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[177018_s_at]

Description Y94H6A.F /REP_DB=WormBase Gene ID /WP=CE25633 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF60892.1
Cluster 6grp_highest_10_10 > 95      6grp_high1_10_10 > 68      4grp_highest_8_8 > 30      4grp_high_8_8 > 05      6grp_highest_nonorm > 90      6grp_total > 69      4grp_total > 04      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 507
1 2 3 4 range
A 317.5
(-1.4)
P 		218.5
(-3)
P 		232
(-2.8)
P 		589.2
(3)
P 		371
B 265.3
(-1.9)
P 		174.9
(-3)
P 		220.4
(-2.5)
P 		682.1
(3)
P 		507
C 265.2
(-2)
P 		203.2
(-3)
P 		248
(-2.2)
P 		559.1
(3)
P 		356
avr 282.7
(-1.8) 		198.9
(-3) 		233.5
(-2.5) 		610.1
(3) 		411

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 217
1 2 3 4 5 6 range
A 161.6
(-0.9)
P 		154.5
(-1.2)
P 		112.9
(-3)
P 		153.2
(-1.3)
P 		181.2
(-0.1)
P 		254.9
(3)
P 		142
B 165.5
(-0.7)
P 		161.7
(-0.9)
P 		96.5
(-2.8)
P 		89.8
(-3)
P 		165.1
(-0.8)
P 		290.8
(3)
P 		201
C 174.8
(0.1)
P 		171
(-0)
P 		73.4
(-3)
P 		86.5
(-2.6)
P 		156.8
(-0.4)
P 		269.5
(3)
P 		196
avr 167.3
(-0.5) 		162.4
(-0.7) 		94.3
(-3) 		109.8
(-2.5) 		167.7
(-0.5) 		271.7
(3) 		177
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 102
1 2 3 4 range
A 115.6
(3)
P 		47.4
(-3)
P 		60.6
(-1.8)
P 		86.2
(0.4)
P 		68
B 84.6
(-0.6)
P 		43.2
(-3)
P 		51.1
(-2.5)
P 		145.4
(3)
P 		102
C 87.8
(1.9)
P 		58.4
(-3)
P 		86.4
(1.7)
P 		94.3
(3)
P 		36
avr 96
(1.7) 		49.7
(-3) 		66
(-1.3) 		108.6
(3) 		59

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 91
1 2 3 4 5 6 range
A 29.8
(-2.2)
P 		54.9
(1.8)
P 		24.6
(-3)
P 		38.9
(-0.7)
P 		54.7
(1.8)
P 		62.1
(3)
P 		38
B 57.6
(-0.6)
P 		53.8
(-0.9)
P 		23.4
(-3)
P 		23.8
(-3)
P 		67.1
(0.1)
P 		108.4
(3)
P 		85
C 64.8
(0.9)
P 		52.2
(-0.1)
P 		18.7
(-2.9)
P 		17.4
(-3)
P 		50.7
(-0.3)
P 		90.2
(3)
P 		73
avr 50.7
(-0.4) 		53.6
(-0.1) 		22.2
(-3) 		26.7
(-2.6) 		57.5
(0.3) 		86.9
(3) 		65
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 177018_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19192
Sequence Type Exemplar sequence
Representative Public ID Y94H6A.F NCBI
Target Description Y94H6A.F /REP_DB=WormBase Gene ID /WP=CE25633 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF60892.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_171295(11), NM_171294(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_171295 NCBI Caenorhabditis elegans Y94H6A.5a (Y94H6A.5) mRNA, complete cds. 11/11 A 
NM_171294 NCBI Caenorhabditis elegans Y94H6A.5b (Y94H6A.5) mRNA, complete cds. 11/11 A 
SNAP00000003279 ENSEMBL cdna:SNAP chromosome:CEL140:IV:2682584:2703967:-1 11/11 None
GENEFINDER00000003292 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:2689028:2703967:-1 9/11 None
Y94H6A.5a ENSEMBL cdna:known chromosome:CEL140:IV:2688944:2703974:-1 gene:Y94H6A.5 11/11 A 
Y94H6A.5b ENSEMBL cdna:known chromosome:CEL140:IV:2688958:2703967:-1 gene:Y94H6A.5 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:2689033-2703973(-) 96.31 100.0
Public Domain and Genome References
Ensembl Y94H6A.5
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAU05555 Hypothetical protein Y94H6A.5a [Caenorhabditis elegans] ref|NP_741348.2| Y94H6A.5a [Caenorhabditis elegans] 0.0
blast AAU05556 Hypothetical protein Y94H6A.5b [Caenorhabditis elegans] ref|NP_741347.2| Y94H6A.5b [Caenorhabditis elegans] 0.0
blast CAE68235 Hypothetical protein CBG13909 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001650 EMBL-EBI Helicase, C-terminal 9.9E-25
Pfam IPR001410 EMBL-EBI DEAD/DEAH box helicase 1.1E-83
Pfam IPR001650 EMBL-EBI Helicase, C-terminal 9.9E-25
Pfam IPR001410 EMBL-EBI DEAD/DEAH box helicase 4.6E-72
Pfam IPR007087 EMBL-EBI Zn-finger, C2H2 type 1.5E-7
Pfam IPR007087 EMBL-EBI Zn-finger, C2H2 type 1.9E-5
Pfam IPR001650 EMBL-EBI Helicase, C-terminal 9.9E-25
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_741347.2 1 848-870
Y94H6A.5b 1 848-870
Sequence
Target Sequence 
>C. ELEGANS:177018_S_AT
ggaacagattcttaagggtcgccgtaagcaagagcaactcgaaaactacatggagcatcg
ccgtcagtcgaatctcaaaaagaaggccgagcatcttggacgaggtggagccggcggtgc
tcgtggaggagcccgtggaggagctcgtggcggagctcgtggaggaagctcccgaggggc
tcgcggaggagccc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGAACAGATTCTTAAGGGTCGCCGT 486 527 2415 Antisense
GGGTCGCCGTAAGCAAGAGCAACTC 571 491 2430 Antisense
CAAGAGCAACTCGAAAACTACATGG 386 183 2443 Antisense
AAAACTACATGGAGCATCGCCGTCA 345 129 2456 Antisense
CATCGCCGTCAGTCGAATCTCAAAA 11 211 2470 Antisense
CGCCGTCAGTCGAATCTCAAAAAGA 71 257 2473 Antisense
AAGAAGGCCGAGCATCTTGGACGAG 588 151 2494 Antisense
GCCGAGCATCTTGGACGAGGTGGAG 17 283 2500 Antisense
CCCGTGGAGGAGCTCGTGGCGGAGC 522 259 2546 Antisense
GGAGCTCGTGGCGGAGCTCGTGGAG 482 521 2554 Antisense
TCCCGAGGGGCTCGCGGAGGAGCCC 12 595 2584 Antisense
Sequence Source Affymetrix Proprietary Database