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[176989_at]

Description Y54G2A.Z /REP_DB=WormBase Gene ID /WP=CE25468 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF59586.1
Cluster 6grp_high1_10_10 > 95      4grp_high_8_8 > 20      6grp_total > 96      4grp_total > 18      6grp_high1_sub > In      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 78
1 2 3 4 range
A 34.6
(-3)
A 		63.7
(-0.5)
P 		46.3
(-2)
A 		103.7
(3)
P 		69
B 43.6
(-0.8)
P 		50.6
(1.8)
P 		37.9
(-3)
A 		53.8
(3)
P 		16
C 29.9
(-2.5)
A 		44.1
(-0.8)
A 		25.4
(-3)
P 		77.2
(3)
P 		52
avr 36
(-3) 		52.8
(-0.6) 		36.5
(-2.9) 		78.2
(3) 		42

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 113
1 2 3 4 5 6 range
A 36.9
(-3)
A 		42.1
(-2.6)
A 		92.5
(1.1)
P 		103.4
(2)
P 		82.5
(0.4)
P 		117.3
(3)
P 		80
B 38.8
(-3)
A 		49.5
(-2.4)
P 		68.1
(-1.4)
A 		73.7
(-1.1)
P 		126.9
(1.7)
P 		150.2
(3)
P 		111
C 54.5
(-2.5)
P 		77.8
(-0.6)
P 		48.7
(-3)
A 		89.6
(0.4)
P 		113
(2.4)
P 		120.5
(3)
P 		72
avr 43.4
(-3) 		56.5
(-2.1) 		69.8
(-1.2) 		88.9
(0.2) 		107.5
(1.5) 		129.3
(3) 		86
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 6
1 2 3 4 range
A 12.6
(-2)
A 		13.8
(0.3)
P 		12.1
(-3)
A 		15.2
(3)
P 		3
B 13.9
(3)
P 		12.5
(1.4)
P 		8.8
(-3)
A 		11.5
(0.2)
P 		5
C 9.9
(-1.5)
A 		12.7
(2.6)
A 		8.9
(-3)
P 		13
(3)
P 		4
avr 12.1
(1) 		13
(2.6) 		9.9
(-3) 		13.2
(3) 		3

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 49
1 2 3 4 5 6 range
A 6.8
(-3)
A 		15
(-0.7)
A 		20.1
(0.7)
P 		26.2
(2.3)
P 		24.9
(2)
P 		28.6
(3)
P 		22
B 13.5
(-3)
A 		16.5
(-2.6)
P 		16.5
(-2.6)
A 		19.5
(-2.2)
P 		51.6
(2.4)
P 		56
(3)
P 		43
C 20.2
(-1.3)
P 		23.7
(-0.6)
P 		12.4
(-3)
A 		18
(-1.8)
P 		36.6
(2.2)
P 		40.3
(3)
P 		28
avr 13.5
(-3) 		18.4
(-2) 		16.3
(-2.4) 		21.2
(-1.4) 		37.7
(2.2) 		41.6
(3) 		28
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176989_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19212
Sequence Type Exemplar sequence
Representative Public ID Y54G2A.Z NCBI
Target Description Y54G2A.Z /REP_DB=WormBase Gene ID /WP=CE25468 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF59586.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067851(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067851 NCBI Caenorhabditis elegans Y54G2A.12 (Y54G2A.12) mRNA, complete cds. 11/11 None
SNAP00000036473 ENSEMBL cdna:SNAP chromosome:CEL140:IV:2781138:2795106:-1 9/11 None
GENEFINDER00000036587 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:2781895:2782821:-1 9/11 None
Y54G2A.12 ENSEMBL cdna:known chromosome:CEL140:IV:2781722:2782821:-1 gene:Y54G2A.12 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:2781727-2782827(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y54G2A.12
Entrez Gene 190278 Entrez gene
SwissProt Q9N3C1 EMBL-EBI
WormBase CE25468 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAF59586 Hypothetical protein Y54G2A.12 [Caenorhabditis elegans] ref|NP_500252.1| Y54G2A.12 [Caenorhabditis elegans] 1.0E-163
blast AAF59586 Hypothetical protein Y54G2A.12 [Caenorhabditis elegans] ref|NP_500252.1| Y54G2A.12 [Caenorhabditis elegans] 1.0E-139
blast AAF59586 Hypothetical protein Y54G2A.12 [Caenorhabditis elegans] ref|NP_500252.1| Y54G2A.12 [Caenorhabditis elegans] 1.0E-128
blast AAB96707 Yeast prp (splicing factor) related protein 17 [Caenorhabditis elegans] ref|NP_492851.1| yeast PRP (splicing factor) related family member (prp-17) [Caenorhabditis elegans] 1.0E-85
blast AAB96707 Yeast prp (splicing factor) related protein 17 [Caenorhabditis elegans] ref|NP_492851.1| yeast PRP (splicing factor) related family member (prp-17) [Caenorhabditis elegans] 2.0E-85
blast CAE73038 Hypothetical protein CBG20408 [Caenorhabditis briggsae] 4.0E-69
blast CAE73038 Hypothetical protein CBG20408 [Caenorhabditis briggsae] 5.0E-69
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000036587 1 249-271
Sequence
Target Sequence 
>C. ELEGANS:176989_AT
gcaattcggaacaactcgcttcgtgggagacgtcaaaaaagcagaagccgagaaaggagt
ttcactttttgaatcaaaaaagactggtggagagcagagaaaacgcgttggaaatgatga
ttcggcagatattgatggatatagtggtccatggagtcgatatgttgatgaaaagactgt
cgcaaaaccaacaccagaacttcagaaacaaatggatgaaattgtgaagaagggtcaaga
aaagagtagaagattgaagaaagagaaggaggatagtgagcatatggctgaagagagctc
cacgcttcattgtacgagtttattcttgattatttggctcaaaggagcccaaaaagccaa
gaagaccgctttagaattcaggaaaaatgcaaaattaagctgcaatttgtcgaactatgc
gatttcggcagattttaatcaaatttgtgtactgaaaatttacctaaaacgacacaaaac
aacaaaaattcttttaattttactatattcaactactttacgaaatattaaacacatttt
caaagattttagggaaatatcgctcacttcttcccaaaactataagccatt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCAATTCGGAACAACTCGCTTCGTG 126 323 261 Antisense
CAACTCGCTTCGTGGGAGACGTCAA 316 185 272 Antisense
GTGGTCCATGGAGTCGATATGTTGA 176 489 404 Antisense
AAGACTGTCGCAAAACCAACACCAG 467 153 433 Antisense
GCAAAACCAACACCAGAACTTCAGA 238 319 442 Antisense
TATGGCTGAAGAGAGCTCCACGCTT 18 655 543 Antisense
AGCTCCACGCTTCATTGTACGAGTT 497 79 556 Antisense
AAGCTGCAATTTGTCGAACTATGCG 503 149 657 Antisense
GAACTATGCGATTTCGGCAGATTTT 483 349 672 Antisense
GGGAAATATCGCTCACTTCTTCCCA 565 493 812 Antisense
CTTCTTCCCAAAACTATAAGCCATT 683 217 827 Antisense
Sequence Source Affymetrix Proprietary Database