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[176985_at]

Description Y54G2A.A /REP_DB=WormBase Gene ID /WP=CE25443 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF59561.1
Cluster 6grp_highest_10_10 > 71      6grp_high1_10_10 > 28      4grp_highest_8_8 > 50      4grp_high_8_8 > 40      6grp_highest_nonorm > 50      6grp_total > 27      4grp_total > 46      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 599
1 2 3 4 range
A 792.5
(3)
P 		378.6
(-1.5)
P 		295.6
(-2.4)
P 		237.5
(-3)
P 		555
B 725.6
(3)
P 		455.4
(-2.2)
P 		413.9
(-3)
P 		506.6
(-1.2)
P 		312
C 735.7
(3)
P 		435.7
(-0.3)
P 		462.9
(-0)
P 		193.2
(-3)
P 		543
avr 751.3
(3) 		423.2
(-1.5) 		390.8
(-1.9) 		312.4
(-3) 		439

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1066
1 2 3 4 5 6 range
A 483.9
(0.4)
P 		662.6
(3)
P 		248.1
(-3)
P 		345.6
(-1.6)
P 		280.1
(-2.5)
P 		655
(2.9)
P 		415
B 593.2
(3)
P 		360.7
(-0.6)
P 		230.2
(-2.6)
P 		261.1
(-2.1)
P 		203.9
(-3)
P 		348.8
(-0.8)
P 		389
C 1270.2
(3)
P 		306.3
(-2.6)
P 		445.5
(-1.8)
P 		238.3
(-3)
P 		542.5
(-1.2)
P 		311.8
(-2.6)
P 		1032
avr 782.4
(3) 		443.2
(-1.1) 		307.9
(-2.7) 		281.7
(-3) 		342.2
(-2.3) 		438.5
(-1.1) 		501
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 256
1 2 3 4 range
A 288.5
(3)
P 		82.2
(-1.9)
P 		77.3
(-2)
P 		34.8
(-3)
P 		254
B 231.4
(3)
P 		112.5
(-2.3)
P 		95.9
(-3)
P 		108
(-2.5)
P 		136
C 243.5
(3)
P 		125.3
(-0.4)
P 		161.3
(0.7)
P 		32.6
(-3)
P 		211
avr 254.5
(3) 		106.7
(-1.5) 		111.5
(-1.4) 		58.5
(-3) 		196

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 423
1 2 3 4 5 6 range
A 89.2
(-1.8)
P 		235.7
(3)
P 		54
(-3)
P 		87.7
(-1.9)
P 		84.5
(-2)
P 		159.5
(0.5)
P 		182
B 206.4
(3)
P 		120.1
(-0.4)
P 		55.9
(-3)
P 		69.2
(-2.5)
P 		82.9
(-1.9)
P 		130.1
(-0)
P 		151
C 470.8
(3)
P 		93.5
(-2.4)
P 		113.4
(-2.1)
P 		47.9
(-3)
P 		175.6
(-1.2)
P 		104.4
(-2.2)
P 		423
avr 255.5
(3) 		149.8
(-0.4) 		74.4
(-2.8) 		68.3
(-3) 		114.3
(-1.5) 		131.3
(-1) 		187
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176985_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19208
Sequence Type Exemplar sequence
Representative Public ID Y54G2A.A NCBI
Target Description Y54G2A.A /REP_DB=WormBase Gene ID /WP=CE25443 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF59561.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067883(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067883 NCBI Caenorhabditis elegans Y67D8C.5 (Y67D8C.5) mRNA, complete cds. 11/11 A 
SNAP00000001608 ENSEMBL cdna:SNAP chromosome:CEL140:IV:3033190:3049968:-1 11/11 None
GENEFINDER00000001628 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:3033190:3049968:-1 11/11 None
Y67D8C.5 ENSEMBL cdna:known chromosome:CEL140:IV:3032726:3049968:-1 gene:Y67D8C.5 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:3033195-3036559(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y67D8C.5
Entrez Gene 177084 Entrez gene
SwissProt Q9GUP2 EMBL-EBI
WormBase CE31665 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6464 protein modification inferred from electronic annotation QuickGO AmiGO
6512 ubiquitin cycle inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5622 intracellular inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4842 ubiquitin-protein ligase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAG23375 Hypothetical protein Y67D8C.5 [Caenorhabditis elegans] ref|NP_500284.2| Y67D8C.5 [Caenorhabditis elegans] 0.0
blast CAE59883 Hypothetical protein CBG03363 [Caenorhabditis briggsae] 0.0
blast CAI42656 upstream regulatory element binding protein 1 (UREB1) [Homo sapiens] emb|CAI39581.1| upstream regulatory element binding protein 1 (UREB1) [Homo sapiens] 1.0E-172
Protein Domains
Database ID Description E-Value
Pfam IPR010309 EMBL-EBI Protein of unknown function DUF908 1.0E-126
Pfam IPR000449 EMBL-EBI Ubiquitin-associated domain 6.2E-9
Pfam IPR000569 EMBL-EBI HECT domain (Ubiquitin-protein ligase) 1.0E-126
Pfam IPR010314 EMBL-EBI Protein of unknown function DUF913 1.0E-126
Pfam IPR010309 EMBL-EBI Protein of unknown function DUF908 1.0E-126
Pfam IPR000449 EMBL-EBI Ubiquitin-associated domain 6.2E-9
Pfam IPR000569 EMBL-EBI HECT domain (Ubiquitin-protein ligase) 1.0E-126
Pfam IPR010314 EMBL-EBI Protein of unknown function DUF913 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:176985_AT
ctggctcttgatctgacattctccacagaagtcgaagagtttggagttcgatcggttcga
gacctgaaaccgaacggcaggaagattgaggtgaacgatgcgaacaaggacgagtatgtg
aagctcgtgtgccagatgaagatgaccggatcgattcggaagcagctcgacgcgttcctc
accggattctatgagatcattccgaaagatctgatatcgatgtttaatgagcaagagctt
gaactgctgatatctggacttccgactgttgatattgatgatatggcggcgaatacggat
tacaagggattccagaagacgtcgacgcatattcaatggttctggagagcacttcgatcg
tttgagaaggaggataaggcgaaattcctgcaatttgttaccggaacatcgaaggtgcca
ctgcaaggattcgcttcactggaaggcatgaacggagtacaaaagtttagtattcacatg
gatagccgtggtggagacagacttccagctgctcatacttgcttcaatcaactggattta
ccgcaatacgagtcatacgaaaagctacgtcaatcacttctcttg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTGGCTCTTGATCTGACATTCTCCA 455 241 2017 Antisense
GGAGTTCGATCGGTTCGAGACCTGA 674 515 2059 Antisense
GTGAAGCTCGTGTGCCAGATGAAGA 1 483 2134 Antisense
GATCGATTCGGAAGCAGCTCGACGC 326 417 2165 Antisense
AACTGCTGATATCTGGACTTCCGAC 704 141 2258 Antisense
GACGTCGACGCATATTCAATGGTTC 151 377 2334 Antisense
GTTCTGGAGAGCACTTCGATCGTTT 113 443 2355 Antisense
GAAGGTGCCACTGCAAGGATTCGCT 219 333 2427 Antisense
TGGAGACAGACTTCCAGCTGCTCAT 441 549 2508 Antisense
GCTGCTCATACTTGCTTCAATCAAC 588 297 2524 Antisense
AAAGCTACGTCAATCACTTCTCTTG 110 125 2577 Antisense
Sequence Source Affymetrix Proprietary Database