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[177003_at]

Description Y67D8C.A /REP_DB=WormBase Gene ID /WP=CE26230 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAG23369.1
Cluster 6grp_highest_10_10 > 52      6grp_high1_10_10 > 26      4grp_highest_8_8 > 58      4grp_high_8_8 > 33      6grp_highest_nonorm > 70      6grp_total > 25      4grp_total > 46      6grp_highest_sub > 01_02      6grp_cgc > 24      6grp_high1_sub > De      6grp_cgc_sub > Cu      6grp_total_sub > Decrease      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 377
1 2 3 4 range
A 532.9
(3)
P 		269
(-2.8)
P 		273.4
(-2.8)
P 		262.2
(-3)
P 		271
B 561.5
(3)
P 		317.6
(-0.9)
P 		184.4
(-3)
P 		218.2
(-2.5)
P 		377
C 458
(3)
P 		318
(-0.2)
P 		257.7
(-1.6)
P 		198.8
(-3)
P 		259
avr 517.5
(3) 		301.5
(-1.5) 		238.5
(-2.8) 		226.4
(-3) 		291

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 336
1 2 3 4 5 6 range
A 323.1
(3)
P 		302.4
(2.4)
P 		270.8
(1.4)
P 		181.7
(-1.3)
P 		124.1
(-3)
P 		190.4
(-1)
P 		199
B 459.7
(3)
P 		385.9
(1.6)
P 		152.2
(-3)
P 		173.3
(-2.6)
P 		153.5
(-3)
P 		216.8
(-1.7)
P 		308
C 271.1
(0.8)
P 		338.1
(3)
P 		230
(-0.6)
P 		219
(-1)
P 		157.9
(-3)
P 		171.7
(-2.5)
P 		180
avr 351.3
(3) 		342.1
(2.7) 		217.7
(-0.9) 		191.3
(-1.7) 		145.2
(-3) 		193
(-1.6) 		206
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 161
1 2 3 4 range
A 194
(3)
P 		58.4
(-2.2)
P 		71.5
(-1.7)
P 		38.4
(-3)
P 		156
B 179.1
(3)
P 		78.4
(-1.4)
P 		42.7
(-3)
P 		46.5
(-2.8)
P 		136
C 151.6
(3)
P 		91.4
(-0.1)
P 		89.8
(-0.1)
P 		33.5
(-3)
P 		118
avr 174.9
(3) 		76.1
(-1.4) 		68
(-1.7) 		39.5
(-3) 		135

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 123
1 2 3 4 5 6 range
A 59.6
(-1.1)
P 		107.5
(3)
P 		59
(-1.2)
P 		46.1
(-2.3)
P 		37.4
(-3)
P 		46.4
(-2.2)
P 		70
B 160
(3)
P 		128.5
(1.5)
P 		37
(-3)
P 		45.9
(-2.6)
P 		62.4
(-1.8)
P 		80.8
(-0.9)
P 		123
C 100.5
(2.7)
P 		103.2
(3)
P 		58.6
(-1.5)
P 		44
(-3)
P 		51.1
(-2.3)
P 		57.5
(-1.6)
P 		59
avr 106.7
(2.4) 		113.1
(3) 		51.5
(-2.5) 		45.3
(-3) 		50.3
(-2.6) 		61.6
(-1.6) 		68
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177003_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19255
Sequence Type Exemplar sequence
Representative Public ID Y67D8C.A NCBI
Target Description Y67D8C.A /REP_DB=WormBase Gene ID /WP=CE26230 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAG23369.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_171299(11), NM_067893(11), NM_001028385(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_171299 NCBI Caenorhabditis elegans Membrane Calcium ATPase family member (mca-3) (mca-3) mRNA, complete cds. 11/11 None
NM_067893 NCBI Caenorhabditis elegans Membrane Calcium ATPase family member (mca-3) (mca-3) mRNA, complete cds. 11/11 A 
NM_001028385 NCBI Caenorhabditis elegans Membrane Calcium ATPase family member (mca-3) (mca-3) mRNA, complete cds. 11/11 None
SNAP00000001622 ENSEMBL cdna:SNAP chromosome:CEL140:IV:3124012:3131366:-1 11/11 None
GENEFINDER00000001631 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:3116353:3131366:-1 11/11 None
Y67D8C.10c ENSEMBL cdna:known chromosome:CEL140:IV:3103163:3131366:-1 gene:Y67D8C.10 11/11 A 
Y67D8C.10a ENSEMBL cdna:known chromosome:CEL140:IV:3103817:3131366:-1 gene:Y67D8C.10 11/11 A 
Y67D8C.10b ENSEMBL cdna:known chromosome:CEL140:IV:3111405:3131366:-1 gene:Y67D8C.10 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:3124017-3131372(-) 100.0 100.0
Public Domain and Genome References
Ensembl Y67D8C.10
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAM97979 Membrane calcium atpase protein 3, isoform c [Caenorhabditis elegans] ref|NP_741352.1| Membrane Calcium ATPase family member (mca-3) [Caenorhabditis elegans] 0.0
blast AAK68550 Membrane calcium atpase protein 3, isoform a [Caenorhabditis elegans] ref|NP_500294.2| Membrane Calcium ATPase family member (mca-3) [Caenorhabditis elegans] 0.0
blast CAE59888 Hypothetical protein CBG03371 [Caenorhabditis briggsae] 0.0
blast AAK68551 Membrane calcium atpase protein 3, isoform b [Caenorhabditis elegans] ref|NP_001023556.1| Membrane Calcium ATPase family member (mca-3) [Caenorhabditis elegans] 0.0
blast CAA09303 calcium ATPase [Caenorhabditis elegans] 0.0
Protein Families
Method ID Description E-Value
ec A1A3_HUMAN (P13637) Sodium/potassium-transporting ATPase alpha-3 chain (EC 3.6.3.9) (Sodium pump 3) (Na+/K+ ATPase 3) (Alpha(III)) 1.0E-47
ec A1A2_HUMAN (P50993) Sodium/potassium-transporting ATPase alpha-2 chain precursor (EC 3.6.3.9) (Sodium pump 2) (Na+/K+ ATPase 2) 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR006068 EMBL-EBI Cation transporting ATPase, C-terminal 4.5E-18
Pfam IPR008250 EMBL-EBI E1-E2 ATPase-associated region 2.7E-21
Pfam IPR008250 EMBL-EBI E1-E2 ATPase-associated region 2.6E-18
Pfam IPR005834 EMBL-EBI Haloacid dehalogenase-like hydrolase 1.2E-12
Pfam IPR004014 EMBL-EBI Cation transporting ATPase, N-terminal 1.2E-22
Pfam IPR006068 EMBL-EBI Cation transporting ATPase, C-terminal 9.1E-18
Pfam IPR008250 EMBL-EBI E1-E2 ATPase-associated region 8.2E-17
Pfam IPR008250 EMBL-EBI E1-E2 ATPase-associated region 2.6E-18
Pfam IPR005834 EMBL-EBI Haloacid dehalogenase-like hydrolase 1.2E-12
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_741352.1 10 86-108,123-142,334-356,376-398,823-845,855-872,904-926,941-958,970-992,1007-1029
NP_500294.2 10 86-108,123-142,334-356,376-398,823-845,855-872,904-926,941-958,970-992,1007-1029
NP_001023556.1 10 86-108,123-142,334-356,376-398,823-845,855-872,904-926,941-958,970-992,1007-1029
Y67D8C.10c 10 86-108,123-142,334-356,376-398,823-845,855-872,904-926,941-958,970-992,1007-1029
Y67D8C.10a 10 86-108,123-142,334-356,376-398,823-845,855-872,904-926,941-958,970-992,1007-1029
Y67D8C.10b 10 86-108,123-142,334-356,376-398,823-845,855-872,904-926,941-958,970-992,1007-1029
SNAP00000001622 2 86-108,123-142
GENEFINDER00000001631 10 86-108,121-143,476-498,518-540,965-987,1046-1068,1083-1100,1112-1134,1149-1171,1301-1323
Sequence
Target Sequence 
>C. ELEGANS:177003_AT
tcaggacgtcactcttgttattcttcttgtttctgcaatcgtatcacttgctctttcatt
ctacagacctcctggagaagatactgcgggttcggatgacagtgaacacgatgccggatg
gatcgaaggtgtcgcaattctcatctcagttattgtcgtcgtactggtcacggctctcaa
tgattacacaaaggaacgtcaattccgtggtctccaagcgaaaatcgaaaccgaacatcg
attcttcgtccttcgtggcggtcaatcggtacaagtcgtcgtgaatgagctcgtcgtcgg
tgacattgcccagatcaagtacggagatttgattccatcagacggaattatcattcaatc
gaatgatcttaaaatggacgagtcctcgttgacaggagaatcggatcaaatcaggaagag
ccccgagcacgatccgattattttg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCAGGACGTCACTCTTGTTATTCTT 478 621 261 Antisense
TATTCTTCTTGTTTCTGCAATCGTA 651 663 279 Antisense
TCTTTCATTCTACAGACCTCCTGGA 561 615 312 Antisense
TCGAAGGTGTCGCAATTCTCATCTC 487 601 383 Antisense
TTCTCATCTCAGTTATTGTCGTCGT 115 691 398 Antisense
ACTGGTCACGGCTCTCAATGATTAC 496 97 423 Antisense
TCAATTCCGTGGTCTCCAAGCGAAA 606 633 459 Antisense
AACCGAACATCGATTCTTCGTCCTT 316 147 489 Antisense
CCTTCGTGGCGGTCAATCGGTACAA 417 267 510 Antisense
GTCGGTGACATTGCCCAGATCAAGT 41 473 556 Antisense
CCCCGAGCACGATCCGATTATTTTG 490 259 681 Antisense
Sequence Source Affymetrix Proprietary Database