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[176939_at]

Description Y34D9A.G /REP_DB=WormBase Gene ID /WP=CE21539 /CHR=1 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF59437.1
Cluster 6grp_highest_10_10 > 23      6grp_high1_10_10 > 22      6grp_highest_nonorm > 22      6grp_total > 22      4grp_total > 54      6grp_highest_sub > 01_02_03      6grp_cgc > 71      6grp_high1_sub > De      6grp_cgc_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2748
1 2 3 4 range
A 2611.8
(3)
P 		1328
(0)
P 		1230.1
(-0.2)
P 		6.2
(-3)
A 		2606
B 2753.1
(3)
P 		1109.8
(-0.6)
P 		1253.6
(-0.3)
P 		5.3
(-3)
A 		2748
C 2531.6
(3)
P 		1135.6
(-0.3)
P 		843.2
(-1)
P 		17.4
(-3)
A 		2514
avr 2632.2
(3) 		1191.1
(-0.3) 		1109
(-0.5) 		9.6
(-3) 		2623

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2220
1 2 3 4 5 6 range
A 1713.1
(1.5)
P 		2168
(2.8)
P 		2248.2
(3)
P 		1359.2
(0.4)
P 		400.2
(-2.3)
P 		166.2
(-3)
P 		2082
B 1474.2
(1.3)
P 		1988.1
(3)
P 		1905.7
(2.7)
P 		899.5
(-0.6)
P 		498.5
(-1.9)
P 		181.7
(-3)
P 		1806
C 2373.1
(3)
P 		2072.1
(2.2)
P 		2130.7
(2.3)
P 		964.9
(-0.8)
P 		521.7
(-2)
P 		152.8
(-3)
P 		2220
avr 1853.5
(2.2) 		2076.1
(2.9) 		2094.9
(3) 		1074.5
(-0.2) 		473.5
(-2) 		166.9
(-3) 		1928
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 950
1 2 3 4 range
A 951
(3)
P 		288.3
(-1.2)
P 		321.6
(-1)
P 		0.9
(-3)
A 		950
B 877.9
(3)
P 		274.1
(-1.1)
P 		290.5
(-1)
P 		1.1
(-3)
A 		877
C 838
(3)
P 		326.5
(-0.7)
P 		293.9
(-0.9)
P 		2.9
(-3)
A 		835
avr 889
(3) 		296.3
(-1) 		302
(-1) 		1.6
(-3) 		887

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 839
1 2 3 4 5 6 range
A 315.8
(-0.7)
P 		771.1
(3)
P 		489.6
(0.7)
P 		344.7
(-0.5)
P 		120.8
(-2.3)
P 		40.5
(-3)
P 		731
B 513
(1.5)
P 		661.8
(3)
P 		462.8
(1)
P 		238.3
(-1.3)
P 		202.5
(-1.6)
P 		67.8
(-3)
P 		594
C 879.6
(3)
P 		632.3
(1.2)
P 		542.3
(0.6)
P 		194
(-2)
P 		168.8
(-2.1)
P 		51.1
(-3)
P 		829
avr 569.5
(1.9) 		688.4
(3) 		498.2
(1.2) 		259
(-1.1) 		164
(-2) 		53.1
(-3) 		635
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176939_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18642
Sequence Type Exemplar sequence
Representative Public ID Y34D9A.G NCBI
Target Description Y34D9A.G /REP_DB=WormBase Gene ID /WP=CE21539 /CHR=1 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF59437.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001026570(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001026570 NCBI Caenorhabditis elegans SaPosin-like Protein family member (spp-23) (spp-23) mRNA, complete cds. 11/11 None
SNAP00000009163 ENSEMBL cdna:SNAP chromosome:CEL140:I:1018845:1029486:1 10/11 None
GENEFINDER00000009199 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:1018845:1019159:1 11/11 None
Y34D9A.11 ENSEMBL cdna:known chromosome:CEL140:I:1018845:1019159:1 gene:Y34D9A.11 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_170795 1/11 Cross Hyb Matching Probes None
SNAP00000006757 1/11 Cross Hyb Matching Probes None
GENEFINDER00000006758 1/11 Cross Hyb Matching Probes None
C54G6.5 1/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:1018844-1019159(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol spp-23
Ensembl Y34D9A.11
Entrez Gene 259362 Entrez gene
SwissProt Q6AW11 EMBL-EBI
WormBase CE21539 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_001021741 SaPosin-like Protein family member (spp-23) [Caenorhabditis elegans] gb|AAT39979.1| Hypothetical protein Y34D9A.11 [Caenorhabditis elegans] 6.0E-53
blast CAE74319 Hypothetical protein CBG22030 [Caenorhabditis briggsae] 3.0E-37
blast AAL65782 Saposin-like protein family protein 17 [Caenorhabditis elegans] ref|NP_740777.1| SaPosin-like Protein family member (spp-17) [Caenorhabditis elegans] 5.0E-37
blast NP_001021741 SaPosin-like Protein family member (spp-23) [Caenorhabditis elegans] gb|AAT39979.1| Hypothetical protein Y34D9A.11 [Caenorhabditis elegans] 1.0E-36
Protein Domains
Database ID Description E-Value
Pfam IPR008138 EMBL-EBI Saposin-like type B, 2 7.7E-7
Sequence
Target Sequence 
>C. ELEGANS:176939_AT
atcacaagccactctggcctgtatcacatgtgtggctaccgttaagggtctcgagccgaa
agtgctggccgagggagatgcggtggccgagaaagaggtggccgcgttgtgcatgaaaga
ggcgccgacgccggctgccgagaaggattgtgagcaatatggagatgaggaggccgcgat
tattatcgatttgatcaagaaggacgttccaccaaag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATCACAAGCCACTCTGGCCTGTATC 312 27 81 Antisense
GGCCTGTATCACATGTGTGGCTACC 448 543 96 Antisense
GTGGCTACCGTTAAGGGTCTCGAGC 194 491 112 Antisense
GGGTCTCGAGCCGAAAGTGCTGGCC 22 497 126 Antisense
GAAAGTGCTGGCCGAGGGAGATGCG 112 361 138 Antisense
GCCGAGGGAGATGCGGTGGCCGAGA 320 285 148 Antisense
AGAAAGAGGTGGCCGCGTTGTGCAT 252 73 170 Antisense
TGTGCATGAAAGAGGCGCCGACGCC 243 557 188 Antisense
CGCCGGCTGCCGAGAAGGATTGTGA 134 257 209 Antisense
GATGAGGAGGCCGCGATTATTATCG 329 411 244 Antisense
GATCAAGAAGGACGTTCCACCAAAG 309 421 273 Antisense
Sequence Source Affymetrix Proprietary Database