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[177015_at]

Description Y34B4A.F /REP_DB=WormBase Gene ID /WP=CE21522 /CHR=X /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF59641.1
Cluster 6grp_highest_10_10 > 34      6grp_high1_10_10 > 41      4grp_highest_8_8 > 56      4grp_high_8_8 > 34      6grp_highest_nonorm > 81      6grp_total > 40      4grp_total > 37      6grp_highest_sub > 02_03      6grp_high1_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 127
1 2 3 4 range
A 320.1
(3)
P 		289.9
(-0.5)
P 		267.6
(-3)
P 		268.7
(-2.9)
P 		53
B 346.1
(3)
P 		305.1
(1.1)
P 		218.8
(-3)
P 		263.7
(-0.9)
P 		127
C 323.6
(3)
P 		299.9
(0.9)
P 		254.9
(-3)
P 		273.8
(-1.3)
P 		69
avr 329.9
(3) 		298.3
(0.7) 		247.1
(-3) 		268.7
(-1.4) 		83

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 133
1 2 3 4 5 6 range
A 281.8
(-0.9)
P 		357
(3)
P 		305.9
(0.3)
P 		316.3
(0.9)
P 		242.7
(-3)
P 		272.8
(-1.4)
P 		114
B 295.2
(-1.1)
P 		292.8
(-1.3)
P 		352.4
(3)
P 		270.5
(-2.9)
P 		282.3
(-2.1)
P 		269.2
(-3)
P 		83
C 276
(-0.2)
P 		327.5
(2.5)
P 		337.5
(3)
P 		325.1
(2.3)
P 		223.8
(-3)
P 		245.2
(-1.9)
P 		114
avr 284.3
(-0.5) 		325.8
(2.6) 		331.9
(3) 		304
(1) 		249.6
(-3) 		262.4
(-2.1) 		82
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 77
1 2 3 4 range
A 116.5
(3)
P 		62.9
(-1.2)
P 		70
(-0.6)
P 		39.3
(-3)
P 		77
B 110.4
(3)
P 		75.3
(-0.5)
P 		50.7
(-3)
P 		56.2
(-2.4)
P 		60
C 107.1
(3)
P 		86.2
(0.9)
P 		88.8
(1.2)
P 		46.2
(-3)
P 		61
avr 111.3
(3) 		74.8
(-0.4) 		69.8
(-0.9) 		47.2
(-3) 		64

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 75
1 2 3 4 5 6 range
A 52
(-3)
P 		127
(3)
P 		66.6
(-1.8)
P 		80.2
(-0.7)
P 		73.2
(-1.3)
P 		66.4
(-1.8)
P 		75
B 102.7
(1.3)
P 		97.4
(0.6)
P 		85.6
(-1.1)
P 		71.6
(-3)
P 		114.7
(3)
P 		100.4
(1)
P 		43
C 102.3
(3)
P 		99.9
(2.6)
P 		85.9
(0.3)
P 		65.4
(-3)
P 		72.4
(-1.9)
P 		82.1
(-0.3)
P 		37
avr 85.7
(-0.8) 		108.1
(3) 		79.4
(-1.8) 		72.4
(-3) 		86.8
(-0.6) 		83
(-1.2) 		36
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177015_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19556
Sequence Type Exemplar sequence
Representative Public ID Y34B4A.F NCBI
Target Description Y34B4A.F /REP_DB=WormBase Gene ID /WP=CE21522 /CHR=X /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF59641.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076495(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076495 NCBI Caenorhabditis elegans Y34B4A.2 (Y34B4A.2) mRNA, complete cds. 11/11 None
GENEFINDER00000022892 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:5279588:5284535:1 11/11 None
Y34B4A.2 ENSEMBL cdna:known chromosome:CEL140:X:5279588:5283367:1 gene:Y34B4A.2 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000022886 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:5279588-5283368(+) 90.05 100.0
Public Domain and Genome References
Gene Title
Ensembl Y34B4A.2
Entrez Gene 189585 Entrez gene
SwissProt Q9N465 EMBL-EBI
WormBase CE21522 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAF59641 Hypothetical protein Y34B4A.2 [Caenorhabditis elegans] ref|NP_508896.1| Y34B4A.2 [Caenorhabditis elegans] 1.0E-174
blast AAF59641 Hypothetical protein Y34B4A.2 [Caenorhabditis elegans] ref|NP_508896.1| Y34B4A.2 [Caenorhabditis elegans] 1.0E-168
blast CAE68726 Hypothetical protein CBG14652 [Caenorhabditis briggsae] 1.0E-131
blast CAE68726 Hypothetical protein CBG14652 [Caenorhabditis briggsae] 1.0E-117
blast CAB03420 Hypothetical protein T23G11.7a [Caenorhabditis elegans] ref|NP_492138.1| T23G11.7a [Caenorhabditis elegans] 3.0E-56
blast CAB03420 Hypothetical protein T23G11.7a [Caenorhabditis elegans] ref|NP_492138.1| T23G11.7a [Caenorhabditis elegans] 1.0E-50
Protein Domains
Database ID Description E-Value
scop a.2.7.Seryl-tRNA synthetase (SerRS) All alpha proteins; Long alpha-hairpin; tRNA-binding arm; Seryl-tRNA synthetase (SerRS) 6.40000009536743
Sequence
Target Sequence 
>C. ELEGANS:177015_AT
gctggtcgcctatcggaaaacgtttcttttacaggagatcatggatgttttcaaaattga
tattgacggtggcccagcttccatgaaacaaaaccgagtgcggagtgattgcaaatgtgt
gctcgtcgacacaatacgaggacttcacctaccacatattgcctcaattcaactttcgcc
acacaacgaacgggaaacaactgcagcaattggactgctcattcaaatgctacatgtcat
cagtcgcgttttggaatactccttgcgttatccgattgtgcctgcagcatctttttctaa
agtttactgtccgatagatgacaaatgggcaacgctatccggatggaagaaaagatcgga
acgtgaacgatttcttgaaggactcagttggctagccaaaaatattgcccaacttcgatc
cgactgcggaattcctactccaatggctgata

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCTGGTCGCCTATCGGAAAACGTTT 485 239 420 Antisense
TTGATATTGACGGTGGCCCAGCTTC 297 703 476 Antisense
CAATACGAGGACTTCACCTACCACA 678 179 551 Antisense
TCAATTCAACTTTCGCCACACAACG 470 633 583 Antisense
AATGCTACATGTCATCAGTCGCGTT 279 165 645 Antisense
AGTCGCGTTTTGGAATACTCCTTGC 300 61 661 Antisense
TACTCCTTGCGTTATCCGATTGTGC 71 647 676 Antisense
TTGTGCCTGCAGCATCTTTTTCTAA 59 707 695 Antisense
AATGGGCAACGCTATCCGGATGGAA 513 163 743 Antisense
AAAATATTGCCCAACTTCGATCCGA 629 133 818 Antisense
GGAATTCCTACTCCAATGGCTGATA 125 535 847 Antisense
Sequence Source Affymetrix Proprietary Database