Antisense
Sequence Source Affymetrix Proprietary Database