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[176954_at]

Description Y48G9A.M /REP_DB=WormBase Gene ID /WP=CE25330 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF60719.1
Cluster 4grp_high_8_8 > 07      6grp_total > 54      4grp_total > 07      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 67
1 2 3 4 range
A 89.3
(-0.6)
P 		65.4
(-3)
P 		67.3
(-2.8)
M 		125.5
(3)
P 		60
B 62.2
(-3)
P 		65.6
(-2.3)
P 		93.4
(3)
M 		89.1
(2.2)
P 		31
C 128.7
(3)
P 		103
(0.2)
P 		105.4
(0.4)
P 		74.3
(-3)
M 		54
avr 93.4
(2) 		78
(-3) 		88.7
(0.5) 		96.3
(3) 		18

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 68
1 2 3 4 5 6 range
A 49.1
(-0.3)
A 		85.5
(3)
P 		19.9
(-3)
A 		51.9
(-0.1)
A 		45.8
(-0.6)
A 		84.5
(2.9)
A 		66
B 21.9
(-3)
A 		35.8
(-1.3)
A 		71.4
(3)
A 		48.3
(0.2)
A 		35.8
(-1.3)
A 		36.6
(-1.2)
A 		50
C 44.5
(-0.4)
A 		38.6
(-1)
P 		75.6
(2.6)
A 		17.8
(-3)
A 		79.8
(3)
A 		37.1
(-1.1)
A 		62
avr 38.5
(-3) 		53.3
(2.2) 		55.6
(3) 		39.3
(-2.7) 		53.8
(2.4) 		52.7
(2) 		17
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 30
1 2 3 4 range
A 32.5
(3)
P 		14.2
(-3)
P 		17.6
(-1.9)
M 		18.4
(-1.6)
P 		18
B 19.8
(1)
P 		16.2
(-3)
P 		21.6
(3)
M 		19
(0.1)
P 		5
C 42.6
(3)
P 		29.6
(0.4)
P 		36.7
(1.8)
P 		12.5
(-3)
M 		30
avr 31.6
(3) 		20
(-1.6) 		25.3
(0.5) 		16.6
(-3) 		15

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 27
1 2 3 4 5 6 range
A 9
(-1.9)
A 		30.4
(3)
P 		4.3
(-3)
A 		13.2
(-1)
A 		13.8
(-0.8)
A 		20.6
(0.7)
A 		26
B 7.6
(-3)
A 		11.9
(-0.3)
A 		17.3
(3)
A 		12.8
(0.2)
A 		14.5
(1.3)
A 		13.6
(0.7)
A 		10
C 16.5
(0.5)
A 		11.8
(-0.8)
P 		19.2
(1.2)
A 		3.6
(-3)
A 		25.8
(3)
A 		12.4
(-0.6)
A 		22
avr 11
(-2.2) 		18
(3) 		13.6
(-0.3) 		9.9
(-3) 		18
(3) 		15.5
(1.1) 		8
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176954_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19009
Sequence Type Exemplar sequence
Representative Public ID Y48G9A.M NCBI
Target Description Y48G9A.M /REP_DB=WormBase Gene ID /WP=CE25330 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF60719.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065105(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065105 NCBI Caenorhabditis elegans Y48G9A.3 (Y48G9A.3) mRNA, complete cds. 11/11 None
SNAP00000015834 ENSEMBL cdna:SNAP chromosome:CEL140:III:2130533:2170683:1 11/11 None
Y48G9A.3 ENSEMBL cdna:known chromosome:CEL140:III:2151956:2157824:1 gene:Y48G9A.3 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000015839 6/11 Cross Hyb Matching Probes None
GENEFINDER00000015836 4/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:2154344-2157824(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y48G9A.3
Entrez Gene 190051 Entrez gene
SwissProt Q9N3M5 EMBL-EBI
WormBase CE25330 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK29918 Hypothetical protein Y48G9A.3 [Caenorhabditis elegans] ref|NP_497506.1| Y48G9A.3 [Caenorhabditis elegans] 0.0
blast AAK29921 Hypothetical protein Y48G9A.4 [Caenorhabditis elegans] ref|NP_497505.3| Y48G9A.4 [Caenorhabditis elegans] 0.0
blast CAE69457 Hypothetical protein CBG15650 [Caenorhabditis briggsae] 0.0
blast CAE69455 Hypothetical protein CBG15647 [Caenorhabditis briggsae] 1.0E-138
blast NP_006827 GCN1 general control of amino-acid synthesis 1-like 1 [Homo sapiens] 8.0E-13
Protein Domains
Database ID Description E-Value
scop a.2.7.Phenylalanyl-tRNA synthetase (PheRS) All alpha proteins; Long alpha-hairpin; tRNA-binding arm; Phenylalanyl-tRNA synthetase (PheRS) 4.80000019073486
scop a.4.5.Plant O-methyltransferase, N-terminal domain All alpha proteins; DNA/RNA-binding 3-helical bundle; "Winged helix" DNA-binding domain; Plant O-methyltransferase, N-terminal domain 4.19999980926514
Pfam IPR010472 EMBL-EBI Diaphanous FH3 2.0E-75
Pfam IPR010473 EMBL-EBI Diaphanous GTPase-binding 2.9E-86
Pfam IPR003104 EMBL-EBI Actin-binding FH2 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:176954_AT
aaatctacgatttcctcgctttgtaacacggatccggagattcgtgaagctggaatcggg
attgtggtggctttaagccgaaaatcggagatttcagtgattcagaagattctcaacacg
cttctcgatcaattttcggctgcaaaagcctcggaaattaagtgctcactgatggaaggt
gttggagcaattgcaaaggttgtggaagctcaggatattgataaaatcgcagaagatgtg
atttcaaagacggcaaaggcggataaagaatctcacgacggaacaattgaagctcaatgg
aatgctgctgcagaatgggcaattcgactgtcaaaaccggcaatttcactgacaaatgcg
ttcaaagaggcttcgaaattggcgccagccgtcaaatatatcggatatcgagttttggcg
gacattttggaacgaagatcgatgacgtcacttcccgaaggaatcgacgtgaagccactg
ctcaccgagctcgaaaccggaccaaaaggcgaactttgctccattggattggctcttttg
attcttcgaac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAATCTACGATTTCCTCGCTTTGTA 243 117 535 Antisense
CCTCGCTTTGTAACACGGATCCGGA 543 261 548 Antisense
GGAATCGGGATTGTGGTGGCTTTAA 157 531 586 Antisense
TTCTCAACACGCTTCTCGATCAATT 691 691 644 Antisense
GACTGTCAAAACCGGCAATTTCACT 213 375 860 Antisense
GAGGCTTCGAAATTGGCGCCAGCCG 537 405 901 Antisense
GATGACGTCACTTCCCGAAGGAATC 371 411 975 Antisense
GAATCGACGTGAAGCCACTGCTCAC 354 329 995 Antisense
TCACCGAGCTCGAAACCGGACCAAA 439 621 1016 Antisense
GGCGAACTTTGCTCCATTGGATTGG 297 541 1042 Antisense
GATTGGCTCTTTTGATTCTTCGAAC 248 435 1061 Antisense
Sequence Source Affymetrix Proprietary Database