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[176949_at]

Description Y48G9A.L /REP_DB=WormBase Gene ID /WP=CE25329 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF60718.1
Cluster 6grp_total > 25      4grp_total > 44      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 78
1 2 3 4 range
A 68.7
(-3)
A 		81.2
(-0.9)
A 		78.9
(-1.3)
A 		104.3
(3)
A 		36
B 92.4
(3)
A 		77.3
(0.3)
A 		79.6
(0.7)
A 		58.7
(-3)
A 		34
C 97
(0.2)
A 		133.3
(3)
A 		82.7
(-0.9)
A 		55.6
(-3)
A 		78
avr 86
(0.2) 		97.3
(3) 		80.4
(-1.2) 		72.9
(-3) 		24

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 85
1 2 3 4 5 6 range
A 27.3
(-1.1)
A 		61.8
(2.4)
A 		67.4
(3)
A 		8.4
(-3)
A 		11.9
(-2.6)
A 		38.7
(0.1)
A 		59
B 56.6
(0.8)
A 		72.4
(3)
A 		50.4
(-0.1)
A 		48.1
(-0.4)
A 		54.3
(0.5)
A 		29.2
(-3)
A 		43
C 93.4
(3)
A 		57.4
(-0.8)
A 		38.1
(-2.8)
A 		36.4
(-3)
A 		52
(-1.4)
A 		41.2
(-2.5)
A 		57
avr 59.1
(2.1) 		63.9
(3) 		52
(0.8) 		31
(-3) 		39.4
(-1.5) 		36.4
(-2) 		33
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 29
1 2 3 4 range
A 25
(3)
A 		17.6
(-1.6)
A 		20.6
(0.3)
A 		15.3
(-3)
A 		10
B 29.5
(3)
A 		19.1
(-0.7)
A 		18.4
(-0.9)
A 		12.5
(-3)
A 		17
C 32.1
(1.7)
A 		38.3
(3)
A 		28.8
(1)
A 		9.4
(-3)
A 		29
avr 28.9
(3) 		25
(1.6) 		22.6
(0.7) 		12.4
(-3) 		17

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 33
1 2 3 4 5 6 range
A 5
(-2.1)
A 		22
(3)
A 		14.7
(0.8)
A 		2.1
(-3)
A 		3.6
(-2.5)
A 		9.4
(-0.8)
A 		20
B 19.7
(1)
A 		24.1
(3)
A 		12.2
(-2.4)
A 		12.7
(-2.2)
A 		22.1
(2.1)
A 		10.9
(-3)
A 		13
C 34.6
(3)
A 		17.5
(-0.8)
A 		9.7
(-2.5)
A 		7.3
(-3)
A 		16.8
(-0.9)
A 		13.8
(-1.6)
A 		27
avr 19.8
(2.4) 		21.2
(3) 		12.2
(-0.9) 		7.4
(-3) 		14.2
(-0) 		11.4
(-1.3) 		14
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176949_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19063
Sequence Type Exemplar sequence
Representative Public ID Y48G9A.L NCBI
Target Description Y48G9A.L /REP_DB=WormBase Gene ID /WP=CE25329 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF60718.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065105, NM_065104
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
SNAP00000015834 ENSEMBL cdna:SNAP chromosome:CEL140:III:2130533:2170683:1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_065104 7/11 Cross Hyb Matching Probes None
NM_065105 4/11 Cross Hyb Matching Probes None
GENEFINDER00000015837 7/11 Cross Hyb Matching Probes None
GENEFINDER00000015848 4/11 Cross Hyb Matching Probes None
Y48G9A.4 7/11 Cross Hyb Matching Probes None
Y48G9A.3 4/11 Cross Hyb Matching Probes A 
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:2136405-2152478(+) 97.22 100.0
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK29921 Hypothetical protein Y48G9A.4 [Caenorhabditis elegans] ref|NP_497505.3| Y48G9A.4 [Caenorhabditis elegans] 0.0
blast AAK29918 Hypothetical protein Y48G9A.3 [Caenorhabditis elegans] ref|NP_497506.1| Y48G9A.3 [Caenorhabditis elegans] 0.0
blast CAE69457 Hypothetical protein CBG15650 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
scop a.2.7.Phenylalanyl-tRNA synthetase (PheRS) All alpha proteins; Long alpha-hairpin; tRNA-binding arm; Phenylalanyl-tRNA synthetase (PheRS) 4.80000019073486
scop a.4.5.Plant O-methyltransferase, N-terminal domain All alpha proteins; DNA/RNA-binding 3-helical bundle; "Winged helix" DNA-binding domain; Plant O-methyltransferase, N-terminal domain 4.19999980926514
Pfam IPR010472 EMBL-EBI Diaphanous FH3 2.0E-75
Pfam IPR010473 EMBL-EBI Diaphanous GTPase-binding 2.9E-86
Pfam IPR003104 EMBL-EBI Actin-binding FH2 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:176949_AT
gagaagcgtcgccaggaggaagctgaacgtcgagctcgcgtggcgagcacaaaatcatcg
gcttcatctgaacaggaccaattaatgatggaattggcggaaaaagtcggcggaatggga
attggcaagcggccgcgtggaaaaatcgatagtacacgaatggatcacggagatttcgag
aagctgatgaatggtctcaaacacacgggcggcaccactccgaatcgccgaaaaatgtca
aaatcgccgtctccagcgccacgtggctccgcaccaccgccagttgctccaaaaactcga
gtcgaggacgcattaaaggaagaaatccgccgtttttcgtcggcagtttcgaatccaagc
cttcgggctcatggaatggcctacgccgctctgacgaagctcatcgagcgccgctcaacg
gaaattccggctcaatttgtgaaggggctcgtcaaaatgagcattgccaactcgattcgg
ctct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGAAGCGTCGCCAGGAGGAAGCTG 111 393 2950 Antisense
AGGAAGCTGAACGTCGAGCTCGCGT 124 55 2966 Antisense
GGCGAGCACAAAATCATCGGCTTCA 549 539 2991 Antisense
GAATTGGCAAGCGGCCGCGTGGAAA 411 321 3068 Antisense
TGAATGGTCTCAAACACACGGGCGG 598 571 3137 Antisense
CACCACTCCGAATCGCCGAAAAATG 594 199 3162 Antisense
AAAATGTCAAAATCGCCGTCTCCAG 13 133 3181 Antisense
TTCGAATCCAAGCCTTCGGGCTCAT 346 691 3297 Antisense
CGGGCTCATGGAATGGCCTACGCCG 540 243 3313 Antisense
GCGCCGCTCAACGGAAATTCCGGCT 560 287 3357 Antisense
AGCATTGCCAACTCGATTCGGCTCT 85 79 3409 Antisense
Sequence Source Affymetrix Proprietary Database