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[176983_at]

Description Y54G2A.D /REP_DB=WormBase Gene ID /WP=CE25446 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF59564.1
Cluster 6grp_total > 12      4grp_total > 02      6grp_total_sub > Hat      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 61
1 2 3 4 range
A 8.8
(-2.9)
A 		8.2
(-3)
A 		7.9
(-3)
A 		63.7
(3)
A 		56
B 11.1
(1.8)
A 		11
(1.5)
A 		9.5
(-3)
A 		11.5
(3)
A 		2
C 6
(-3)
A 		8.9
(-2.7)
A 		8.5
(-2.8)
A 		66.8
(3)
M 		61
avr 8.6
(-3) 		9.4
(-2.9) 		8.6
(-3) 		47.3
(3) 		39

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 24
1 2 3 4 5 6 range
A 8
(0.8)
A 		10
(2.8)
A 		4.1
(-3)
A 		10.2
(3)
A 		5.7
(-1.4)
A 		5.6
(-1.5)
A 		6
B 9.5
(-0.4)
A 		8.9
(-0.7)
A 		12.5
(1.2)
A 		15.9
(3)
A 		4.5
(-3)
A 		4.9
(-2.8)
A 		11
C 13.7
(-0.6)
A 		28.4
(3)
A 		15.8
(-0.1)
A 		9
(-1.8)
A 		8.5
(-1.9)
A 		4
(-3)
A 		24
avr 10.4
(0.1) 		15.8
(3) 		10.8
(0.3) 		11.7
(0.8) 		6.2
(-2.2) 		4.8
(-3) 		11
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 10
1 2 3 4 range
A 3.2
(-1.9)
A 		1.8
(-3)
A 		2.1
(-2.8)
A 		9.3
(3)
A 		8
B 3.5
(3)
A 		2.7
(-0.7)
A 		2.2
(-3)
A 		2.5
(-1.6)
A 		1
C 2
(-3)
A 		2.6
(-2.6)
A 		3
(-2.4)
A 		11.3
(3)
M 		9
avr 2.9
(-2.4) 		2.4
(-3) 		2.4
(-3) 		7.7
(3) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 8
1 2 3 4 5 6 range
A 1.5
(-1.6)
A 		3.5
(3)
A 		0.9
(-3)
A 		2.6
(0.9)
A 		1.7
(-1.2)
A 		1.4
(-1.8)
A 		3
B 3.3
(0.7)
A 		3
(-0)
A 		3
(-0)
A 		4.2
(3)
A 		1.8
(-3)
A 		1.8
(-3)
A 		2
C 5.1
(0)
A 		8.7
(3)
A 		4
(-0.9)
A 		1.8
(-2.7)
A 		2.7
(-1.9)
A 		1.4
(-3)
A 		7
avr 3.3
(0) 		5.1
(3) 		2.6
(-1.2) 		2.9
(-0.7) 		2.1
(-2) 		1.5
(-3) 		4
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176983_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19166
Sequence Type Exemplar sequence
Representative Public ID Y54G2A.D NCBI
Target Description Y54G2A.D /REP_DB=WormBase Gene ID /WP=CE25446 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF59564.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067877(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067877 NCBI Caenorhabditis elegans Y54G2A.27 (Y54G2A.27) mRNA, complete cds. 11/11 None
Y54G2A.27 ENSEMBL cdna:known chromosome:CEL140:IV:2998874:3000753:-1 gene:Y54G2A.27 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000036519 2/11 Cross Hyb Matching Probes None
GENEFINDER00000036613 2/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:2998879-3000759(-) 93.48 100.0
Public Domain and Genome References
Gene Title
Ensembl Y54G2A.27
Entrez Gene 190284 Entrez gene
SwissProt Q9N399 EMBL-EBI
WormBase CE25446 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAF59564 Hypothetical protein Y54G2A.27 [Caenorhabditis elegans] ref|NP_500278.1| Y54G2A.27 [Caenorhabditis elegans] 7.0E-62
Sequence
Target Sequence 
>C. ELEGANS:176983_AT
ggctggtacaaaacgtccgaagactcaattagaggagcttcttcagcaaccgctcaaaaa
aattgtctatcaagtgagaatttcgacaactcgtcgtcgtgtttcttctgccgtctttgt
ttatttacaaatgttgtttcgtccgtccgtgtgtcccctccatccatccatccatctgtc
atcatcatcatatatatatatatattcccaactacacatgctatcacacaacggcgggcc
gaccaccaccaccaccacaaccccaaaccgtccaaacggtacattcgaacaaaaacaatt
tatttccgccggcgtcaaccgtctgctcacctgcgcctcctcctcctcctcctcatcgcc
gccggtttcgattcctggtaaccgtagctttgccaacggggcggcggcagcggcttctca
acagcttgagggtccccccgacgtcttctactactactactgcttcttcttcttcttctt
tttcttctcaggacgtatcgatttgacccctcccggttggtcgccctcttctccccattc
cgttcattgccatcgtcgggttggttggtctt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGCTGGTACAAAACGTCCGAAGACT 354 539 78 Antisense
TAGAGGAGCTTCTTCAGCAACCGCT 520 653 107 Antisense
CGACAACTCGTCGTCGTGTTTCTTC 142 253 161 Antisense
TTCTTCTGCCGTCTTTGTTTATTTA 69 691 180 Antisense
ATTCCCAACTACACATGCTATCACA 146 9 281 Antisense
CTATCACACAACGGCGGGCCGACCA 657 223 298 Antisense
GATTCCTGGTAACCGTAGCTTTGCC 656 431 447 Antisense
GCTTCTCAACAGCTTGAGGGTCCCC 63 305 490 Antisense
TTCTTTTTCTTCTCAGGACGTATCG 176 689 553 Antisense
TCAGGACGTATCGATTTGACCCCTC 419 621 565 Antisense
TGCCATCGTCGGGTTGGTTGGTCTT 256 585 625 Antisense
Sequence Source Affymetrix Proprietary Database