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[176970_at]

Description Y105E8B.G /REP_DB=WormBase Gene ID /WP=CE25179 /CHR=1 /FEA=Sanger Annotation /DEF=(HINXTON) protein_id:CAB60850.1
Cluster 6grp_total > 97      4grp_total > 32      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 63
1 2 3 4 range
A 73.3
(-2.8)
A 		72.5
(-3)
A 		98.5
(2.8)
P 		99.5
(3)
A 		27
B 75.6
(-3)
A 		95.8
(2.1)
A 		99.3
(3)
A 		75.6
(-3)
A 		24
C 44.4
(-3)
A 		79.1
(0.3)
A 		95
(1.8)
P 		107.5
(3)
A 		63
avr 64.4
(-3) 		82.5
(0.3) 		97.6
(3) 		94.2
(2.4) 		33

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 251
1 2 3 4 5 6 range
A 36.8
(-3)
A 		38.8
(-2.9)
A 		100.7
(-0.7)
M 		131
(0.4)
P 		156.6
(1.3)
P 		205.2
(3)
P 		168
B 66.1
(-2.9)
A 		108.2
(-1.8)
A 		63.3
(-3)
A 		84.3
(-2.4)
P 		175.7
(0)
P 		287.6
(3)
P 		224
C 46.4
(-3)
A 		100.9
(-1.2)
A 		103.5
(-1.1)
P 		90.3
(-1.5)
A 		125.4
(-0.3)
P 		223.6
(3)
P 		177
avr 49.8
(-3) 		82.6
(-2) 		89.2
(-1.7) 		101.9
(-1.3) 		152.6
(0.3) 		238.8
(3) 		189
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 19
1 2 3 4 range
A 26.7
(3)
A 		15.7
(-2.5)
A 		25.8
(2.6)
P 		14.6
(-3)
A 		12
B 24.1
(3)
A 		23.7
(2.7)
A 		23
(2.2)
A 		16.1
(-3)
A 		8
C 14.7
(-3)
A 		22.7
(-0.4)
A 		33.1
(3)
P 		18.1
(-1.9)
A 		18
avr 21.8
(0) 		20.7
(-0.6) 		27.3
(3) 		16.3
(-3) 		11

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 100
1 2 3 4 5 6 range
A 6.8
(-3)
A 		13.8
(-2)
A 		21.9
(-0.9)
M 		33.2
(0.7)
P 		47.2
(2.6)
P 		50
(3)
P 		43
B 23
(-2.5)
A 		36
(-1.7)
A 		15.4
(-3)
A 		22.3
(-2.5)
P 		71.4
(0.7)
P 		107.2
(3)
P 		92
C 17.2
(-3)
A 		30.8
(-1.6)
A 		26.3
(-2.1)
P 		18.2
(-2.9)
A 		40.6
(-0.6)
P 		74.9
(3)
P 		58
avr 15.7
(-3) 		26.9
(-1.9) 		21.2
(-2.5) 		24.6
(-2.1) 		53.1
(0.6) 		77.4
(3) 		62
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176970_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18798
Sequence Type Exemplar sequence
Representative Public ID Y105E8B.G NCBI
Target Description Y105E8B.G /REP_DB=WormBase Gene ID /WP=CE25179 /CHR=1 /FEA=Sanger Annotation /DEF=(HINXTON) protein_id:CAB60850.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_170940(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_170940 NCBI Caenorhabditis elegans Y105E8A.8 (Y105E8A.8) mRNA, complete cds. 11/11 A 
SNAP00000013328 ENSEMBL cdna:SNAP chromosome:CEL140:I:14409011:14420061:-1 11/11 None
GENEFINDER00000013491 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:14409011:14410686:-1 11/11 None
Y105E8A.8 ENSEMBL cdna:known chromosome:CEL140:I:14408875:14413767:-1 gene:Y105E8A.8 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:14408941-14409283(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y105E8A.8
Entrez Gene 173303 Entrez gene
SwissProt Q8WQB4 EMBL-EBI
WormBase CE29846 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAD21659 Hypothetical protein Y105E8A.8 [Caenorhabditis elegans] ref|NP_740936.1| Y105E8A.8 [Caenorhabditis elegans] 0.0
blast CAE64131 Hypothetical protein CBG08747 [Caenorhabditis briggsae] 1.0E-127
blast CAD21659 Hypothetical protein Y105E8A.8 [Caenorhabditis elegans] ref|NP_740936.1| Y105E8A.8 [Caenorhabditis elegans] 4.0E-98
blast CAE64131 Hypothetical protein CBG08747 [Caenorhabditis briggsae] 2.0E-59
blast NP_659073 hypothetical protein LOC216853 [Mus musculus] gb|AAH69868.1| Wdr79 protein [Mus musculus] gb|AAH21790.1| Hypothetical protein LOC216853 [Mus musculus] emb|CAI52013.1| novel protein [Mus musculus] 1.0E-14
Sequence
Target Sequence 
>C. ELEGANS:176970_AT
taaaacgtgacatgaccgccacacaccgtacacgattctccatcgacaaatccggacgtc
tcctattctcgggaacctctggaggtgacgtcatcgtctatgatcttcacgagtatagtg
aggagcttcagccggctcacgtgcaaaacgtggcatcccgatgtgttccctgtgtggatt
tgaaggggaagaagctggtgttgtgctccggggaacgagtgtatccggatgataagcttc
ttgga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TAAAACGTGACATGACCGCCACACA 280 639 35 Antisense
ACACGATTCTCCATCGACAAATCCG 193 111 64 Antisense
TCCTATTCTCGGGAACCTCTGGAGG 238 593 95 Antisense
GACGTCATCGTCTATGATCTTCACG 710 377 121 Antisense
TAGTGAGGAGCTTCAGCCGGCTCAC 244 653 150 Antisense
AGCCGGCTCACGTGCAAAACGTGGC 425 77 164 Antisense
AAAACGTGGCATCCCGATGTGTTCC 432 131 179 Antisense
GATGTGTTCCCTGTGTGGATTTGAA 81 409 194 Antisense
GAAGAAGCTGGTGTTGTGCTCCGGG 70 341 222 Antisense
GTGCTCCGGGGAACGAGTGTATCCG 359 477 237 Antisense
GTATCCGGATGATAAGCTTCTTGGA 145 455 255 Antisense
Sequence Source Affymetrix Proprietary Database