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[176959_at]

Description Y105E8B.L /REP_DB=WormBase Gene ID /WP=CE25174 /CHR=1 /FEA=Sanger Annotation /DEF=(HINXTON) protein_id:CAB60855.1
Cluster 6grp_highest_10_10 > 32      6grp_high1_10_10 > 12      4grp_highest_8_8 > 41      4grp_high_8_8 > 49      6grp_highest_nonorm > 51      6grp_total > 23      4grp_total > 62      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 471
1 2 3 4 range
A 641.6
(3)
P 		489.7
(-0.4)
P 		558.7
(1.2)
P 		369.7
(-3)
P 		272
B 706.2
(3)
P 		634.3
(2)
P 		561.9
(0.9)
P 		287.2
(-3)
P 		419
C 758.5
(3)
P 		484.3
(-1.6)
P 		506.8
(-1.2)
P 		398.8
(-3)
P 		360
avr 702.1
(3) 		536.1
(0.2) 		542.5
(0.3) 		351.9
(-3) 		350

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 386
1 2 3 4 5 6 range
A 713
(3)
P 		611.7
(1.1)
P 		577.4
(0.5)
P 		437.6
(-2.2)
P 		393.9
(-3)
P 		422
(-2.5)
P 		319
B 689.8
(2.4)
P 		700.3
(2.6)
P 		726.9
(3)
P 		526.5
(-0.1)
P 		431.1
(-1.6)
P 		340.6
(-3)
P 		386
C 615.4
(2.1)
P 		664.6
(3)
P 		529.7
(0.5)
P 		484.1
(-0.4)
P 		451.4
(-1)
P 		343.7
(-3)
P 		321
avr 672.7
(3) 		658.9
(2.7) 		611.3
(1.8) 		482.7
(-0.8) 		425.5
(-1.9) 		368.8
(-3) 		304
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 197
1 2 3 4 range
A 233.6
(3)
P 		106.3
(-1.3)
P 		146.1
(0.1)
P 		54.1
(-3)
P 		180
B 225.2
(3)
P 		156.6
(0.5)
P 		130.2
(-0.5)
P 		61.2
(-3)
P 		164
C 251.1
(3)
P 		139.2
(-0.7)
P 		176.6
(0.6)
P 		67.2
(-3)
P 		184
avr 236.6
(3) 		134
(-0.5) 		151
(0.1) 		60.8
(-3) 		176

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 143
1 2 3 4 5 6 range
A 131.4
(-1.5)
P 		217.6
(3)
P 		125.7
(-1.8)
P 		111
(-2.6)
P 		118.9
(-2.2)
P 		102.8
(-3)
P 		115
B 240
(3)
P 		233.1
(2.6)
P 		176.5
(-0.4)
P 		139.4
(-2.3)
P 		175.1
(-0.4)
P 		127
(-3)
P 		113
C 228.1
(3)
P 		202.8
(1.8)
P 		134.8
(-1.3)
P 		97.3
(-3)
P 		146.1
(-0.8)
P 		115
(-2.2)
P 		131
avr 199.8
(2) 		217.8
(3) 		145.7
(-1.2) 		115.9
(-2.9) 		146.7
(-1.1) 		114.9
(-3) 		103
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176959_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18777
Sequence Type Exemplar sequence
Representative Public ID Y105E8B.L NCBI
Target Description Y105E8B.L /REP_DB=WormBase Gene ID /WP=CE25174 /CHR=1 /FEA=Sanger Annotation /DEF=(HINXTON) protein_id:CAB60855.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001026509(11), NM_001026510(11), NM_001026511(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001026509 NCBI Caenorhabditis elegans Y105E8A.10a (Y105E8A.10) mRNA, complete cds. 11/11 None
NM_001026510 NCBI Caenorhabditis elegans Y105E8A.10b (Y105E8A.10) mRNA, complete cds. 11/11 None
NM_001026511 NCBI Caenorhabditis elegans Y105E8A.10c (Y105E8A.10) mRNA, complete cds. 11/11 None
SNAP00000013329 ENSEMBL cdna:SNAP chromosome:CEL140:I:14409618:14441788:1 11/11 None
GENEFINDER00000013515 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:14430534:14439801:1 11/11 None
Y105E8A.10a ENSEMBL cdna:known chromosome:CEL140:I:14426315:14439922:1 gene:Y105E8A.10 11/11 None
Y105E8A.10c.2 ENSEMBL cdna:known chromosome:CEL140:I:14426359:14439801:1 gene:Y105E8A.10 11/11 None
Y105E8A.10b ENSEMBL cdna:known chromosome:CEL140:I:14430298:14439801:1 gene:Y105E8A.10 11/11 None
Y105E8A.10c.1 ENSEMBL cdna:known chromosome:CEL140:I:14430531:14439801:1 gene:Y105E8A.10 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:14430464-14439732(+) 100.0 100.0
Public Domain and Genome References
Ensembl Y105E8A.10
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAD21661 Hypothetical protein Y105E8A.10a [Caenorhabditis elegans] ref|NP_001021680.1| Y105E8A.10a [Caenorhabditis elegans] 0.0
blast CAC48128 Hypothetical protein Y105E8A.10c [Caenorhabditis elegans] ref|NP_001021682.1| Y105E8A.10c [Caenorhabditis elegans] 0.0
blast CAH04764 Hypothetical protein Y105E8A.10b [Caenorhabditis elegans] ref|NP_001021681.1| Y105E8A.10b [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR006775 EMBL-EBI Protein of unknown function DUF608 1.0E-126
Pfam IPR006775 EMBL-EBI Protein of unknown function DUF608 1.0E-126
Pfam IPR006775 EMBL-EBI Protein of unknown function DUF608 1.0E-126
Pfam IPR002634 EMBL-EBI BolA-like protein 2.7E-13
Pfam IPR002553 EMBL-EBI Adaptin, N-terminal 1.0E-126
Pfam IPR008152 EMBL-EBI Alpha/gamma adaptin, C-terminal 1.4E-13
Pfam IPR006775 EMBL-EBI Protein of unknown function DUF608 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:176959_AT
ttggttccttcaatcagtatctcctgaactagccgacgagcttctcccgaatcatatggt
ccgatcagcgttggatacgatttatcgattgaatgtgtgtaaatttggaaacggacgaat
gggtgcagtaaatggaatgaaaccgtcaggagtcgtagatcgggagtacattcaggctga
tgaaatgtggacgggtgttacttatgcagttgcatcgttacttattcaacagggagaagt
ggaaaaggcgtttcatacggcgtcggggagttatctgacatgttttgaggagactggact
acaatatcagacgcctgaagcactttatgaatcgaaattctaccgagccattggttacat
gcgtcctctatcgatttgggcaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTGGTTCCTTCAATCAGTATCTCCT 707 705 2181 Antisense
GTATCTCCTGAACTAGCCGACGAGC 295 455 2197 Antisense
CTTCTCCCGAATCATATGGTCCGAT 687 217 2221 Antisense
ATGGTCCGATCAGCGTTGGATACGA 565 47 2236 Antisense
GTGGACGGGTGTTACTTATGCAGTT 404 489 2367 Antisense
ATGCAGTTGCATCGTTACTTATTCA 527 43 2384 Antisense
GAAAAGGCGTTTCATACGGCGTCGG 358 285 2422 Antisense
ACGGCGTCGGGGAGTTATCTGACAT 390 95 2437 Antisense
ATCAGACGCCTGAAGCACTTTATGA 560 29 2486 Antisense
GAGCCATTGGTTACATGCGTCCTCT 214 385 2525 Antisense
ATGCGTCCTCTATCGATTTGGGCAA 34 41 2539 Antisense
Sequence Source Affymetrix Proprietary Database