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[176962_s_at]

Description Y105E8B.F /REP_DB=WormBase Gene ID /WP=CE25708 /CHR=1 /FEA=Sanger Annotation /DEF=(HINXTON) protein_id:CAB60849.1
Cluster 6grp_highest_10_10 > 42      6grp_high1_10_10 > 15      4grp_highest_8_8 > 58      4grp_high_8_8 > 33      6grp_highest_nonorm > 60      6grp_total > 15      4grp_total > 46      6grp_highest_sub > 02      6grp_cgc > 33      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S2      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 765
1 2 3 4 range
A 761.8
(3)
P 		341.3
(-2)
P 		270.7
(-2.9)
P 		261.9
(-3)
P 		500
B 957.4
(3)
P 		423
(-1.2)
P 		192.6
(-3)
P 		217.9
(-2.8)
P 		765
C 497.6
(3)
P 		352.8
(-0.1)
P 		215.5
(-3)
P 		230.3
(-2.7)
P 		282
avr 738.9
(3) 		372.4
(-1.3) 		226.3
(-3) 		236.7
(-2.9) 		513

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 511
1 2 3 4 5 6 range
A 640.1
(2.8)
P 		654.8
(3)
P 		317.7
(-1)
P 		329.9
(-0.8)
P 		212.6
(-2.2)
P 		144.2
(-3)
P 		511
B 593.1
(3)
P 		520.8
(1.9)
P 		251.9
(-2.1)
P 		324
(-1)
P 		191.9
(-3)
P 		252.3
(-2.1)
P 		401
C 505.3
(1.6)
P 		603.8
(3)
P 		432.8
(0.6)
P 		197.9
(-2.7)
P 		269.8
(-1.7)
P 		179.4
(-3)
P 		424
avr 579.5
(2.8) 		593.1
(3) 		334.1
(-0.9) 		283.9
(-1.6) 		224.8
(-2.5) 		192
(-3) 		401
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 267
1 2 3 4 range
A 277.4
(3)
P 		74.1
(-2.1)
P 		70.8
(-2.2)
P 		38.3
(-3)
P 		239
B 305.3
(3)
P 		104.5
(-1.6)
P 		44.6
(-3)
P 		46.4
(-3)
P 		261
C 164.7
(3)
P 		101.4
(-0)
P 		75.1
(-1.3)
P 		38.8
(-3)
P 		126
avr 249.1
(3) 		93.3
(-1.5) 		63.5
(-2.4) 		41.2
(-3) 		208

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 198
1 2 3 4 5 6 range
A 118
(-0.5)
P 		232.9
(3)
P 		69.2
(-2)
P 		83.7
(-1.5)
P 		64.2
(-2.1)
P 		35.1
(-3)
P 		198
B 206.4
(3)
P 		173.4
(1.6)
P 		61.2
(-3)
P 		85.8
(-2)
P 		78
(-2.3)
P 		94.1
(-1.6)
P 		145
C 187.3
(3)
P 		184.3
(2.9)
P 		110.2
(-0.1)
P 		39.8
(-3)
P 		87.3
(-1.1)
P 		60.1
(-2.2)
P 		148
avr 170.6
(1.8) 		196.9
(3) 		80.2
(-2.2) 		69.8
(-2.7) 		76.5
(-2.4) 		63.1
(-3) 		134
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 176962_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18791
Sequence Type Exemplar sequence
Representative Public ID Y105E8B.F NCBI
Target Description Y105E8B.F /REP_DB=WormBase Gene ID /WP=CE25708 /CHR=1 /FEA=Sanger Annotation /DEF=(HINXTON) protein_id:CAB60849.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001026520(11), NM_001026521(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001026520 NCBI Caenorhabditis elegans EATing: abnormal pharyngeal pumping family member (eat-18) (eat-18) mRNA, complete cds. 11/11 None
NM_001026521 NCBI Caenorhabditis elegans EATing: abnormal pharyngeal pumping family member (eat-18) (eat-18) mRNA, complete cds. 11/11 None
GENEFINDER00000013504 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:14398501:14408023:1 11/11 None
SNAP00000013327 ENSEMBL cdna:SNAP chromosome:CEL140:I:14404471:14408284:1 11/11 None
Y105E8A.7a ENSEMBL cdna:known chromosome:CEL140:I:14398451:14408340:1 gene:Y105E8A.7 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
173302 4 2 NM_001026520 174766_at (11), 176962_s_at (11)
NM_001026521 174766_at (11), 176961_at (9), 176962_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:14400055-14407954(+) 99.94 100.0
Public Domain and Genome References
Ensembl Y105E8A.7
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAD21658 Hypothetical protein Y105E8A.7a [Caenorhabditis elegans] ref|NP_001021691.1| EATing: abnormal pharyngeal pumping family member (eat-18) [Caenorhabditis elegans] tpe|CAE82298.1| TPA: LEV-10A protein [Caenorhabditis elegans] 0.0
blast CAE64130 Hypothetical protein CBG08746 [Caenorhabditis briggsae] 0.0
blast CAF31497 Hypothetical protein Y105E8A.7b [Caenorhabditis elegans] ref|NP_001021692.1| EATing: abnormal pharyngeal pumping family member (eat-18) [Caenorhabditis elegans] tpe|CAE82299.1| TPA: LEV-10B [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000859 EMBL-EBI CUB 2.3E-10
Pfam IPR000859 EMBL-EBI CUB 3.9E-19
Pfam IPR000859 EMBL-EBI CUB 1.1E-6
Pfam IPR000859 EMBL-EBI CUB 9.3E-19
Pfam IPR000859 EMBL-EBI CUB 4.3E-13
Pfam IPR000859 EMBL-EBI CUB 2.3E-10
Pfam IPR000859 EMBL-EBI CUB 3.9E-19
Pfam IPR000859 EMBL-EBI CUB 1.1E-6
Pfam IPR000859 EMBL-EBI CUB 9.3E-19
Pfam IPR000859 EMBL-EBI CUB 4.3E-13
Pfam IPR000859 EMBL-EBI CUB 1.1E-6
Pfam IPR000859 EMBL-EBI CUB 9.3E-19
Pfam IPR000859 EMBL-EBI CUB 4.3E-13
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_001021691.1 1 810-832
NP_001021692.1 1 817-839
Y105E8A.7a 1 810-832
GENEFINDER00000013504 2 96-118,981-1003
SNAP00000013327 1 519-541
Sequence
Target Sequence 
>C. ELEGANS:176962_S_AT
gcgatcggacggtggtgattgcggcggtgttctgcggtataacatttgtgctcatttgca
tattcttcttgtatttgttcaagaaaaagctggagcggaagaagaagtcgaaacggaaaa
acgacacgaagcatcggcaacggcaaccataccggcagcagaggccaatgcacaagcagc
attgcgattcttca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCGATCGGACGGTGGTGATTGCGGC 117 291 2895 Antisense
CGGCGGTGTTCTGCGGTATAACATT 65 239 2916 Antisense
GTTCTGCGGTATAACATTTGTGCTC 396 443 2923 Antisense
TAACATTTGTGCTCATTTGCATATT 406 639 2934 Antisense
AAGCTGGAGCGGAAGAAGAAGTCGA 444 149 2981 Antisense
GAAACGGAAAAACGACACGAAGCAT 377 353 3004 Antisense
GACACGAAGCATCGGCAACGGCAAC 307 367 3017 Antisense
GGCAACCATACCGGCAGCAGAGGCC 531 533 3036 Antisense
TACCGGCAGCAGAGGCCAATGCACA 98 651 3044 Antisense
GCAGCAGAGGCCAATGCACAAGCAG 576 313 3049 Antisense
GCACAAGCAGCATTGCGATTCTTCA 600 317 3064 Antisense
Sequence Source Affymetrix Proprietary Database