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[177005_at]

Description Y94H6A.G /REP_DB=WormBase Gene ID /WP=CE25634 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF60893.1
Cluster 6grp_total > 74      4grp_total > 42      6grp_rule_based > S4      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 99
1 2 3 4 range
A 5.5
(-3)
A 		103
(3)
A 		28.4
(-1.6)
A 		32.3
(-1.4)
A 		98
B 4.9
(-2.9)
A 		34.6
(3)
A 		13.6
(-1.2)
A 		4.6
(-3)
A 		30
C 3.9
(-3)
A 		51.6
(3)
A 		43.6
(2)
A 		10.7
(-2.1)
A 		48
avr 4.8
(-3) 		63.1
(3) 		28.5
(-0.6) 		15.9
(-1.9) 		58

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 96
1 2 3 4 5 6 range
A 16.6
(-3)
A 		26.8
(-2.3)
A 		32.8
(-1.8)
A 		98.3
(3)
P 		60.6
(0.2)
A 		51.8
(-0.4)
A 		82
B 9.9
(-2.9)
A 		8.3
(-3)
A 		85.3
(1.8)
P 		104.6
(3)
P 		48.8
(-0.5)
A 		61.3
(0.3)
A 		96
C 34.6
(-3)
A 		35.3
(-2.9)
A 		60.5
(-0.2)
A 		91
(3)
A 		82.8
(2.1)
A 		62.4
(-0)
M 		56
avr 20.4
(-3) 		23.5
(-2.8) 		59.5
(0) 		98
(3) 		64.1
(0.4) 		58.5
(-0.1) 		78
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 21
1 2 3 4 range
A 2
(-3)
A 		22.4
(3)
A 		7.4
(-1.4)
A 		4.7
(-2.2)
A 		20
B 1.6
(-2.5)
A 		8.6
(3)
A 		3.2
(-1.3)
A 		1
(-3)
A 		8
C 1.3
(-3)
A 		14.8
(2.8)
A 		15.2
(3)
A 		1.8
(-2.8)
A 		14
avr 1.6
(-3) 		15.3
(3) 		8.6
(0.1) 		2.5
(-2.6) 		14

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 25
1 2 3 4 5 6 range
A 3.1
(-3)
A 		9.5
(-1.2)
A 		7.2
(-1.9)
A 		24.9
(3)
P 		18.3
(1.2)
A 		12.6
(-0.4)
A 		22
B 3.4
(-2.9)
A 		2.8
(-3)
A 		20.7
(1.3)
P 		27.7
(3)
P 		19.8
(1.1)
A 		22.9
(1.8)
A 		25
C 12.8
(-2.3)
A 		10.8
(-3)
A 		15.4
(-1.3)
A 		18.3
(-0.2)
A 		26.8
(3)
A 		20.9
(0.8)
M 		16
avr 6.4
(-3) 		7.7
(-2.5) 		14.4
(-0.2) 		23.6
(3) 		21.6
(2.3) 		18.8
(1.3) 		17
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177005_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19310
Sequence Type Exemplar sequence
Representative Public ID Y94H6A.G NCBI
Target Description Y94H6A.G /REP_DB=WormBase Gene ID /WP=CE25634 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF60893.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067841(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067841 NCBI Caenorhabditis elegans Y94H6A.4 (Y94H6A.4) mRNA, complete cds. 9/11 None
Y94H6A.4 ENSEMBL cdna:known chromosome:CEL140:IV:2673095:2674731:-1 gene:Y94H6A.4 9/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000003275 3/11 Cross Hyb Matching Probes None
GENEFINDER00000003294 5/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:2673075-2674737(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y94H6A.4
Entrez Gene 190801 Entrez gene
SwissProt Q9N2X2 EMBL-EBI
WormBase CE34620 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6979 response to oxidative stress inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4602 glutathione peroxidase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAF60893 Hypothetical protein Y94H6A.4 [Caenorhabditis elegans] ref|NP_500242.2| Y94H6A.4 [Caenorhabditis elegans] 7.0E-69
blast CAB02655 Hypothetical protein C11E4.2 [Caenorhabditis elegans] ref|NP_509616.1| C11E4.2 [Caenorhabditis elegans] sp|Q95003|GPX3_CAEEL Glutathione peroxidase precursor 1.0E-51
blast CAB02659 Hypothetical protein C11E4.1 [Caenorhabditis elegans] ref|NP_509615.2| C11E4.1 [Caenorhabditis elegans] 2.0E-45
Protein Domains
Database ID Description E-Value
Pfam IPR000889 EMBL-EBI Glutathione peroxidase 1.4E-43
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_500242.2 1 5-22
Y94H6A.4 1 5-22
Sequence
Target Sequence 
>C. ELEGANS:177005_AT
gaattccctacttactttggctgtttctttcacagtaattctctttgcgttttgtgtcga
agtggacgatactctgcgctggaaacaatgtgcagtcacgaatcaaagtgtattcgattt
tcaaattgaaaccttaaaaggagattacacggatcttagtcaatatcgaggaaaggttac
acttctggtcaatgttgctactttctgtgcctacactcaacagtacaccgatttcaaccc
catactcgacaaatatcaaaaacaaggcctagtcatcgccgcatttccatgcaatcagtt
ctatttacaagaacctgcagagaatcacgaattattgaatggcctgactcatgaatcctg
tccacaaactgtcgagaaaatcggaaagagtgaggaattgatgtacaacccgattcgagc
atctgatatcatttggaactttgagaaattccttatcgatagaaatggagttccgagatt
ccgtttccat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAATTCCCTACTTACTTTGGCTGTT 137 323 21 Antisense
AGTAATTCTCTTTGCGTTTTGTGTC 40 63 54 Antisense
GTCGAAGTGGACGATACTCTGCGCT 701 473 76 Antisense
ACTCAACAGTACACCGATTTCAACC 703 99 235 Antisense
ATTTCAACCCCATACTCGACAAATA 378 13 251 Antisense
AAAACAAGGCCTAGTCATCGCCGCA 191 131 279 Antisense
TCGCCGCATTTCCATGCAATCAGTT 621 599 296 Antisense
GAATGGCCTGACTCATGAATCCTGT 497 333 357 Antisense
TGAATCCTGTCCACAAACTGTCGAG 589 575 372 Antisense
ATGTACAACCCGATTCGAGCATCTG 513 47 421 Antisense
TGGAGTTCCGAGATTCCGTTTCCAT 278 549 486 Antisense
Sequence Source Affymetrix Proprietary Database