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[176924_at]

Description Y34D9A.E /REP_DB=WormBase Gene ID /WP=CE21529 /CHR=1 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF59435.1
Cluster 6grp_high1_10_10 > 89      4grp_highest_8_8 > 04      4grp_high_8_8 > 37      6grp_total > 89      4grp_total > 34      6grp_cgc > 69      6grp_high1_sub > Cu      6grp_cgc_sub > Da      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 257
1 2 3 4 range
A 295.5
(-3)
P 		303.2
(-2.7)
P 		448.3
(3)
P 		409.4
(1.5)
P 		153
B 216.8
(-3)
P 		461.4
(3)
P 		287.6
(-1.3)
P 		297.3
(-1)
P 		245
C 241.7
(-3)
P 		473.3
(3)
P 		273.7
(-2.2)
P 		322.3
(-0.9)
P 		232
avr 251.3
(-3) 		412.6
(3) 		336.5
(0.2) 		343
(0.4) 		161

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 308
1 2 3 4 5 6 range
A 248.8
(-0.7)
P 		185.9
(-2.6)
P 		173.2
(-3)
P 		200.6
(-2.2)
P 		366.9
(3)
P 		357.3
(2.7)
P 		194
B 281.3
(-0.8)
P 		261.3
(-1.3)
P 		180.1
(-3)
P 		292.4
(-0.6)
P 		340.6
(0.4)
P 		461
(3)
P 		281
C 207.3
(-1.1)
P 		189.1
(-1.7)
P 		152.6
(-3)
A 		271.4
(1.2)
P 		315.4
(2.7)
P 		324.1
(3)
P 		172
avr 245.8
(-0.8) 		212.1
(-1.8) 		168.6
(-3) 		254.8
(-0.6) 		341
(1.9) 		380.8
(3) 		212
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 82
1 2 3 4 range
A 107.6
(2)
P 		65.8
(-2.4)
P 		117.2
(3)
P 		59.9
(-3)
P 		57
B 69.1
(-2.3)
P 		113.9
(3)
P 		66.6
(-2.6)
P 		63.4
(-3)
P 		51
C 80
(-1.1)
P 		136.1
(3)
P 		95.4
(0)
P 		54.3
(-3)
P 		82
avr 85.6
(0.4) 		105.3
(3) 		93.1
(1.4) 		59.2
(-3) 		46

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 134
1 2 3 4 5 6 range
A 45.9
(-2.3)
P 		66.1
(-0.7)
P 		37.7
(-3)
P 		50.9
(-1.9)
P 		110.7
(3)
P 		87
(1.1)
P 		73
B 97.9
(-0.5)
P 		87
(-1)
P 		43.7
(-3)
P 		77.4
(-1.4)
P 		138.4
(1.4)
P 		171.9
(3)
P 		128
C 76.8
(0.3)
P 		57.7
(-1.4)
P 		38.8
(-3)
A 		54.6
(-1.6)
P 		102.1
(2.4)
P 		108.5
(3)
P 		70
avr 73.5
(-0.6) 		70.3
(-0.8) 		40.1
(-3) 		61
(-1.5) 		117.1
(2.6) 		122.5
(3) 		82
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176924_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18627
Sequence Type Exemplar sequence
Representative Public ID Y34D9A.E NCBI
Target Description Y34D9A.E /REP_DB=WormBase Gene ID /WP=CE21529 /CHR=1 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF59435.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058408(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058408 NCBI Caenorhabditis elegans SPindle Defective family member (spd-1) (spd-1) mRNA, complete cds. 11/11 None
SNAP00000009164 ENSEMBL cdna:SNAP chromosome:CEL140:I:1019921:1052164:-1 11/11 None
GENEFINDER00000009195 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:1030865:1031056:-1 11/11 None
Y34D9A.4.2 ENSEMBL cdna:known chromosome:CEL140:I:1030635:1033444:-1 gene:Y34D9A.4 11/11 None
Y34D9A.4.1 ENSEMBL cdna:known chromosome:CEL140:I:1030651:1033438:-1 gene:Y34D9A.4 11/11 None
Y34D9A.4.3 ENSEMBL cdna:known chromosome:CEL140:I:1030651:1033440:-1 gene:Y34D9A.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:1030864-1031056(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol spd-1
Ensembl Y34D9A.4
Entrez Gene 171683 Entrez gene
SwissProt Q6AW14 EMBL-EBI
WormBase CE37061 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAV68307 SPD-1 [Caenorhabditis elegans] ref|NP_490809.2| SPindle Defective family member (spd-1) [Caenorhabditis elegans] gb|AAT81176.1| Spindle defective protein 1 [Caenorhabditis elegans] 0.0
blast NP_490808 Y34D9A.1 [Caenorhabditis elegans] gb|AAK29884.1| Hypothetical protein Y34D9A.1 [Caenorhabditis elegans] 0.0
blast CAE74320 Hypothetical protein CBG22031 [Caenorhabditis briggsae] 0.0
blast CAE74321 Hypothetical protein CBG22032 [Caenorhabditis briggsae] 1.0E-50
Protein Domains
Database ID Description E-Value
Pfam IPR007145 EMBL-EBI MAP65/ASE1 1.4E-7
Pfam IPR007145 EMBL-EBI MAP65/ASE1 1.4E-7
Trans Membrane
ID Number Of Domains Domain Boundaries
SNAP00000009164 1 67-89
Sequence
Target Sequence 
>C. ELEGANS:176924_AT
tcccgccacatccgaaatgatcagcttcatcacaccgaccagaagacaagccgtcggccc
gaaaacctcgtcgccaaaggacacacagccctccacctcctcctcatcccgaacgacgac
tccgatgtcgaaacgggcgatgacgccgtcctc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCCCGCCACATCCGAAATGATCAGC 227 597 22 Antisense
GCCACATCCGAAATGATCAGCTTCA 347 279 26 Antisense
CACATCCGAAATGATCAGCTTCATC 580 195 28 Antisense
AGCTTCATCACACCGACCAGAAGAC 412 79 44 Antisense
CATCACACCGACCAGAAGACAAGCC 500 211 49 Antisense
CGACCAGAAGACAAGCCGTCGGCCC 669 249 57 Antisense
CAGAAGACAAGCCGTCGGCCCGAAA 171 191 61 Antisense
TCCCGAACGACGACTCCGATGTCGA 563 595 128 Antisense
GAACGACGACTCCGATGTCGAAACG 558 345 132 Antisense
GACTCCGATGTCGAAACGGGCGATG 491 373 139 Antisense
CGAAACGGGCGATGACGCCGTCCTC 442 223 150 Antisense
Sequence Source Affymetrix Proprietary Database