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[177014_at]

Description Y55D5A.E /REP_DB=WormBase Gene ID /WP=CE22516 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAG23454.1
Cluster 6grp_highest_10_10 > 05      6grp_high1_10_10 > 50      4grp_high_8_8 > 43      6grp_highest_nonorm > 61      6grp_total > 51      4grp_total > 52      6grp_highest_sub > 03_04      6grp_high1_sub > 03_04      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 422
1 2 3 4 range
A 572.2
(-1.4)
P 		783.6
(3)
P 		693.2
(1.1)
P 		492.1
(-3)
P 		292
B 774.4
(3)
P 		721.5
(2.2)
P 		655.6
(1.3)
P 		361.3
(-3)
A 		413
C 719.3
(2)
P 		783.4
(3)
P 		523.7
(-1.1)
P 		405.5
(-3)
P 		378
avr 688.6
(1.7) 		762.8
(3) 		624.2
(0.6) 		419.6
(-3) 		343

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 341
1 2 3 4 5 6 range
A 376.7
(-1)
P 		432
(0.4)
P 		472.6
(1.4)
P 		533.4
(3)
P 		300.1
(-3)
P 		298.2
(-3)
P 		235
B 372.3
(-0.1)
P 		386
(0.1)
P 		549.5
(3)
P 		441.2
(1.1)
P 		398.7
(0.3)
P 		208.6
(-3)
P 		341
C 419.8
(1.2)
P 		426.8
(1.3)
P 		493.8
(2.7)
P 		507.2
(3)
P 		284.1
(-1.7)
P 		220.2
(-3)
P 		287
avr 389.6
(0.4) 		414.9
(0.9) 		505.3
(3) 		493.9
(2.7) 		327.6
(-1.1) 		242.3
(-3) 		263
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 179
1 2 3 4 range
A 208.3
(3)
P 		170.1
(1.3)
P 		181.2
(1.8)
P 		72
(-3)
P 		136
B 247
(3)
P 		178.2
(0.6)
P 		151.9
(-0.4)
P 		77
(-3)
A 		170
C 238.1
(3)
P 		225.2
(2.5)
P 		182.5
(1)
P 		68.4
(-3)
P 		170
avr 231.1
(3) 		191.2
(1.5) 		171.9
(0.8) 		72.5
(-3) 		159

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 93
1 2 3 4 5 6 range
A 69.4
(-3)
P 		153.6
(3)
P 		102.9
(-0.6)
P 		135.3
(1.7)
P 		90.6
(-1.5)
P 		72.6
(-2.8)
P 		84
B 129.5
(0.7)
P 		128.5
(0.6)
P 		133.4
(1)
P 		116.9
(-0.2)
P 		162
(3)
P 		77.8
(-3)
P 		84
C 155.6
(3)
P 		130.2
(1.1)
P 		125.7
(0.8)
P 		102
(-0.9)
P 		91.9
(-1.7)
P 		73.7
(-3)
P 		82
avr 118.2
(1.2) 		137.4
(3) 		120.7
(1.4) 		118.1
(1.2) 		114.8
(0.8) 		74.7
(-3) 		63
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177014_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18931
Sequence Type Exemplar sequence
Representative Public ID Y55D5A.E NCBI
Target Description Y55D5A.E /REP_DB=WormBase Gene ID /WP=CE22516 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAG23454.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065248(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065248 NCBI Caenorhabditis elegans Y55D5A.4 (Y55D5A.4) mRNA, complete cds. 11/11 None
GENEFINDER00000010559 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:2988268:2990046:-1 11/11 None
Y55D5A.4 ENSEMBL cdna:known chromosome:CEL140:III:2988268:2990046:-1 gene:Y55D5A.4 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000010552 2/11 Cross Hyb Matching Probes None
SNAP00000010553 7/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:2988267-2990046(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y55D5A.4
Entrez Gene 175409 Entrez gene
SwissProt Q9GUH8 EMBL-EBI
WormBase CE22516 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK29948 Hypothetical protein Y55D5A.4 [Caenorhabditis elegans] ref|NP_497649.1| Y55D5A.4 [Caenorhabditis elegans] 5.0E-93
blast CAE57499 Hypothetical protein CBG00471 [Caenorhabditis briggsae] 5.0E-91
Sequence
Target Sequence 
>C. ELEGANS:177014_AT
tcaatgaggcgacacgtacccgccgtcacaaatttgcgctgaaatatgatttgaacaata
atactgagcaaaaagagctcgaggctcaagaatgcccatcgggtgccggaatgagcgaga
cttttgtctgggattggccgttttctagtgatggaaccgccccgggagcctacacccctg
aaaagttccttgtctgtctcccattcaacaaaggaggaactggagagaaaaatggaacta
caaaaaagttcgagagaattgccgagtcggatttttcctggagcatgggtttgtcacgtg
acgccgagctgggctccacgtgtttttggagactcgagatcgaaattatccggaatatga
atgctgtcgtgctgattgcttcgagagatatcagccgatacaacgagaatacaaagcgtc
tgaagcgtgtcagaaaaatgtatcgcctgccggact

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCAATGAGGCGACACGTACCCGCCG 637 631 44 Antisense
TACCCGCCGTCACAAATTTGCGCTG 459 639 60 Antisense
GCTCGAGGCTCAAGAATGCCCATCG 219 299 120 Antisense
ATGCCCATCGGGTGCCGGAATGAGC 655 39 135 Antisense
TTGTCTGGGATTGGCCGTTTTCTAG 690 705 167 Antisense
ACACCCCTGAAAAGTTCCTTGTCTG 618 107 215 Antisense
TTGTCTGTCTCCCATTCAACAAAGG 637 703 233 Antisense
GCCGAGTCGGATTTTTCCTGGAGCA 303 285 304 Antisense
GAGCATGGGTTTGTCACGTGACGCC 305 389 324 Antisense
TGAATGCTGTCGTGCTGATTGCTTC 462 575 401 Antisense
AGAAAAATGTATCGCCTGCCGGACT 668 71 475 Antisense
Sequence Source Affymetrix Proprietary Database