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[177000_at]

Description Y54G2A.H /REP_DB=WormBase Gene ID /WP=CE25450 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF59568.1
Cluster 6grp_high1_10_10 > 59      4grp_highest_8_8 > 63      4grp_high_8_8 > 27      6grp_total > 38      4grp_total > 29      6grp_high1_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 154
1 2 3 4 range
A 261.1
(1.4)
P 		283.8
(3)
P 		199.8
(-3)
P 		259.8
(1.3)
P 		84
B 293
(1.5)
P 		215.9
(-2.6)
P 		208.3
(-3)
P 		321.5
(3)
P 		113
C 351.4
(3)
P 		323.2
(1.9)
P 		197.5
(-3)
P 		283.6
(0.4)
P 		154
avr 301.8
(3) 		274.3
(1.3) 		201.9
(-3) 		288.3
(2.2) 		100

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 247
1 2 3 4 5 6 range
A 133.1
(-2.3)
P 		200.7
(0.9)
P 		117.6
(-3)
P 		123.6
(-2.7)
P 		172.6
(-0.4)
P 		246
(3)
P 		128
B 278.1
(3)
P 		192.8
(0.3)
P 		91.4
(-3)
P 		158.5
(-0.8)
P 		178.8
(-0.2)
P 		154.5
(-1)
A 		187
C 338.6
(3)
P 		216.3
(-0.9)
P 		167.3
(-2.4)
P 		149.6
(-3)
P 		222.8
(-0.7)
P 		188.1
(-1.8)
A 		189
avr 249.9
(3) 		203.3
(0.8) 		125.4
(-3) 		143.9
(-2.1) 		191.4
(0.2) 		196.2
(0.4) 		125
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 78
1 2 3 4 range
A 95.1
(3)
P 		61.6
(-0.5)
P 		52.2
(-1.5)
P 		38
(-3)
P 		57
B 93.5
(3)
P 		53.3
(-2.3)
P 		48.3
(-3)
P 		68.5
(-0.3)
P 		45
C 116.3
(3)
P 		92.9
(1)
P 		68.8
(-1.2)
P 		47.8
(-3)
P 		69
avr 101.6
(3) 		69.3
(-0.9) 		56.4
(-2.4) 		51.4
(-3) 		50

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 103
1 2 3 4 5 6 range
A 24.5
(-3)
P 		71.4
(3)
P 		25.6
(-2.9)
P 		31.3
(-2.1)
P 		52.1
(0.5)
P 		59.9
(1.5)
P 		47
B 96.8
(3)
P 		64.2
(0.4)
P 		22.2
(-3)
P 		42
(-1.4)
P 		72.7
(1.1)
P 		57.6
(-0.2)
A 		75
C 125.5
(3)
P 		66
(-0.7)
P 		42.6
(-2.2)
P 		30.1
(-3)
P 		72.1
(-0.4)
P 		63
(-0.9)
A 		95
avr 82.3
(3) 		67.2
(1.3) 		30.1
(-3) 		34.5
(-2.5) 		65.6
(1.1) 		60.2
(0.5) 		52
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 177000_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19348
Sequence Type Exemplar sequence
Representative Public ID Y54G2A.H NCBI
Target Description Y54G2A.H /REP_DB=WormBase Gene ID /WP=CE25450 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF59568.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001028328(11), NM_001028329(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001028328 NCBI Caenorhabditis elegans Y54G2A.26a (Y54G2A.26) mRNA, complete cds. 11/11 None
NM_001028329 NCBI Caenorhabditis elegans Y54G2A.26b (Y54G2A.26) mRNA, complete cds. 11/11 None
SNAP00000036516 ENSEMBL cdna:SNAP chromosome:CEL140:IV:2986716:2989226:-1 11/11 None
GENEFINDER00000036613 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:2986606:3011196:-1 11/11 None
Y54G2A.26a.1 ENSEMBL cdna:known chromosome:CEL140:IV:2986094:2995570:-1 gene:Y54G2A.26 11/11 None
Y54G2A.26a.2 ENSEMBL cdna:known chromosome:CEL140:IV:2986095:2995571:-1 gene:Y54G2A.26 11/11 None
Y54G2A.26b ENSEMBL cdna:known chromosome:CEL140:IV:2986716:2992508:-1 gene:Y54G2A.26 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:2987600-2989232(-) 98.54 100.0
Public Domain and Genome References
Ensembl Y54G2A.26
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAF59568 Hypothetical protein Y54G2A.26a [Caenorhabditis elegans] ref|NP_001023499.1| Y54G2A.26a [Caenorhabditis elegans] 0.0
blast AAP13744 Hypothetical protein Y54G2A.26b [Caenorhabditis elegans] ref|NP_001023500.1| Y54G2A.26b [Caenorhabditis elegans] 0.0
blast CAE59877 Hypothetical protein CBG03357 [Caenorhabditis briggsae] 0.0
Sequence
Target Sequence 
>C. ELEGANS:177000_AT
aacatcagatcttggtggtggtggtggacaagtttttgaggaaattggagcaattggtgg
tggaatgagcaccgagtcgtcgttggatgataagtcggctgtgaagattccgaagcgagg
aaggccttgtaaggctccagggactacggcgactaataaatcgacaaagaggaaggagtc
tcaagacggagggcaagaggtggggggacctgttgaagataaagatgatcatgagaaaaa
gactgaggatgaagatgaaacgaatgaggagactgagaaaattgaagttcaacaagcgga
tcaagctgagagagaagatgatgcagcttccagtgagctaaaaccttcaattttttcaac
ttttccgttctgggaattggatttgatattattgaatattttgggtacaaaccgaagcga
aactgcaactccggaactagcaaccgacactacagctgttgccaagaagactcgctccgc
agcagctcgacgcaagaacactaagacggtcgacgacgagtttgcaggcgccacgaaaaa
gaagagaggatcagtgacggcagcgaccgccggaatcggtggtgacg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AACATCAGATCTTGGTGGTGGTGGT 199 137 420 Antisense
GAATGAGCACCGAGTCGTCGTTGGA 702 329 482 Antisense
GTCGTTGGATGATAAGTCGGCTGTG 117 473 498 Antisense
TGTAAGGCTCCAGGGACTACGGCGA 666 555 547 Antisense
AAACCGAAGCGAAACTGCAACTCCG 204 119 828 Antisense
GCAACTCCGGAACTAGCAACCGACA 338 317 844 Antisense
CAACCGACACTACAGCTGTTGCCAA 90 187 860 Antisense
AGCTGTTGCCAAGAAGACTCGCTCC 94 81 873 Antisense
GCAGCAGCTCGACGCAAGAACACTA 444 313 898 Antisense
GACGGTCGACGACGAGTTTGCAGGC 258 377 924 Antisense
GCGACCGCCGGAATCGGTGGTGACG 586 287 982 Antisense
Sequence Source Affymetrix Proprietary Database