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[176992_at]

Description Y105E8B.I /REP_DB=WormBase Gene ID /WP=CE25177 /CHR=1 /FEA=Sanger Annotation /DEF=(HINXTON) protein_id:CAB60852.1
Cluster 6grp_total > 76      4grp_total > 09      6grp_total_sub > Increase      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 70
1 2 3 4 range
A 35.4
(-0.9)
A 		15.2
(-3)
A 		37.3
(-0.7)
A 		73.5
(3)
A 		58
B 34.6
(-0.5)
A 		8.4
(-3)
A 		40.6
(0)
A 		71.8
(3)
A 		63
C 12.8
(-3)
A 		69.1
(2.1)
A 		29.3
(-1.5)
A 		78.5
(3)
A 		66
avr 27.6
(-3) 		30.9
(-2.6) 		35.7
(-2) 		74.6
(3) 		47

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 135
1 2 3 4 5 6 range
A 6
(-3)
A 		38.4
(-1)
A 		102.2
(3)
A 		50.4
(-0.2)
A 		65.6
(0.7)
A 		87.1
(2.1)
P 		96
B 5.5
(-3)
A 		22.3
(-2.2)
A 		33.2
(-1.7)
A 		44.3
(-1.2)
A 		68.2
(-0.1)
M 		134.7
(3)
P 		129
C 5.9
(-3)
A 		36.1
(-1.7)
A 		44.3
(-1.3)
A 		64.7
(-0.4)
P 		57
(-0.7)
A 		140.3
(3)
P 		134
avr 5.8
(-3) 		32.3
(-1.6) 		59.9
(-0.2) 		53.1
(-0.5) 		63.6
(0) 		120.7
(3) 		115
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 18
1 2 3 4 range
A 12.9
(3)
A 		3.3
(-3)
A 		9.7
(1)
A 		10.8
(1.7)
A 		10
B 11
(1)
A 		2.1
(-3)
A 		9.4
(0.3)
A 		15.3
(3)
A 		13
C 4.2
(-3)
A 		19.9
(3)
A 		10.2
(-0.7)
A 		13.2
(0.4)
A 		16
avr 9.4
(-1.7) 		8.4
(-3) 		9.8
(-1.2) 		13.1
(3) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 49
1 2 3 4 5 6 range
A 1.1
(-3)
A 		13.7
(0.6)
A 		22.3
(3)
A 		12.8
(0.3)
A 		19.8
(2.3)
A 		21.2
(2.7)
P 		21
B 1.9
(-3)
A 		7.4
(-2.3)
A 		8.1
(-2.2)
A 		11.7
(-1.8)
A 		27.7
(0.2)
M 		50.2
(3)
P 		48
C 2.2
(-3)
A 		11
(-1.8)
A 		11.3
(-1.8)
A 		13
(-1.6)
P 		18.4
(-0.8)
A 		47
(3)
P 		45
avr 1.7
(-3) 		10.7
(-1.6) 		13.9
(-1.1) 		12.5
(-1.3) 		22
(0.2) 		39.5
(3) 		38
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176992_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18813
Sequence Type Exemplar sequence
Representative Public ID Y105E8B.I NCBI
Target Description Y105E8B.I /REP_DB=WormBase Gene ID /WP=CE25177 /CHR=1 /FEA=Sanger Annotation /DEF=(HINXTON) protein_id:CAB60852.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_170940(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_170940 NCBI Caenorhabditis elegans Y105E8A.8 (Y105E8A.8) mRNA, complete cds. 9/11 A 
SNAP00000013328 ENSEMBL cdna:SNAP chromosome:CEL140:I:14409011:14420061:-1 9/11 None
GENEFINDER00000013499 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:14411053:14413767:-1 11/11 None
Y105E8A.8 ENSEMBL cdna:known chromosome:CEL140:I:14408875:14413767:-1 gene:Y105E8A.8 9/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:14411517-14413698(-) 73.19 81.27
Public Domain and Genome References
Gene Title
Ensembl Y105E8A.8
Entrez Gene 173303 Entrez gene
SwissProt Q8WQB4 EMBL-EBI
WormBase CE29846 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAD21659 Hypothetical protein Y105E8A.8 [Caenorhabditis elegans] ref|NP_740936.1| Y105E8A.8 [Caenorhabditis elegans] 0.0
blast CAE64131 Hypothetical protein CBG08747 [Caenorhabditis briggsae] 1.0E-127
blast CAD21659 Hypothetical protein Y105E8A.8 [Caenorhabditis elegans] ref|NP_740936.1| Y105E8A.8 [Caenorhabditis elegans] 1.0E-107
blast CAE64131 Hypothetical protein CBG08747 [Caenorhabditis briggsae] 2.0E-52
blast NP_659073 hypothetical protein LOC216853 [Mus musculus] gb|AAH69868.1| Wdr79 protein [Mus musculus] gb|AAH21790.1| Hypothetical protein LOC216853 [Mus musculus] emb|CAI52013.1| novel protein [Mus musculus] 1.0E-14
Sequence
Target Sequence 
>C. ELEGANS:176992_AT
ggtgctggattcttgttgggaaaactctggaaaaggggtgttctcgtcgtcaaaactgcg
tccaattcagctatttgatacggaaaatggaagtattctcggtgcttataatggaaaaga
tgctggggacaatatcactgcggcgatgagcatcggacaaagtggaagctcattgatagg
cggattcaagaacaaatttcaaatctgggatatcgaatatacaggcgacgctattcaaca
tatcaaagttttacaaattttttgcctgaaaaattattgtgacgtaagtattacagaaac
tgaaaattctgagaatgcaaaatttaaatttcccgctaaaacatttttcgcagaaaattt
aaatttcccgccaaaattttgggtcttaccacggtgggtctcaccacgacgggtctcacc
acgatgggtctcacctcgatgggtctcgccacgatgggtttcaccacgaaggaaattccc
ag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGTGCTGGATTCTTGTTGGGAAAAC 328 501 522 Antisense
TGGAAAAGGGGTGTTCTCGTCGTCA 662 545 549 Antisense
TTCTCGTCGTCAAAACTGCGTCCAA 477 693 562 Antisense
CAAAACTGCGTCCAATTCAGCTATT 106 189 572 Antisense
GGAAGTATTCTCGGTGCTTATAATG 511 531 610 Antisense
TGCTGGGGACAATATCACTGCGGCG 279 581 642 Antisense
ATATCACTGCGGCGATGAGCATCGG 514 1 653 Antisense
GGAAGCTCATTGATAGGCGGATTCA 268 531 685 Antisense
ACAGGCGACGCTATTCAACATATCA 449 107 742 Antisense
TTTCCCGCTAAAACATTTTTCGCAG 437 669 850 Antisense
GTTTCACCACGAAGGAAATTCCCAG 433 449 979 Antisense
Sequence Source Affymetrix Proprietary Database