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[177031_at]

Description Y48G9A.O /REP_DB=WormBase Gene ID /WP=CE25332 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF60721.1
Cluster 6grp_highest_10_10 > 72      6grp_high1_10_10 > 28      4grp_highest_8_8 > 49      4grp_high_8_8 > 42      6grp_highest_nonorm > 50      6grp_total > 27      4grp_total > 45      6grp_highest_sub > Cu      6grp_high1_sub > De      6grp_total_sub > Decrease      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 603
1 2 3 4 range
A 1040.5
(3)
P 		952.1
(1.5)
P 		841.3
(-0.4)
P 		692.5
(-3)
P 		348
B 896.9
(3)
P 		760.8
(0.2)
P 		620.4
(-2.6)
P 		600.8
(-3)
P 		296
C 1077.9
(3)
P 		701.1
(-0.7)
P 		558.2
(-2.2)
P 		474.6
(-3)
P 		603
avr 1005.1
(3) 		804.7
(0.1) 		673.3
(-1.8) 		589.3
(-3) 		416

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 511
1 2 3 4 5 6 range
A 675.5
(3)
P 		489.4
(-0.1)
P 		312.9
(-3)
P 		332.7
(-2.7)
P 		415.2
(-1.3)
P 		532.5
(0.6)
P 		363
B 679.7
(3)
P 		561.8
(0.6)
P 		384.4
(-3)
P 		400.5
(-2.7)
P 		403.5
(-2.6)
P 		559
(0.5)
P 		295
C 824.2
(3)
P 		553.3
(-0.4)
P 		472.5
(-1.4)
P 		345.6
(-3)
P 		445.1
(-1.8)
P 		407.5
(-2.2)
P 		479
avr 726.5
(3) 		534.8
(-0.1) 		389.9
(-2.5) 		359.6
(-3) 		421.3
(-2) 		499.7
(-0.7) 		367
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 299
1 2 3 4 range
A 378.8
(3)
P 		206.7
(-0.7)
P 		219.9
(-0.4)
P 		101.4
(-3)
P 		277
B 286
(3)
P 		187.9
(-0.7)
P 		143.8
(-2.4)
P 		128
(-3)
P 		158
C 356.8
(3)
P 		201.6
(-0.4)
P 		194.6
(-0.5)
P 		80
(-3)
P 		277
avr 340.5
(3) 		198.7
(-0.6) 		186.1
(-0.9) 		103.1
(-3) 		237

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 237
1 2 3 4 5 6 range
A 124.5
(0.2)
P 		174.1
(3)
P 		68.1
(-3)
P 		84.4
(-2.1)
P 		125.3
(0.2)
P 		129.6
(0.5)
P 		106
B 236.5
(3)
P 		187
(0.9)
P 		93.3
(-3)
P 		106.1
(-2.5)
P 		163.9
(-0)
P 		208.5
(1.8)
P 		143
C 305.5
(3)
P 		168.8
(-0.5)
P 		120.2
(-1.7)
P 		69.5
(-3)
P 		144
(-1.1)
P 		136.4
(-1.3)
P 		236
avr 222.2
(3) 		176.6
(1) 		93.9
(-2.7) 		86.7
(-3) 		144.4
(-0.4) 		158.2
(0.2) 		136
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177031_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18986
Sequence Type Exemplar sequence
Representative Public ID Y48G9A.O NCBI
Target Description Y48G9A.O /REP_DB=WormBase Gene ID /WP=CE25332 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF60721.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065108(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065108 NCBI Caenorhabditis elegans Y48G9A.1 (Y48G9A.1) mRNA, complete cds. 11/11 None
GENEFINDER00000015836 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:2157639:2197682:1 11/11 None
Y48G9A.1 ENSEMBL cdna:known chromosome:CEL140:III:2174661:2197886:1 gene:Y48G9A.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:2173103-2197682(+) 98.27 100.0
Public Domain and Genome References
Gene Title
Ensembl Y48G9A.1
Entrez Gene 175347 Entrez gene
SwissProt Q9BL71 EMBL-EBI
WormBase CE30024 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:262859_AT translational activator family protein at
ATGENOME1:19030_AT translational activator family protein at
CANINE:1591728_AT similar to GCN1 general control of amino-acid synthesis 1-like 1 cfa
CANINE:1584523_AT similar to GCN1 general control of amino-acid synthesis 1-like 1 cfa
CANINE_2:CFA.4576.1.A1_AT similar to GCN1 general control of amino-acid synthesis 1-like 1 cfa
CANINE_2:CFA.8270.1.A1_AT similar to GCN1 general control of amino-acid synthesis 1-like 1 cfa
CANINE_2:CFA.8270.1.A1_S_AT similar to GCN1 general control of amino-acid synthesis 1-like 1 cfa
CANINE_2:CFAAFFX.15986.1.S1_S_AT similar to GCN1 general control of amino-acid synthesis 1-like 1 cfa
CHICKEN:GGA.17169.1.S1_S_AT GCN1 general control of amino-acid synthesis 1-like 1 (yeast) gga
CHICKEN:GGAAFFX.22198.1.S1_S_AT GCN1 general control of amino-acid synthesis 1-like 1 (yeast) gga
HG-U133_PLUS_2:216232_S_AT GCN1 general control of amino-acid synthesis 1-like 1 (yeast) hs
HG-U133A_2:216232_S_AT GCN1 general control of amino-acid synthesis 1-like 1 (yeast) hs
HG-U133A:216232_S_AT GCN1 general control of amino-acid synthesis 1-like 1 (yeast) hs
HU35KSUBD:RC_T79598_S_AT GCN1 general control of amino-acid synthesis 1-like 1 (yeast) hs
HUGENEFL:D86973_AT GCN1 general control of amino-acid synthesis 1-like 1 (yeast) hs
U133_X3P:HS.75354.2.S1_3P_A_AT GCN1 general control of amino-acid synthesis 1-like 1 (yeast) hs
U133_X3P:HS.75354.0.S1_3P_AT GCN1 general control of amino-acid synthesis 1-like 1 (yeast) hs
HG-U133A_2:212139_AT GCN1 general control of amino-acid synthesis 1-like 1 (yeast) hs
HG-U133_PLUS_2:212139_AT GCN1 general control of amino-acid synthesis 1-like 1 (yeast) hs
HG-U133A:212139_AT GCN1 general control of amino-acid synthesis 1-like 1 (yeast) hs
HG-U95AV2:36603_AT GCN1 general control of amino-acid synthesis 1-like 1 (yeast) hs
MOUSE430_2:1439472_AT GCN1 general control of amino-acid synthesis 1-like 1 (yeast) mm
MOE430B:1439472_AT GCN1 general control of amino-acid synthesis 1-like 1 (yeast) mm
MG-U74BV2:106525_AT GCN1 general control of amino-acid synthesis 1-like 1 (yeast) mm
MG-U74BV2:113627_AT GCN1 general control of amino-acid synthesis 1-like 1 (yeast) mm
MOE430B:1433713_AT GCN1 general control of amino-acid synthesis 1-like 1 (yeast) mm
MOUSE430_2:1433713_AT GCN1 general control of amino-acid synthesis 1-like 1 (yeast) mm
MOE430B:1455879_AT GCN1 general control of amino-acid synthesis 1-like 1 (yeast) mm
MOUSE430_2:1455879_AT GCN1 general control of amino-acid synthesis 1-like 1 (yeast) mm
MOE430B:1446543_AT GCN1 general control of amino-acid synthesis 1-like 1 (yeast) mm
MOUSE430_2:1446543_AT GCN1 general control of amino-acid synthesis 1-like 1 (yeast) mm
MOE430B:1440391_AT GCN1 general control of amino-acid synthesis 1-like 1 (yeast), mRNA (cDNA clone IMAGE:6853233) mm
MOUSE430_2:1440391_AT GCN1 general control of amino-acid synthesis 1-like 1 (yeast), mRNA (cDNA clone IMAGE:6853233) mm
MG-U74CV2:136432_AT GCN1 general control of amino-acid synthesis 1-like 1 (yeast), mRNA (cDNA clone IMAGE:6853233) mm
MOUSE430_2:1446991_AT GCN1 general control of amino-acid synthesis 1-like 1 (yeast), mRNA (cDNA clone IMAGE:6853233) mm
MOE430B:1446991_AT GCN1 general control of amino-acid synthesis 1-like 1 (yeast), mRNA (cDNA clone IMAGE:6853233) mm
MU11KSUBB:MSA.33225.0_S_AT GCN1 general control of amino-acid synthesis 1-like 1 (yeast), mRNA (cDNA clone IMAGE:6853233) mm
MU19KSUBA:TC24035_AT GCN1 general control of amino-acid synthesis 1-like 1 (yeast), mRNA (cDNA clone IMAGE:6853233) mm
MU19KSUBB:TC31270_AT GCN1 general control of amino-acid synthesis 1-like 1 (yeast), mRNA (cDNA clone IMAGE:6853233) mm
RG-U34A:RC_AA800026_AT GCN1 general control of amino-acid synthesis 1-like 1 (yeast) (predicted) rn
RAE230A:1375288_AT GCN1 general control of amino-acid synthesis 1-like 1 (yeast) (predicted) rn
RAT230_2:1375288_AT GCN1 general control of amino-acid synthesis 1-like 1 (yeast) (predicted) rn
RAE230A:1375135_AT GCN1 general control of amino-acid synthesis 1-like 1 (yeast) (predicted) rn
RAT230_2:1375135_AT GCN1 general control of amino-acid synthesis 1-like 1 (yeast) (predicted) rn
RAE230B:1381529_AT GCN1 general control of amino-acid synthesis 1-like 1 (yeast) (predicted) rn
RAT230_2:1381529_AT GCN1 general control of amino-acid synthesis 1-like 1 (yeast) (predicted) rn
RG-U34B:RC_AA850649_AT GCN1 general control of amino-acid synthesis 1-like 1 (yeast) (predicted) rn
YEAST_2:1769954_AT Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn20p; proposed to stimulate Gcn2p activation by an uncharged tRNA Sc
Protein Similarities
Method ID Description E-Value
blast AAK29920 Hypothetical protein Y48G9A.1 [Caenorhabditis elegans] ref|NP_497509.2| Y48G9A.1 [Caenorhabditis elegans] 0.0
blast CAE69454 Hypothetical protein CBG15645 [Caenorhabditis briggsae] 0.0
blast CAE69228 Hypothetical protein CBG15268 [Caenorhabditis briggsae] 0.0
Sequence
Target Sequence 
>C. ELEGANS:177031_AT
aaacggctcagaattgtgtgctcagcggcatcgaatcaccgtcaacaagtgctcgtggcc
gccaggcaaaagcatgtgctctccaacaattgtgctcctcgaacggagacgaactttggg
cttctgaagccaattcggcgtgtcgttcagcttttacatcggcattcacgtctcccgacc
cgattgtcgcgtcggcggctctccgcggagcatctcatgtgctcaaagtgtcgattgatc
gagatttgatgtcggcggtggctagatcgctaaatcacgcgtcaacagacgtccgtaaga
cggctggaattgcattgggacacgtcggacattcggcggatcttccaaatgatattctga
agcttatcgttccacagctcatcaacggttgcaaagagagcaactcacaagttcgagcag
cttcagagcttgcgttggttcatgctctca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAACGGCTCAGAATTGTGTGCTCAG 603 127 5234 Antisense
TGTGCTCAGCGGCATCGAATCACCG 461 555 5250 Antisense
ATCACCGTCAACAAGTGCTCGTGGC 23 27 5268 Antisense
TGCTCGTGGCCGCCAGGCAAAAGCA 552 579 5283 Antisense
TCTCCAACAATTGTGCTCCTCGAAC 82 611 5313 Antisense
GAAGCCAATTCGGCGTGTCGTTCAG 508 345 5359 Antisense
GTCGTTCAGCTTTTACATCGGCATT 8 473 5375 Antisense
GATGTCGGCGGTGGCTAGATCGCTA 247 407 5481 Antisense
GGAATTGCATTGGGACACGTCGGAC 321 533 5539 Antisense
TTCCACAGCTCATCAACGGTTGCAA 314 701 5603 Antisense
GAGCTTGCGTTGGTTCATGCTCTCA 373 389 5659 Antisense
Sequence Source Affymetrix Proprietary Database