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[176991_at]

Description Y54G2A.V /REP_DB=WormBase Gene ID /WP=CE25464 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF59582.1
Cluster 6grp_highest_10_10 > 95      6grp_high1_10_10 > 69      4grp_high_8_8 > 04      6grp_highest_nonorm > 90      6grp_total > 69      4grp_total > 05      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 554
1 2 3 4 range
A 281.4
(-1.3)
P 		157.2
(-2.7)
P 		131.2
(-3)
P 		652.7
(3)
P 		522
B 292.7
(-0.7)
P 		103.1
(-3)
P 		113.6
(-2.9)
A 		606.6
(3)
P 		504
C 284.9
(-0.6)
P 		112.6
(-2.8)
A 		98.7
(-3)
P 		568.5
(3)
P 		470
avr 286.3
(-0.9) 		124.3
(-2.9) 		114.5
(-3) 		609.3
(3) 		495

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 143
1 2 3 4 5 6 range
A 159.2
(-0)
P 		127.6
(-1.5)
P 		97.3
(-3)
P 		132.8
(-1.3)
P 		152
(-0.4)
P 		221.7
(3)
P 		124
B 190.9
(2.2)
P 		183.6
(1.8)
P 		81.2
(-3)
P 		116.8
(-1.3)
P 		168.5
(1.1)
P 		207.9
(3)
P 		127
C 141.3
(-1.6)
P 		209
(2.2)
P 		116.8
(-3)
P 		122
(-2.7)
P 		135.5
(-2)
P 		224.1
(3)
P 		107
avr 163.8
(0.3) 		173.4
(0.8) 		98.4
(-3) 		123.9
(-1.7) 		152
(-0.3) 		217.9
(3) 		120
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 104
1 2 3 4 range
A 102.4
(3)
P 		34.1
(-3)
P 		34.3
(-3)
P 		95.5
(2.4)
P 		68
B 93.4
(0.9)
P 		25.5
(-3)
P 		26.3
(-3)
A 		129.3
(3)
P 		104
C 94.3
(2.8)
P 		32.4
(-3)
A 		34.4
(-2.8)
P 		95.9
(3)
P 		64
avr 96.7
(2.2) 		30.7
(-3) 		31.7
(-2.9) 		106.9
(3) 		76

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 58
1 2 3 4 5 6 range
A 29.3
(-1.5)
P 		45.4
(1.4)
P 		21.2
(-3)
P 		33.7
(-0.7)
P 		45.9
(1.5)
P 		54
(3)
P 		33
B 66.4
(1.8)
P 		61.1
(1.3)
P 		19.7
(-3)
P 		30.9
(-1.8)
P 		68.5
(2.1)
P 		77.5
(3)
P 		58
C 52.4
(0.3)
P 		63.8
(1.7)
P 		29.7
(-2.4)
P 		24.5
(-3)
P 		43.9
(-0.7)
P 		75
(3)
P 		51
avr 49.4
(0.4) 		56.8
(1.4) 		23.5
(-3) 		29.7
(-2.2) 		52.8
(0.9) 		68.8
(3) 		45
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176991_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19304
Sequence Type Exemplar sequence
Representative Public ID Y54G2A.V NCBI
Target Description Y54G2A.V /REP_DB=WormBase Gene ID /WP=CE25464 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF59582.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067862(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067862 NCBI Caenorhabditis elegans Y54G2A.16 (Y54G2A.16) mRNA, complete cds. 11/11 None
GENEFINDER00000036600 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:2855698:2856169:-1 11/11 None
Y54G2A.16 ENSEMBL cdna:known chromosome:CEL140:IV:2855698:2857112:-1 gene:Y54G2A.16 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:2855703-2856175(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y54G2A.16
Entrez Gene 177069 Entrez gene
SwissProt Q9N3B7 EMBL-EBI
WormBase CE34412 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAF59582 Hypothetical protein Y54G2A.16 [Caenorhabditis elegans] ref|NP_500263.2| Y54G2A.16 [Caenorhabditis elegans] 2.0E-64
blast AAF59582 Hypothetical protein Y54G2A.16 [Caenorhabditis elegans] ref|NP_500263.2| Y54G2A.16 [Caenorhabditis elegans] 9.0E-56
blast CAE67934 Hypothetical protein CBG13534 [Caenorhabditis briggsae] 3.0E-53
blast CAE67934 Hypothetical protein CBG13534 [Caenorhabditis briggsae] 8.0E-47
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_500263.2 4 13-35,50-72,79-101,116-138
Y54G2A.16 4 13-35,50-72,79-101,116-138
GENEFINDER00000036600 3 10-32,39-61,76-98
Sequence
Target Sequence 
>C. ELEGANS:176991_AT
atgacgtcagacgctcaatcttttctcactactccaatcgttctctccttactacaattt
gccacatcgatgatcatgatagtagcggtgctcacaagcttccattggctcctgctccca
tttatgctctcctgtatagccaacctcctggcgcttgtcgttctctgctcgtggggtgtg
ctgcgacgtcatgagctctccaactcggtggtgccactgcttctaattggttccaccgga
atcggacttctctacctatggtttttaacaataatctccatgacgttcgtgcttattcga
gatcgaaaaattatggggtacgacgatgagttcaaca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGACGTCAGACGCTCAATCTTTTC 206 45 13 Antisense
TCTCTCCTTACTACAATTTGCCACA 68 613 54 Antisense
AATTTGCCACATCGATGATCATGAT 310 173 68 Antisense
TAGTAGCGGTGCTCACAAGCTTCCA 598 653 92 Antisense
TTATGCTCTCCTGTATAGCCAACCT 151 679 134 Antisense
GTGCCACTGCTTCTAATTGGTTCCA 568 477 223 Antisense
TAATTGGTTCCACCGGAATCGGACT 390 627 236 Antisense
TCGGACTTCTCTACCTATGGTTTTT 177 607 254 Antisense
AATAATCTCCATGACGTTCGTGCTT 1 173 282 Antisense
GACGTTCGTGCTTATTCGAGATCGA 236 379 294 Antisense
ATGGGGTACGACGATGAGTTCAACA 616 49 325 Antisense
Sequence Source Affymetrix Proprietary Database