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[176986_s_at]

Description Y55D5A.D /REP_DB=WormBase Gene ID /WP=CE22515 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAG23453.1
Cluster 6grp_total > 53      4grp_total > 04      6grp_cgc > 91      6grp_cgc_sub > 03      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 63
1 2 3 4 range
A 23.8
(-1.2)
A 		5.3
(-3)
A 		6.9
(-2.8)
A 		66.7
(3)
A 		61
B 10
(-1)
A 		8.3
(-1.5)
A 		3.4
(-3)
A 		23.1
(3)
A 		20
C 16.1
(-0.8)
A 		5.9
(-3)
A 		6.8
(-2.8)
A 		33.3
(3)
A 		27
avr 16.6
(-1.1) 		6.5
(-2.9) 		5.7
(-3) 		41
(3) 		35

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 45
1 2 3 4 5 6 range
A 22.3
(3)
A 		3.9
(-2.4)
A 		2.3
(-2.8)
A 		2.2
(-2.9)
A 		1.7
(-3)
A 		3.6
(-2.4)
A 		21
B 3.6
(-2.1)
A 		6.8
(-0.8)
A 		8.9
(0.1)
A 		16
(3)
A 		1.3
(-3)
A 		2.8
(-2.4)
A 		15
C 1.7
(-3)
A 		4.4
(-2.6)
A 		46.5
(3)
A 		2.3
(-2.9)
A 		13
(-1.5)
A 		10.7
(-1.8)
A 		45
avr 9.2
(-1.2) 		5
(-3) 		19.2
(3) 		6.8
(-2.2) 		5.3
(-2.9) 		5.7
(-2.7) 		14
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 9
1 2 3 4 range
A 8.7
(2.2)
A 		1.1
(-3)
A 		1.8
(-2.5)
A 		9.8
(3)
A 		9
B 3.2
(0.5)
A 		2.1
(-1.1)
A 		0.8
(-3)
A 		4.9
(3)
A 		4
C 5.3
(2.5)
A 		1.7
(-3)
A 		2.4
(-1.9)
A 		5.6
(3)
A 		4
avr 5.7
(1.7) 		1.6
(-3) 		1.7
(-2.9) 		6.8
(3) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 11
1 2 3 4 5 6 range
A 4.1
(3)
A 		1.4
(-1.5)
A 		0.5
(-3)
A 		0.6
(-2.8)
A 		0.5
(-3)
A 		0.9
(-2.3)
A 		4
B 1.3
(-1.7)
A 		2.2
(-0.2)
A 		2.2
(-0.2)
A 		4.2
(3)
A 		0.5
(-3)
A 		1
(-2.2)
A 		4
C 0.6
(-2.9)
A 		1.4
(-2.5)
A 		11.8
(3)
A 		0.5
(-3)
A 		4.2
(-1)
A 		3.6
(-1.4)
A 		11
avr 2
(-2.4) 		1.7
(-3) 		4.8
(3) 		1.8
(-2.8) 		1.7
(-3) 		1.8
(-2.8) 		3
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 176986_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18968
Sequence Type Exemplar sequence
Representative Public ID Y55D5A.D NCBI
Target Description Y55D5A.D /REP_DB=WormBase Gene ID /WP=CE22515 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAG23453.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065249(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065249 NCBI Caenorhabditis elegans abnormal DAuer Formation family member (daf-2) (daf-2) mRNA, complete cds. 11/11 A 
SNAP00000010554 ENSEMBL cdna:SNAP chromosome:CEL140:III:2993926:3052047:-1 11/11 None
GENEFINDER00000010558 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:2995909:3023699:-1 11/11 None
Y55D5A.5 ENSEMBL cdna:known chromosome:CEL140:III:2995753:3028790:-1 gene:Y55D5A.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:2995908-3001263(-) 98.31 100.0
Public Domain and Genome References
Gene Title
Gene Symbol daf-2
Ensembl Y55D5A.5
Entrez Gene 175410 Entrez gene
SwissProt Q968Y9 EMBL-EBI
WormBase CE27499 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.27874.1.S1_S_AT similar to Insulin receptor precursor (IR) (CD220 antigen) cfa
CANINE_2:CFAAFFX.27879.1.S1_S_AT similar to Insulin receptor precursor (IR) (CD220 antigen) cfa
DROSOPHILA_2:1629141_AT Insulin-like receptor dm
DROSGENOME1:143734_AT Insulin-like receptor dm
CHICKEN:GGA.3580.1.S1_AT insulin receptor gga
CHICKEN:GGA.3580.2.S1_A_AT insulin receptor gga
CHICKEN:GGAAFFX.2310.1.S1_AT insulin receptor gga
HG-U133_PLUS_2:207851_S_AT insulin receptor hs
HG-U133A:207851_S_AT insulin receptor hs
HG-U133A_2:207851_S_AT insulin receptor hs
HU35KSUBA:X02160_AT insulin receptor hs
HUGENEFL:X02160_AT insulin receptor hs
HG-U95AV2:1572_S_AT insulin receptor hs
HC-G110:1572_S_AT insulin receptor hs
HUGENEFL:M10051_S_AT insulin receptor hs
U133_X3P:G4557883_3P_A_AT insulin receptor hs
U133_X3P:HS.89695.2.A1_3P_AT insulin receptor hs
HG-U133A_2:213792_S_AT insulin receptor hs
HG-FOCUS:213792_S_AT insulin receptor hs
HG-U133_PLUS_2:213792_S_AT insulin receptor hs
HG-U133A:213792_S_AT insulin receptor hs
HG-U95AV2:33162_AT insulin receptor hs
HG-U133_PLUS_2:226212_S_AT Insulin receptor hs
HG-U133B:226212_S_AT Insulin receptor hs
HU35KSUBC:RC_T47312_AT Insulin receptor hs
HU35KSUBD:RC_T79740_AT Insulin receptor hs
HU35KSUBD:RC_AA437233_AT Insulin receptor hs
U133_X3P:HS.98401.0.S1_3P_AT Insulin receptor hs
U133_X3P:HS.208334.0.S2_3P_S_AT Insulin receptor hs
U133_X3P:HS.208334.0.S1_3P_AT Insulin receptor hs
HG-U133_PLUS_2:226216_AT Insulin receptor hs
HG-U133B:226216_AT Insulin receptor hs
HG-U133_PLUS_2:226450_AT Insulin receptor hs
HG-U133B:226450_AT Insulin receptor hs
HG-U95B:47628_AT Insulin receptor hs
HG-U95C:65110_AT Insulin receptor hs
HG-U133_PLUS_2:227432_S_AT Insulin receptor hs
HG-U133B:227432_S_AT Insulin receptor hs
HG-U133_PLUS_2:243002_AT Insulin receptor hs
HG-U133B:243002_AT Insulin receptor hs
HG-U95B:44163_AT Insulin receptor hs
HG-U95D:70578_AT Insulin receptor hs
HG-U95E:70604_AT Insulin receptor hs
HG-U95D:77743_AT Insulin receptor hs
HG-U95D:88319_AT Insulin receptor hs
HU35KSUBC:RC_AA342446_S_AT Insulin receptor hs
HU35KSUBB:RC_R05296_AT Insulin receptor hs
HU35KSUBB:RC_AA056206_AT Insulin receptor hs
U133_X3P:HS.270101.0.A1_3P_AT Insulin receptor hs
U133_X3P:HS.89695.1.A1_3P_AT Insulin receptor hs
RN-U34:M29014_AT insulin receptor rn
RG-U34A:M29014_AT insulin receptor rn
RAE230A:1369051_AT insulin receptor rn
RAT230_2:1369051_AT insulin receptor rn
RG-U34C:RC_AI070496_AT Insulin receptor rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6468 protein amino acid phosphorylation inferred from electronic annotation QuickGO AmiGO
15986 ATP synthesis coupled proton transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
16469 proton-transporting two-sector ATPase complex inferred from electronic annotation QuickGO AmiGO
5887 integral to plasma membrane inferred from sequence similarity QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4672 protein kinase activity inferred from electronic annotation QuickGO AmiGO
4674 protein serine/threonine kinase activity inferred from electronic annotation QuickGO AmiGO
4713 protein-tyrosine kinase activity inferred from electronic annotation QuickGO AmiGO
4714 transmembrane receptor protein tyrosine kinase activity inferred from electronic annotation QuickGO AmiGO
5006 epidermal growth factor receptor activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
46933 hydrogen-transporting ATP synthase activity, rotational mechanism inferred from electronic annotation QuickGO AmiGO
46961 hydrogen-transporting ATPase activity, rotational mechanism inferred from electronic annotation QuickGO AmiGO
5009 insulin receptor activity inferred from sequence similarity QuickGO AmiGO
5524 ATP binding inferred from sequence similarity QuickGO AmiGO
17046 peptide hormone binding inferred from sequence similarity QuickGO AmiGO
42169 SH2 domain binding inferred from sequence similarity QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK29947 Abnormal dauer formation protein 2 [Caenorhabditis elegans] ref|NP_497650.3| abnormal DAuer Formation family member (daf-2) [Caenorhabditis elegans] 0.0
blast AAC47715 insulin receptor homolog [Caenorhabditis elegans] 0.0
blast CAE69523 Hypothetical protein CBG15732 [Caenorhabditis briggsae] 0.0
blast AAQ90014 dauer abnormal formation-2 precursor [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR003961 EMBL-EBI Fibronectin, type III 1.7E-5
Pfam IPR003961 EMBL-EBI Fibronectin, type III 1.1E-4
Pfam IPR000719 EMBL-EBI Protein kinase 4.3E-67
Pfam IPR000494 EMBL-EBI Epidermal growth-factor receptor (EGFR), L domain 8.2E-36
Pfam IPR003961 EMBL-EBI Fibronectin, type III 1.7E-5
Pfam IPR003961 EMBL-EBI Fibronectin, type III 1.1E-4
Pfam IPR000719 EMBL-EBI Protein kinase 4.3E-67
Pfam IPR000494 EMBL-EBI Epidermal growth-factor receptor (EGFR), L domain 3.2E-27
Pfam IPR000494 EMBL-EBI Epidermal growth-factor receptor (EGFR), L domain 7.9E-12
Pfam IPR000494 EMBL-EBI Epidermal growth-factor receptor (EGFR), L domain 8.2E-36
Pfam IPR003961 EMBL-EBI Fibronectin, type III 1.7E-5
Pfam IPR003961 EMBL-EBI Fibronectin, type III 1.1E-4
Pfam IPR000719 EMBL-EBI Protein kinase 4.3E-67
Pfam IPR000494 EMBL-EBI Epidermal growth-factor receptor (EGFR), L domain 4.3E-44
Pfam IPR000494 EMBL-EBI Epidermal growth-factor receptor (EGFR), L domain 8.2E-36
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_497650.3 2 1182-1204,1307-1324
Y55D5A.5 2 1182-1204,1307-1324
SNAP00000010554 2 954-976,1079-1096
GENEFINDER00000010558 2 1296-1318,1421-1438
Sequence
Target Sequence 
>C. ELEGANS:176986_S_AT
gcggaagttcggacgtatgctggtgatggagattatgtggagagagatgttcgagagaat
gatgtgccaacgcgacgaaatactggtgcatcaacatcaagttacacaggtggtggtcca
tattgcctaacaaatcgtggtggttcaaatgaacgaggagccggtttcggtgaagcacgc
cggagcacgggcgcctcgagctcttcctacggtgttccacagacgaattggagtggaaat
cgtggtgccacgtattatacgagtaaagctcaacaggcagcaactgcagcagcagcagca
gcagcagctctccaacagcaacaaaatggtggtcgaggcgatcgattaactcaactaccc
ggaactggacatttacaatcgacacgtggtggacaagatggagattatattgaaactgaa
ccgaaaaattatagaaataatggatctccatcgcgaaacggcaacagccgtgacattttc
aacggacgttcggctttcggtgaaaatg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCGGAAGTTCGGACGTATGCTGGTG 652 289 1363 Antisense
GAGAATGATGTGCCAACGCGACGAA 360 391 1417 Antisense
AACGCGACGAAATACTGGTGCATCA 284 145 1431 Antisense
GTGGTGGTCCATATTGCCTAACAAA 327 489 1472 Antisense
ACGAGGAGCCGGTTTCGGTGAAGCA 127 93 1515 Antisense
TCTTCCTACGGTGTTCCACAGACGA 441 613 1564 Antisense
GGCGATCGATTAACTCAACTACCCG 345 539 1699 Antisense
TCAACTACCCGGAACTGGACATTTA 333 631 1713 Antisense
TAATGGATCTCCATCGCGAAACGGC 158 633 1800 Antisense
GGCAACAGCCGTGACATTTTCAACG 460 535 1822 Antisense
GGACGTTCGGCTTTCGGTGAAAATG 178 503 1846 Antisense
Sequence Source Affymetrix Proprietary Database