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[176955_at]

Description Y105E8B.B /REP_DB=WormBase Gene ID /WP=CE25707 /CHR=1 /FEA=Sanger Annotation /DEF=(HINXTON) protein_id:CAB60845.1
Cluster 6grp_highest_10_10 > 26      6grp_high1_10_10 > 52      4grp_highest_8_8 > 19      4grp_high_8_8 > 47      6grp_highest_nonorm > 34      6grp_total > 52      4grp_total > 40      6grp_highest_sub > 03      6grp_cgc > 92      6grp_high1_sub > 03      6grp_cgc_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1287
1 2 3 4 range
A 1061
(-3)
P 		1563
(-0.7)
P 		2348.1
(3)
P 		1477.8
(-1.1)
P 		1287
B 1161.1
(-3)
P 		1367.9
(-1.5)
P 		1976.9
(3)
P 		1377.3
(-1.4)
P 		816
C 1096.6
(-3)
P 		1647.2
(1.6)
P 		1807.9
(3)
P 		1337.8
(-1)
P 		711
avr 1106.2
(-3) 		1526
(-0.3) 		2044.3
(3) 		1397.6
(-1.1) 		938

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 4987
1 2 3 4 5 6 range
A 1432
(-2.8)
P 		2218.3
(-1.8)
P 		6108.5
(3)
P 		3170
(-0.6)
P 		1725
(-2.4)
P 		1241.4
(-3)
P 		4867
B 1222.4
(-3)
P 		2537.5
(-1)
P 		5099.9
(3)
P 		2971.3
(-0.3)
P 		2167.2
(-1.5)
P 		1299.6
(-2.9)
P 		3878
C 1382.5
(-2.7)
P 		2272.2
(-1.5)
P 		5835.2
(3)
P 		2924
(-0.7)
P 		2354.5
(-1.4)
P 		1121.5
(-3)
P 		4714
avr 1345.6
(-2.8) 		2342.7
(-1.5) 		5681.2
(3) 		3021.8
(-0.6) 		2082.2
(-1.8) 		1220.8
(-3) 		4460
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 414
1 2 3 4 range
A 386.3
(-0.4)
P 		339.3
(-1.1)
P 		613.8
(3)
P 		216.3
(-3)
P 		398
B 370.3
(-0.2)
P 		337.8
(-1.4)
P 		458.1
(3)
P 		293.5
(-3)
P 		165
C 363
(-1)
P 		473.6
(0.7)
P 		630.1
(3)
P 		225.6
(-3)
P 		405
avr 373.2
(-0.6) 		383.6
(-0.4) 		567.3
(3) 		245.1
(-3) 		322

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1221
1 2 3 4 5 6 range
A 264
(-3)
P 		789
(-0)
P 		1330.3
(3)
P 		804
(0)
P 		520.5
(-1.6)
P 		302.2
(-2.8)
P 		1066
B 425.3
(-3)
P 		844.6
(0.1)
P 		1238.4
(3)
P 		787
(-0.3)
P 		880.5
(0.4)
P 		484.6
(-2.6)
P 		813
C 512.5
(-2.3)
P 		693.3
(-1.3)
P 		1485.2
(3)
P 		588
(-1.9)
P 		761.9
(-0.9)
P 		375.4
(-3)
P 		1110
avr 400.6
(-2.9) 		775.6
(-0.6) 		1351.3
(3) 		726.3
(-0.9) 		721
(-0.9) 		387.4
(-3) 		964
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176955_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18773
Sequence Type Exemplar sequence
Representative Public ID Y105E8B.B NCBI
Target Description Y105E8B.B /REP_DB=WormBase Gene ID /WP=CE25707 /CHR=1 /FEA=Sanger Annotation /DEF=(HINXTON) protein_id:CAB60845.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_170936(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_170936 NCBI Caenorhabditis elegans Enoyl-CoA Hydratase family member (ech-7) (ech-7) mRNA, complete cds. 11/11 None
SNAP00000013321 ENSEMBL cdna:SNAP chromosome:CEL140:I:14355855:14384135:-1 11/11 None
GENEFINDER00000013503 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:14375736:14377890:-1 11/11 None
Y105E8A.4 ENSEMBL cdna:known chromosome:CEL140:I:14375725:14377890:-1 gene:Y105E8A.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:14375666-14377821(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol ech-7
Ensembl Y105E8A.4
Entrez Gene 173300 Entrez gene
SwissProt Q9NEZ8 EMBL-EBI
WormBase CE25707 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
8152 metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3824 catalytic activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAC48119 Hypothetical protein Y105E8A.5 [Caenorhabditis elegans] ref|NP_740933.1| BBS (Bardet-Biedl Syndrome) protein family member (bbs-1) [Caenorhabditis elegans] 0.0
blast CAB60843 Hypothetical protein Y105E8A.2 [Caenorhabditis elegans] ref|NP_740930.2| Y105E8A.2 [Caenorhabditis elegans] 0.0
blast CAE64125 Hypothetical protein CBG08741 [Caenorhabditis briggsae] 0.0
blast CAC48118 Hypothetical protein Y105E8A.4 [Caenorhabditis elegans] ref|NP_740932.1| Enoyl-CoA Hydratase family member (ech-7) [Caenorhabditis elegans] 1.0E-132
blast CAE64127 Hypothetical protein CBG08743 [Caenorhabditis briggsae] 1.0E-111
blast CAA81594 Hypothetical protein T05G5.6 [Caenorhabditis elegans] ref|NP_499156.1| Enoyl-CoA Hydratase family member (ech-6) [Caenorhabditis elegans] sp|P34559|ECHM_CAEEL Probable enoyl-CoA hydratase, mitochondrial 5.0E-80
Protein Domains
Database ID Description E-Value
Pfam IPR001753 EMBL-EBI Enoyl-CoA hydratase/isomerase 4.7E-79
Pfam IPR001753 EMBL-EBI Enoyl-CoA hydratase/isomerase 4.7E-79
Sequence
Target Sequence 
>C. ELEGANS:176955_AT
attcgctgccggagccgatatcaaggagatggccaagttggagtttgcagatgtttttga
gaatgactatttcacgaactgggacaccttatcacacatcacaaaacctgtgatcgccgc
tgtcaacggatttgccttaggcggaggcacggaacttgccttaatgtgtgatatagtata
tgctggagagaatgcaatattcggccaaccggaaatcaccatcggaaccatccccggatt
aggtggtacccaacgatggccccggtatgtgagcaagtcagttgccatggaaatatgcct
atcaggcgaccgactaggcgctcaggaggcgaaggaagacggcctggtgagcaaggtgtt
cccggtgcagcagttggttggggaggctgttctgttggctgataggatcgctaagaattc
tcctcttattgtgaaaactgtcaagagatccctaaactcggcctaccaaaccagcctaaa
tcagggccttgaaatggaaaagcagttgttccagtcgacctt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATTCGCTGCCGGAGCCGATATCAAG 185 7 189 Antisense
CGAACTGGGACACCTTATCACACAT 89 253 263 Antisense
TGATCGCCGCTGTCAACGGATTTGC 549 565 299 Antisense
TCGGAACCATCCCCGGATTAGGTGG 169 601 410 Antisense
GATGGCCCCGGTATGTGAGCAAGTC 261 403 443 Antisense
TATGCCTATCAGGCGACCGACTAGG 570 657 482 Antisense
GCCTGGTGAGCAAGGTGTTCCCGGT 330 281 530 Antisense
GTTCCCGGTGCAGCAGTTGGTTGGG 586 441 546 Antisense
GGCTGTTCTGTTGGCTGATAGGATC 156 541 573 Antisense
TCAAGAGATCCCTAAACTCGGCCTA 569 631 629 Antisense
AAAAGCAGTTGTTCCAGTCGACCTT 381 131 686 Antisense
Sequence Source Affymetrix Proprietary Database