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[176993_at]

Description Y54G2A.Y /REP_DB=WormBase Gene ID /WP=CE25467 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF59585.1
Cluster 6grp_total > 67      4grp_total > 34      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 22
1 2 3 4 range
A 1.9
(-3)
A 		2.9
(-1.4)
A 		2.1
(-2.7)
A 		5.6
(3)
A 		4
B 1.8
(-3)
A 		23.2
(3)
A 		3.8
(-2.4)
A 		2.7
(-2.7)
A 		21
C 1.6
(-3)
A 		1.7
(-3)
A 		2
(-2.8)
A 		15.1
(3)
A 		14
avr 1.8
(-3) 		9.3
(3) 		2.6
(-2.4) 		7.8
(1.8) 		8

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 45
1 2 3 4 5 6 range
A 8.7
(-2)
A 		1.6
(-3)
A 		1.5
(-3)
A 		2
(-2.9)
A 		3.6
(-2.7)
A 		46.2
(3)
A 		45
B 1.3
(-3)
A 		1.9
(-2.7)
A 		1.2
(-3)
A 		2.6
(-2.3)
A 		1.7
(-2.8)
A 		13.7
(3)
A 		13
C 2.9
(-2.9)
A 		6
(-2)
A 		8.9
(-1.1)
A 		2.8
(-2.9)
A 		2.5
(-3)
A 		23.2
(3)
A 		21
avr 4.3
(-2.6) 		3.2
(-2.8) 		3.9
(-2.7) 		2.5
(-3) 		2.6
(-3) 		27.7
(3) 		25
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 5
1 2 3 4 range
A 0.7
(1)
A 		0.6
(-1)
A 		0.5
(-3)
A 		0.8
(3)
A 		0
B 0.6
(-3)
A 		5.7
(3)
A 		0.9
(-2.6)
A 		0.6
(-3)
A 		5
C 0.5
(-3)
A 		0.5
(-3)
A 		0.7
(-2.4)
A 		2.5
(3)
A 		2
avr 0.6
(-3) 		2.3
(3) 		0.7
(-2.6) 		1.3
(-0.5) 		2

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 11
1 2 3 4 5 6 range
A 1.6
(-2.3)
A 		0.6
(-2.8)
A 		0.3
(-3)
A 		0.5
(-2.9)
A 		1.1
(-2.6)
A 		11.3
(3)
A 		11
B 0.4
(-2.9)
A 		0.6
(-2.6)
A 		0.3
(-3)
A 		0.7
(-2.5)
A 		0.7
(-2.5)
A 		5.1
(3)
A 		5
C 1.1
(-2.6)
A 		1.8
(-2)
A 		2.3
(-1.6)
A 		0.6
(-3)
A 		0.8
(-2.8)
A 		7.8
(3)
A 		7
avr 1
(-2.7) 		1
(-2.7) 		1
(-2.7) 		0.6
(-3) 		0.9
(-2.8) 		8.1
(3) 		8
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176993_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19316
Sequence Type Exemplar sequence
Representative Public ID Y54G2A.Y NCBI
Target Description Y54G2A.Y /REP_DB=WormBase Gene ID /WP=CE25467 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF59585.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067855(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067855 NCBI Caenorhabditis elegans Y54G2A.13 (Y54G2A.13) mRNA, complete cds. 11/11 None
SNAP00000036480 ENSEMBL cdna:SNAP chromosome:CEL140:IV:2812934:2817139:-1 11/11 None
GENEFINDER00000036610 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:2814565:2819207:-1 11/11 None
Y54G2A.13 ENSEMBL cdna:known chromosome:CEL140:IV:2814565:2818561:-1 gene:Y54G2A.13 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:2814570-2819183(-) 87.29 100.0
Public Domain and Genome References
Gene Title
Ensembl Y54G2A.13
Entrez Gene 190279 Entrez gene
SwissProt Q9N3C0 EMBL-EBI
WormBase CE32535 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAF59585 Hypothetical protein Y54G2A.13 [Caenorhabditis elegans] ref|NP_500256.2| Y54G2A.13 [Caenorhabditis elegans] 0.0
blast AAF59585 Hypothetical protein Y54G2A.13 [Caenorhabditis elegans] ref|NP_500256.2| Y54G2A.13 [Caenorhabditis elegans] 1.0E-145
blast AAK68556 Hypothetical protein Y69A2AR.12 [Caenorhabditis elegans] ref|NP_500224.1| Y69A2AR.12 [Caenorhabditis elegans] 2.0E-43
blast AAK68556 Hypothetical protein Y69A2AR.12 [Caenorhabditis elegans] ref|NP_500224.1| Y69A2AR.12 [Caenorhabditis elegans] 3.0E-43
blast AAK68558 Hypothetical protein Y69A2AR.14 [Caenorhabditis elegans] ref|NP_500220.1| Y69A2AR.14 [Caenorhabditis elegans] 2.0E-27
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_500256.2 2 7-29,369-391
Y54G2A.13 2 7-29,369-391
SNAP00000036480 1 246-268
GENEFINDER00000036610 2 59-81,421-443
Sequence
Target Sequence 
>C. ELEGANS:176993_AT
tgctccgaagactcaagcggctccaaaacctggtgcaaaacttcgagagactcaggcagc
tcagaagaaggaagttcagacgaagaaaaagtatccatctgaaaagagtgagaagagcga
ggctacatctccaactccaaagaacacagaaatgaagaagaagcttctggagactcaagc
caaccttcagaagatccgggcccacatgaagaagaacgctctacagcacaaatcggacac
tagaacatcgaagttcaaaaagatgggaccaatcttcattcccatggagcctgatattct
gcaggcgccggtacttcccaacgttgttctggacaaagtgtggtacgatccgacatgcaa
cgtgatgtacgatattggatccgatgtagacgatatcgagctggacacgtcgttcatggt
cgatcc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGCTCCGAAGACTCAAGCGGCTCCA 100 581 1350 Antisense
TCAAGCGGCTCCAAAACCTGGTGCA 130 631 1362 Antisense
AACTTCGAGAGACTCAGGCAGCTCA 298 139 1388 Antisense
GACTCAGGCAGCTCAGAAGAAGGAA 28 375 1398 Antisense
AGAAGAGCGAGGCTACATCTCCAAC 228 75 1460 Antisense
GCTACATCTCCAACTCCAAAGAACA 85 303 1471 Antisense
AGTGTGGTACGATCCGACATGCAAC 349 59 1686 Antisense
GATCCGACATGCAACGTGATGTACG 567 415 1696 Antisense
GGATCCGATGTAGACGATATCGAGC 467 513 1726 Antisense
GATATCGAGCTGGACACGTCGTTCA 708 425 1741 Antisense
TGGACACGTCGTTCATGGTCGATCC 313 547 1751 Antisense
Sequence Source Affymetrix Proprietary Database