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[177033_at]

Description Y55D5A.I /REP_DB=WormBase Gene ID /WP=CE22520 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAG23458.1
Cluster 6grp_highest_10_10 > 01      6grp_high1_10_10 > 0      4grp_highest_8_8 > 40      4grp_high_8_8 > 43      6grp_highest_nonorm > 80      6grp_total > 13      4grp_total > 44      6grp_total_sub > Decrease      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 141
1 2 3 4 range
A 309.3
(2.7)
P 		314
(3)
P 		285.3
(1.1)
P 		223.8
(-3)
P 		90
B 273.7
(3)
P 		265.8
(2.5)
P 		199.4
(-1.4)
P 		172.8
(-3)
P 		101
C 299.4
(2.7)
P 		305.4
(3)
P 		210.6
(-1.4)
P 		177.1
(-3)
P 		128
avr 294.1
(2.9) 		295.1
(3) 		231.8
(-0.7) 		191.2
(-3) 		104

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 225
1 2 3 4 5 6 range
A 264
(2.9)
P 		227
(1.2)
P 		218.4
(0.8)
P 		266.9
(3)
P 		209.8
(0.4)
P 		133.1
(-3)
P 		134
B 343
(3)
P 		215.7
(-0.4)
P 		216.6
(-0.4)
P 		245.9
(0.4)
P 		197.7
(-0.9)
P 		118.5
(-3)
P 		225
C 270.7
(1.9)
P 		240.3
(0.9)
P 		233.3
(0.6)
P 		301.3
(3)
P 		201.5
(-0.5)
P 		129.8
(-3)
P 		172
avr 292.6
(3) 		227.7
(0.6) 		222.8
(0.5) 		271.4
(2.2) 		203
(-0.2) 		127.1
(-3) 		166
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 83
1 2 3 4 range
A 112.6
(3)
P 		68.2
(-0.3)
P 		74.6
(0.1)
P 		32.8
(-3)
P 		80
B 87.3
(3)
P 		65.7
(0.4)
P 		46.2
(-1.9)
P 		36.8
(-3)
P 		51
C 99.1
(3)
P 		87.8
(2)
P 		73.4
(0.8)
P 		29.9
(-3)
P 		69
avr 99.7
(3) 		73.9
(0.7) 		64.7
(-0.2) 		33.2
(-3) 		67

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 87
1 2 3 4 5 6 range
A 48.7
(-1)
P 		80.7
(3)
P 		47.6
(-1.1)
P 		67.7
(1.4)
P 		63.3
(0.8)
P 		32.4
(-3)
P 		48
B 119.4
(3)
P 		71.8
(-0.8)
P 		52.6
(-2.3)
P 		65.1
(-1.3)
P 		80.3
(-0.1)
P 		44.2
(-3)
P 		75
C 100.3
(3)
P 		73.3
(0.1)
P 		59.4
(-1.3)
P 		60.6
(-1.2)
P 		65.2
(-0.7)
P 		43.5
(-3)
P 		57
avr 89.5
(3) 		75.3
(1.3) 		53.2
(-1.4) 		64.5
(-0) 		69.6
(0.6) 		40
(-3) 		50
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177033_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18962
Sequence Type Exemplar sequence
Representative Public ID Y55D5A.I NCBI
Target Description Y55D5A.I /REP_DB=WormBase Gene ID /WP=CE22520 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAG23458.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001027748(11), NM_001027749(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001027748 NCBI Caenorhabditis elegans Y55D5A.1a (Y55D5A.1) mRNA, complete cds. 11/11 None
NM_001027749 NCBI Caenorhabditis elegans Y55D5A.1b (Y55D5A.1) mRNA, complete cds. 11/11 None
GENEFINDER00000010560 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:2982461:2987975:1 11/11 None
Y55D5A.1a ENSEMBL cdna:known chromosome:CEL140:III:2982413:2988068:1 gene:Y55D5A.1 11/11 A 
Y55D5A.1b ENSEMBL cdna:known chromosome:CEL140:III:2985996:2987975:1 gene:Y55D5A.1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000010551 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:2986077-2987975(+) 100.0 100.0
Public Domain and Genome References
Ensembl Y55D5A.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK39621 Hypothetical protein Y55D5A.1a [Caenorhabditis elegans] ref|NP_001022919.1| Y55D5A.1a [Caenorhabditis elegans] 1.0E-135
blast CAE57501 Hypothetical protein CBG00474 [Caenorhabditis briggsae] 9.0E-95
blast AAP40511 Hypothetical protein Y55D5A.1b [Caenorhabditis elegans] ref|NP_001022920.1| Y55D5A.1b [Caenorhabditis elegans] 3.0E-91
blast AAK39621 Hypothetical protein Y55D5A.1a [Caenorhabditis elegans] ref|NP_001022919.1| Y55D5A.1a [Caenorhabditis elegans] 3.0E-91
Sequence
Target Sequence 
>C. ELEGANS:177033_AT
gttcagcaaggcctagagctcttccgcccgccgacctgcccgaccgcagaacttctccgt
tcaacagctgaaaaacttgaggaaatcggagaatcttcactatctgacaagctaaacaac
aatccagataccctacaaaacgccacatggcaaaccatgaataccgtactccgagtgttc
tcactgaccgatcggcaagtgatgggatacctccgtcacctcaaatcccagacgccctcc
gccatgccaatttttccacggcacctagacacgtgtaatacgtatattgcacttggagat
cacggagcatatttctttgggcaggattttgtgtgcagggctccaatgccgaatggcaca
cagcagaggagaaatgtagtggatcagcctgccaacgctgaaaccggctccgacgtcttc
tggtacgaggcat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTTCAGCAAGGCCTAGAGCTCTTCC 333 445 34 Antisense
AGAACTTCTCCGTTCAACAGCTGAA 70 73 81 Antisense
ATACCCTACAAAACGCCACATGGCA 340 21 161 Antisense
AATACCGTACTCCGAGTGTTCTCAC 275 173 193 Antisense
TGTTCTCACTGACCGATCGGCAAGT 181 563 209 Antisense
CAAGTGATGGGATACCTCCGTCACC 326 183 229 Antisense
CGCCATGCCAATTTTTCCACGGCAC 705 257 273 Antisense
CACGGAGCATATTTCTTTGGGCAGG 458 199 334 Antisense
TGTGTGCAGGGCTCCAATGCCGAAT 206 555 363 Antisense
GGATCAGCCTGCCAACGCTGAAACC 365 511 414 Antisense
TCCGACGTCTTCTGGTACGAGGCAT 21 591 442 Antisense
Sequence Source Affymetrix Proprietary Database