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[176929_at]

Description Y67D8C.B /REP_DB=WormBase Gene ID /WP=CE26231 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAG23370.1
Cluster 6grp_highest_10_10 > 61      6grp_high1_10_10 > 16      4grp_high_8_8 > 41      6grp_highest_nonorm > 70      6grp_total > 17      4grp_total > 54      6grp_highest_sub > De      6grp_cgc > 13      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 456
1 2 3 4 range
A 551.6
(3)
P 		333.9
(-0.3)
P 		255.4
(-1.5)
P 		156.1
(-3)
M 		396
B 553.6
(3)
P 		386.6
(0.5)
P 		252.4
(-1.5)
P 		156.4
(-3)
P 		397
C 594.1
(3)
P 		312
(-0.7)
P 		293.4
(-1)
P 		137.8
(-3)
P 		456
avr 566.4
(3) 		344.2
(-0.2) 		267.1
(-1.3) 		150.1
(-3) 		416

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 747
1 2 3 4 5 6 range
A 330.5
(0.4)
P 		464.7
(3)
P 		205.3
(-2)
P 		189.2
(-2.4)
P 		156.2
(-3)
P 		231.1
(-1.5)
P 		309
B 387.1
(3)
P 		352.5
(2.1)
P 		221.4
(-1.5)
P 		213.7
(-1.7)
P 		166.1
(-3)
P 		217.1
(-1.6)
P 		221
C 847.8
(3)
P 		315.3
(-1.3)
P 		269.9
(-1.6)
P 		100.9
(-3)
P 		279.3
(-1.6)
P 		194.4
(-2.2)
P 		747
avr 521.8
(3) 		377.5
(0.6) 		232.2
(-1.9) 		167.9
(-3) 		200.5
(-2.4) 		214.2
(-2.2) 		354
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 178
1 2 3 4 range
A 200.8
(3)
P 		72.5
(-1.3)
P 		66.8
(-1.5)
P 		22.8
(-3)
M 		178
B 176.5
(3)
P 		95.5
(-0.4)
P 		58.5
(-1.9)
P 		33.3
(-3)
P 		143
C 196.7
(3)
P 		89.7
(-0.7)
P 		102.3
(-0.3)
P 		23.2
(-3)
P 		174
avr 191.3
(3) 		85.9
(-0.8) 		75.9
(-1.2) 		26.4
(-3) 		165

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 294
1 2 3 4 5 6 range
A 60.9
(-2.2)
P 		165.3
(3)
P 		44.7
(-3)
P 		48
(-2.8)
P 		47.1
(-2.9)
P 		56.3
(-2.4)
P 		121
B 134.7
(3)
P 		117.3
(1.7)
P 		53.8
(-3)
P 		56.6
(-2.8)
P 		67.5
(-2)
P 		80.9
(-1)
P 		81
C 314.2
(3)
P 		96.2
(-1.5)
P 		68.7
(-2)
P 		20.3
(-3)
P 		90.4
(-1.6)
P 		65.1
(-2.1)
P 		294
avr 169.9
(3) 		126.3
(1) 		55.7
(-2.3) 		41.6
(-3) 		68.3
(-1.8) 		67.4
(-1.8) 		128
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176929_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19150
Sequence Type Exemplar sequence
Representative Public ID Y67D8C.B NCBI
Target Description Y67D8C.B /REP_DB=WormBase Gene ID /WP=CE26231 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAG23370.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001028385(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001028385 NCBI Caenorhabditis elegans Membrane Calcium ATPase family member (mca-3) (mca-3) mRNA, complete cds. 9/11 None
SNAP00000001621 ENSEMBL cdna:SNAP chromosome:CEL140:IV:3112047:3120947:-1 9/11 None
GENEFINDER00000001623 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:3112047:3114102:-1 10/11 None
Y67D8C.10b ENSEMBL cdna:known chromosome:CEL140:IV:3111405:3131366:-1 gene:Y67D8C.10 9/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
177089 3 1 NM_001028385 176929_at (9)
NM_067893 176980_at (9)
NM_171299 176980_at (9)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:3112052-3121952(-) 94.47 99.04
Public Domain and Genome References
Ensembl Y67D8C.10
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK68551 Membrane calcium atpase protein 3, isoform b [Caenorhabditis elegans] ref|NP_001023556.1| Membrane Calcium ATPase family member (mca-3) [Caenorhabditis elegans] 0.0
blast CAA09303 calcium ATPase [Caenorhabditis elegans] 0.0
blast AAM97979 Membrane calcium atpase protein 3, isoform c [Caenorhabditis elegans] ref|NP_741352.1| Membrane Calcium ATPase family member (mca-3) [Caenorhabditis elegans] 0.0
blast AAK68551 Membrane calcium atpase protein 3, isoform b [Caenorhabditis elegans] ref|NP_001023556.1| Membrane Calcium ATPase family member (mca-3) [Caenorhabditis elegans] 1.0E-67
Protein Families
Method ID Description E-Value
ec A8A1_HUMAN (Q9Y2Q0) Potential phospholipid-transporting ATPase IA (EC 3.6.3.1) (Chromaffin granule ATPase II) (ATPase class I type 8A member 1) 0.0
ec A1A2_HUMAN (P50993) Sodium/potassium-transporting ATPase alpha-2 chain precursor (EC 3.6.3.9) (Sodium pump 2) (Na+/K+ ATPase 2) 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR006068 EMBL-EBI Cation transporting ATPase, C-terminal 4.5E-18
Pfam IPR008250 EMBL-EBI E1-E2 ATPase-associated region 2.7E-21
Pfam IPR008250 EMBL-EBI E1-E2 ATPase-associated region 2.6E-18
Pfam IPR005834 EMBL-EBI Haloacid dehalogenase-like hydrolase 1.2E-12
Pfam IPR004014 EMBL-EBI Cation transporting ATPase, N-terminal 1.2E-22
Pfam IPR006068 EMBL-EBI Cation transporting ATPase, C-terminal 4.5E-18
Pfam IPR008250 EMBL-EBI E1-E2 ATPase-associated region 2.6E-18
Pfam IPR005834 EMBL-EBI Haloacid dehalogenase-like hydrolase 1.2E-12
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_001023556.1 10 86-108,123-142,334-356,376-398,823-845,855-872,904-926,941-958,970-992,1007-1029
Y67D8C.10b 10 86-108,123-142,334-356,376-398,823-845,855-872,904-926,941-958,970-992,1007-1029
SNAP00000001621 7 32-54,74-96,521-543,602-624,639-656,668-690,705-727
Sequence
Target Sequence 
>C. ELEGANS:176929_AT
gtgtgcgtccactgtgttacattcattgaaaagcgggggcaaaattggaaaatttttagc
taaaagtgacttttttggaatttttgaaaagtctcattatgaaaatcggtatctttgggt
ctttttgagtaaatataacaaattacgagtcgtcaaagcattccgatcatcattagaaga
attcgatgagcgacgatcgatcgcctccacacactcggttcaatcaatgcgtggaggatt
ctggagcggcggtggacggcacacaacgacagccgcttcaccagaaccagccggacaacg
tattgcgatggctgcaatcacgcacaggcaatcgccacagccgtacccatactatcatca
ccagcagccacggactccgtctccacaaccacatattgtggtggagaatggaacatcatc
ggggaataatggacaagcagcgacggcggcggcagcagcaccaaaagccgaaacgaatgg
aaaaaccgaaaaagtaccgctggtgcagcaatggtctccggacacaaattcttcgtatac
tcaaacacagc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTGTGCGTCCACTGTGTTACATTCA 346 487 2952 Antisense
ATCGGTATCTTTGGGTCTTTTTGAG 390 35 3056 Antisense
GAGTCGTCAAAGCATTCCGATCATC 33 401 3098 Antisense
CGATGAGCGACGATCGATCGCCTCC 557 249 3135 Antisense
CACACTCGGTTCAATCAATGCGTGG 488 193 3161 Antisense
AGAACCAGCCGGACAACGTATTGCG 395 71 3234 Antisense
GCTGCAATCACGCACAGGCAATCGC 133 297 3262 Antisense
GCAGCAGCACCAAAAGCCGAAACGA 557 313 3403 Antisense
ACCGCTGGTGCAGCAATGGTCTCCG 660 89 3447 Antisense
TGGTCTCCGGACACAAATTCTTCGT 526 551 3463 Antisense
AATTCTTCGTATACTCAAACACAGC 259 177 3478 Antisense
Sequence Source Affymetrix Proprietary Database