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[176981_s_at] importin beta, nuclear transport factor

Description Y48G1A.C /REP_DB=WormBase Gene ID /WP=CE22166 /CHR=1 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF60681.1
Cluster 6grp_highest_10_10 > 96      6grp_high1_10_10 > 79      4grp_highest_8_8 > 09      4grp_high_8_8 > 61      6grp_highest_nonorm > 91      6grp_total > 79      4grp_total > 58      6grp_highest_sub > Cu      6grp_cgc > 49      6grp_high1_sub > Cu      6grp_cgc_sub > Da      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 160
1 2 3 4 range
A 233.9
(0.3)
P 		292.2
(3)
P 		282.8
(2.6)
P 		163.7
(-3)
P 		129
B 252.4
(-1)
P 		284.4
(0.8)
P 		324
(3)
P 		216.9
(-3)
P 		107
C 218.2
(-1)
P 		260.2
(2.8)
P 		262
(3)
P 		196.2
(-3)
P 		66
avr 234.8
(-0.4) 		278.9
(2.3) 		289.6
(3) 		192.3
(-3) 		97

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 261
1 2 3 4 5 6 range
A 217.8
(-2.2)
P 		207.7
(-2.4)
P 		189
(-2.9)
P 		186.6
(-3)
P 		324
(0.6)
P 		414.9
(3)
P 		228
B 189.5
(-2.5)
P 		231.5
(-1.2)
P 		234.6
(-1.1)
P 		173.8
(-3)
P 		197.5
(-2.3)
P 		364
(3)
P 		190
C 280
(2.1)
P 		210.4
(-0.7)
P 		197.8
(-1.2)
P 		153.6
(-3)
P 		299
(2.9)
P 		301.3
(3)
P 		148
avr 229.1
(-1.2) 		216.5
(-1.6) 		207.1
(-1.9) 		171.3
(-3) 		273.5
(0.2) 		360.1
(3) 		189
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 67
1 2 3 4 range
A 85.2
(3)
P 		63.4
(0.9)
P 		73.9
(1.9)
P 		24
(-3)
P 		61
B 80.5
(3)
P 		70.2
(1.2)
P 		75.1
(2.1)
P 		46.2
(-3)
P 		34
C 72.2
(1)
P 		74.8
(1.3)
P 		91.3
(3)
P 		33.1
(-3)
P 		58
avr 79.3
(2.9) 		69.5
(1.6) 		80.1
(3) 		34.4
(-3) 		46

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 105
1 2 3 4 5 6 range
A 40.1
(-3)
P 		73.9
(0.3)
P 		41.2
(-2.9)
P 		47.3
(-2.3)
P 		97.8
(2.7)
P 		101
(3)
P 		61
B 65.9
(-1.7)
P 		77
(-0.9)
P 		57
(-2.3)
P 		46
(-3)
P 		80.2
(-0.7)
P 		135.7
(3)
P 		90
C 103.8
(3)
P 		64.2
(-0.3)
P 		50.3
(-1.4)
P 		30.9
(-3)
P 		96.8
(2.4)
P 		100.9
(2.8)
P 		73
avr 69.9
(-0.6) 		71.7
(-0.4) 		49.5
(-2.3) 		41.4
(-3) 		91.6
(1.2) 		112.5
(3) 		71
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 176981_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18870
Sequence Type Exemplar sequence
Representative Public ID Y48G1A.C NCBI
Target Description Y48G1A.C /REP_DB=WormBase Gene ID /WP=CE22166 /CHR=1 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF60681.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058315(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058315 NCBI Caenorhabditis elegans IMportin Beta family member (imb-5) (imb-5) mRNA, complete cds. 11/11 None
SNAP00000006798 ENSEMBL cdna:SNAP chromosome:CEL140:I:316992:337675:-1 11/11 None
GENEFINDER00000006803 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:318826:323353:-1 11/11 None
Y48G1A.5 ENSEMBL cdna:known chromosome:CEL140:I:318552:323353:-1 gene:Y48G1A.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:318825-327053(-) 99.18 100.0
Public Domain and Genome References
Gene Title importin beta, nuclear transport factor
Gene Symbol imb-5
Ensembl Y48G1A.5
Entrez Gene 171621 Entrez gene
SwissProt Q965V4 EMBL-EBI
WormBase CE27001 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
59 protein import into nucleus, docking inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5634 nucleus inferred from electronic annotation QuickGO AmiGO
5643 nuclear pore inferred from electronic annotation QuickGO AmiGO
5737 cytoplasm inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
8565 protein transporter activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK84610 Importin beta family protein 5 [Caenorhabditis elegans] ref|NP_490716.1| IMportin Beta family member (imb-5) [Caenorhabditis elegans] 0.0
blast CAE74345 Hypothetical protein CBG22063 [Caenorhabditis briggsae] 0.0
blast NP_001307 CSE1 chromosome segregation 1-like protein isoform a [Homo sapiens] emb|CAI42818.1| CSE1 chromosome segregation 1-like (yeast) [Homo sapiens] emb|CAI19615.1| CSE1 chromosome segregation 1-like (yeast) [Homo sapiens] sp|P55060|CSE1_HUMAN Importin-alpha re-exporter (Chromosome segregation 1-like protein) (Cellular apoptosis susceptibility protein) gb|AAC35297.1| cellular apoptosis susceptibility protein [Homo sapiens] 1.0E-127
blast AAH73735 MGC84554 protein [Xenopus laevis] 1.0E-126
Protein Domains
Database ID Description E-Value
Pfam IPR005043 EMBL-EBI CAS/CSE, C-terminal 7.1E-9
Pfam IPR001494 EMBL-EBI Importin-beta, N-terminal 1.4E-14
Pfam IPR005043 EMBL-EBI CAS/CSE, C-terminal 7.1E-9
Pfam IPR001494 EMBL-EBI Importin-beta, N-terminal 1.4E-14
Sequence
Target Sequence 
>C. ELEGANS:176981_S_AT
gattcttccgtcgattgagcacttcgagggttccgcgatgacatttgtgctcaatacaat
gtttcgaagagttcaatcgtcgaagacacccaagtttatgaagctattcatcgtgttctt
gtgtcgatttacgatcgcccggagtgcacaggatcttgttcaatcgtgtgagaatattca
gactggcatgttcggaatgctcatcgagaaggtcgtatgcattgatttgccaggtctgaa
acaaacgacaacggggcctgagaaacggattattgccattggaatgggcaatttgctagc
cgatgtcacgcagcagcttgttggtcaatacggaattctgtcatacgaggtggcaatgct
gctggaagctgcttcagcctccgatcgagccgtcctatcaccagaagaagagcaagcttc
aatgtacaacgccgagggagaatttgtgaacccattctgtcgtctgagttacgctcccaa
gcaaccgcccgtcgccgccaacatcgccaaccacaaggcctactttgcgcaggc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATTCTTCCGTCGATTGAGCACTTC 341 429 2445 Antisense
GTTCCGCGATGACATTTGTGCTCAA 505 441 2474 Antisense
GAGTTCAATCGTCGAAGACACCCAA 259 399 2513 Antisense
CCCGGAGTGCACAGGATCTTGTTCA 269 259 2582 Antisense
GACTGGCATGTTCGGAATGCTCATC 13 377 2625 Antisense
TTGCTAGCCGATGTCACGCAGCAGC 241 703 2737 Antisense
GGAATTCTGTCATACGAGGTGGCAA 128 533 2776 Antisense
GGCAATGCTGCTGGAAGCTGCTTCA 314 535 2796 Antisense
GCAAGCTTCAATGTACAACGCCGAG 155 321 2856 Antisense
TGAACCCATTCTGTCGTCTGAGTTA 26 573 2891 Antisense
ACCACAAGGCCTACTTTGCGCAGGC 35 85 2954 Antisense
Sequence Source Affymetrix Proprietary Database