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[176966_at]

Description Y105E8B.N /REP_DB=WormBase Gene ID /WP=CE25709 /CHR=1 /FEA=Sanger Annotation /DEF=(HINXTON) protein_id:CAB60857.2
Cluster 6grp_highest_10_10 > 90      6grp_high1_10_10 > 19      4grp_highest_8_8 > 14      4grp_high_8_8 > 22      6grp_highest_nonorm > 81      6grp_total > 29      4grp_total > 17      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 584
1 2 3 4 range
A 275.4
(-3)
P
418.4
(-1.1)
P
331.3
(-2.3)
P
735
(3)
P
460
B 271.4
(-3)
P
342.2
(-2.2)
P
471.6
(-0.9)
P
833
(3)
P
562
C 248.9
(-3)
P
371.5
(-1.5)
P
373.3
(-1.5)
P
741.9
(3)
P
493
avr 265.2
(-3)
377.4
(-1.7)
392.1
(-1.5)
770
(3)
505

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 216
1 2 3 4 5 6 range
A 321.1
(0.7)
P
380
(2.4)
P
278.4
(-0.5)
P
189.7
(-3)
P
400.6
(3)
P
347.8
(1.5)
P
211
B 351.6
(3)
P
321.3
(1.6)
P
220.5
(-3)
P
285.8
(-0)
P
333.5
(2.2)
P
278.6
(-0.3)
P
131
C 387.5
(2.5)
P
247.3
(-1.9)
P
267.9
(-1.2)
P
233.3
(-2.3)
P
405.4
(3)
P
209.9
(-3)
P
196
avr 353.4
(1.9)
316.2
(0.3)
255.6
(-2.2)
236.3
(-3)
379.8
(3)
278.8
(-1.2)
144
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 95
1 2 3 4 range
A 100.3
(0.9)
P
90.8
(-1.8)
P
86.6
(-3)
P
107.6
(3)
P
21
B 86.6
(-2.9)
P
84.5
(-3)
P
109.3
(-1.4)
P
177.5
(3)
P
93
C 82.4
(-3)
P
106.8
(0.1)
P
130.1
(3)
P
125.1
(2.4)
P
48
avr 89.8
(-3)
94
(-2.5)
108.7
(-0.6)
136.7
(3)
47

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 97
1 2 3 4 5 6 range
A 59.2
(-2.2)
P
135.1
(3)
P
60.6
(-2.1)
P
48.1
(-3)
P
120.9
(2)
P
84.7
(-0.5)
P
87
B 122.3
(2)
P
106.9
(0.9)
P
53.5
(-3)
P
75.7
(-1.4)
P
135.5
(3)
P
103.9
(0.7)
P
82
C 143.6
(3)
P
75.5
(-1.2)
P
68.2
(-1.7)
P
46.9
(-3)
P
131.2
(2.2)
P
70.3
(-1.5)
P
97
avr 108.4
(1.3)
105.8
(1.1)
60.8
(-2.7)
56.9
(-3)
129.2
(3)
86.3
(-0.6)
72
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176966_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18786
Sequence Type Exemplar sequence
Representative Public ID Y105E8B.N NCBI
Target Description Y105E8B.N /REP_DB=WormBase Gene ID /WP=CE25709 /CHR=1 /FEA=Sanger Annotation /DEF=(HINXTON) protein_id:CAB60857.2
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_170944(11)
Transcript Assignments
Representative
Transcript
Description Matching Probes Related Probesets by Grade
NM_170944 NCBI Caenorhabditis elegans Y105E8A.12 (Y105E8A.12) mRNA, complete cds. 11/11 None
SNAP00000013331 ENSEMBL cdna:SNAP chromosome:CEL140:I:14443426:14466242:-1 11/11 None
GENEFINDER00000013501 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:14445159:14463961:-1 11/11 None
Y105E8A.12 ENSEMBL cdna:known chromosome:CEL140:I:14445026:14457136:-1 gene:Y105E8A.12 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:14445089-14449283(-) 97.26 100.0
Public Domain and Genome References
Gene Title
Ensembl Y105E8A.12
Entrez Gene 173307 Entrez gene
SwissProt Q8WQB1 EMBL-EBI
WormBase CE29832 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6812 cation transport inferred from electronic annotation QuickGO AmiGO
8152 metabolism inferred from electronic annotation QuickGO AmiGO
15992 proton transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3824 catalytic activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
15662 ATPase activity, coupled to transmembrane movement of ions, phosphorylative mechanism inferred from electronic annotation QuickGO AmiGO
16820 hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAD21662 Hypothetical protein Y105E8A.12 [Caenorhabditis elegans] ref|NP_740940.1| Y105E8A.12 [Caenorhabditis elegans] 0.0
blast CAE64135 Hypothetical protein CBG08751 [Caenorhabditis briggsae] 0.0
blast AAC24161 Hypothetical protein C02E7.1 [Caenorhabditis elegans] ref|NP_504328.1| C02E7.1 [Caenorhabditis elegans] 0.0
Protein Families
Method ID Description E-Value
ec A1A1_HUMAN (P05023) Sodium/potassium-transporting ATPase alpha-1 chain precursor (EC 3.6.3.9) (Sodium pump 1) (Na+/K+ ATPase 1) 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR008250 EMBL-EBI E1-E2 ATPase-associated region 9.3E-24
Pfam IPR006068 EMBL-EBI Cation transporting ATPase, C-terminal 4.2E-29
Pfam IPR005834 EMBL-EBI Haloacid dehalogenase-like hydrolase 2.3E-8
Pfam IPR005834 EMBL-EBI Haloacid dehalogenase-like hydrolase 1.8E-6
Pfam IPR006068 EMBL-EBI Cation transporting ATPase, C-terminal 1.0E-25
Pfam IPR008250 EMBL-EBI E1-E2 ATPase-associated region 5.3E-46
Pfam IPR005834 EMBL-EBI Haloacid dehalogenase-like hydrolase 5.1E-8
Pfam IPR005834 EMBL-EBI Haloacid dehalogenase-like hydrolase 1.8E-6
Pfam IPR004014 EMBL-EBI Cation transporting ATPase, N-terminal 5.6E-10
Pfam IPR006068 EMBL-EBI Cation transporting ATPase, C-terminal 4.2E-29
Pfam IPR008250 EMBL-EBI E1-E2 ATPase-associated region 3.3E-39
Pfam IPR005834 EMBL-EBI Haloacid dehalogenase-like hydrolase 5.1E-8
Pfam IPR005834 EMBL-EBI Haloacid dehalogenase-like hydrolase 1.8E-6
Pfam IPR004014 EMBL-EBI Cation transporting ATPase, N-terminal 5.6E-10
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_740940.1 5 94-116,123-145,640-662,738-760,775-792
Y105E8A.12 5 94-116,123-145,640-662,738-760,775-792
SNAP00000013331 8 132-154,158-180,324-346,356-378,944-966,1009-1031,1044-1066,1081-1098
GENEFINDER00000013501 7 147-166,173-195,359-381,388-410,983-1005,1081-1103,1118-1135
Sequence
Target Sequence
>C. ELEGANS:176966_AT
aaaacgacataatgcacactccgccaagatctcgaaccgcccgtcttctatccaaaagtc
ttcttgtctacgcgtacatcctcgcaggcttcggtatcactatcgggtgcattgcagcct
atcttagtgtttattggtaccataacatccctatcagtgacattctattcactgccgaac
accactggaagatcggagcaaagaacttcacgactagccaaggattgacgtttggtgagc
aacagcagttatttataaaggggcaggctgccgcggcatggcagatcacattagtcatgt
cacaagtcttccatttgtacaattgtacgaccaggagggtttccgttttccggcacggtc
tgactaatattgtcagcgtttttgcagttatcattgagatcctactcctgttcatgttcg
tgtacacaccggtcttccaatatat


BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position
Strandedness
AAAACGACATAATGCACACTCCGCC 602 131 665 Antisense
CAAAAGTCTTCTTGTCTACGCGTAC 312 191 717 Antisense
CAGGCTTCGGTATCACTATCGGGTG 505 205 749 Antisense
ATCGGGTGCATTGCAGCCTATCTTA 444 35 766 Antisense
GTACCATAACATCCCTATCAGTGAC 73 459 801 Antisense
TGACATTCTATTCACTGCCGAACAC 610 571 822 Antisense
GGCTGCCGCGGCATGGCAGATCACA 536 539 930 Antisense
AGTCATGTCACAAGTCTTCCATTTG 571 61 957 Antisense
GTTTTCCGGCACGGTCTGACTAATA 153 447 1009 Antisense
TACTCCTGTTCATGTTCGTGTACAC 205 647 1067 Antisense
TGTACACACCGGTCTTCCAATATAT 548 557 1085 Antisense
Sequence Source Affymetrix Proprietary Database