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[176923_s_at] actin /// actin /// actin

Description M03F4.2 /REP_DB=WormBase Gene ID /WP=CE12358 /GEN=act-4 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=actin
Cluster 6grp_highest_10_10 > 03      6grp_high1_10_10 > 22      4grp_highest_8_8 > 09      4grp_high_8_8 > 61      6grp_highest_nonorm > 08      6grp_total > 33      4grp_total > 58      6grp_highest_sub > De      6grp_cgc > 70      6grp_high1_sub > De      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 11932
1 2 3 4 range
A 19233.9
(0.7)
P 		23109.2
(2.6)
P 		23815.1
(3)
P 		11883.5
(-3)
P 		11932
B 17288.5
(-0.7)
P 		20623.9
(1.5)
P 		22983.7
(3)
P 		13765.3
(-3)
P 		9218
C 17215.2
(-0.6)
P 		22343.5
(3)
P 		21567.9
(2.5)
P 		13851
(-3)
P 		8493
avr 17912.5
(-0) 		22025.5
(2.5) 		22788.9
(3) 		13166.6
(-3) 		9622

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 7767
1 2 3 4 5 6 range
A 20401.3
(-0.2)
P 		20121.3
(-0.5)
P 		23507.7
(3)
P 		18757.7
(-2)
P 		17769.1
(-3)
P 		18014.5
(-2.7)
P 		5739
B 20291.6
(3)
P 		19327.3
(1.7)
P 		17419.2
(-0.8)
P 		17553
(-0.6)
P 		17170.1
(-1.1)
P 		15740.3
(-3)
P 		4551
C 19523.4
(2.2)
P 		20058.5
(3)
P 		19969.8
(2.9)
P 		16809.1
(-1.9)
P 		19709.6
(2.5)
P 		16048.2
(-3)
P 		4010
avr 20072.1
(2.6) 		19835.7
(2.2) 		20298.9
(3) 		17706.6
(-1.2) 		18216.3
(-0.4) 		16601
(-3) 		3698
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 5778
1 2 3 4 range
A 7003.1
(3)
P 		5016.4
(0.7)
P 		6225.7
(2.1)
P 		1739.5
(-3)
P 		5264
B 5513.2
(3)
P 		5093.6
(2)
P 		5325.9
(2.6)
P 		2933.4
(-3)
P 		2580
C 5698.7
(0.9)
P 		6423.9
(1.7)
P 		7517.1
(3)
P 		2335.5
(-3)
P 		5182
avr 6071.7
(2.6) 		5511.3
(1.7) 		6356.2
(3) 		2336.1
(-3) 		4020

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 3856
1 2 3 4 5 6 range
A 3760.8
(-3)
P 		7156.1
(3)
P 		5119.5
(-0.6)
P 		4757.4
(-1.2)
P 		5361.9
(-0.2)
P 		4386
(-1.9)
P 		3395
B 7060.5
(3)
P 		6433.3
(1.7)
P 		4229.9
(-3)
P 		4649.2
(-2.1)
P 		6976
(2.8)
P 		5869.5
(0.5)
P 		2831
C 7236.5
(3)
P 		6120.5
(1.3)
P 		5082.7
(-0.4)
P 		3380.3
(-3)
P 		6377.7
(1.7)
P 		5372.3
(0.1)
P 		3856
avr 6019.3
(1.6) 		6570
(3) 		4810.7
(-1.6) 		4262.3
(-3) 		6238.5
(2.1) 		5209.3
(-0.5) 		2308
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 176923_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16225
Sequence Type Exemplar sequence
Representative Public ID M03F4.2 NCBI
Target Description M03F4.2 /REP_DB=WormBase Gene ID /WP=CE12358 /GEN=act-4 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=actin
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073416(11), NM_073417(11), NM_073418(11), NM_076440(11), NM_076441(11), NM_001029632(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073416 NCBI Caenorhabditis elegans T04C12.4 (actin) mRNA, complete cds. 11/11 A 
NM_073417 NCBI Caenorhabditis elegans T04C12.5 (actin) mRNA, complete cds. 11/11 A 
NM_073418 NCBI Caenorhabditis elegans T04C12.6 (actin) mRNA, complete cds. 11/11 A 
NM_076440 NCBI Caenorhabditis elegans ACTin family member (act-4) (act-4) mRNA, complete cds. 11/11 A 
NM_076441 NCBI Caenorhabditis elegans ACTin family member (act-4) (act-4) mRNA, complete cds. 11/11 A 
NM_001029632 NCBI Caenorhabditis elegans ACTin family member (act-4) (act-4) mRNA, complete cds. 11/11 None
SNAP00000040054 ENSEMBL cdna:SNAP chromosome:CEL140:V:11074600:11075831:-1 11/11 None
SNAP00000040055 ENSEMBL cdna:SNAP chromosome:CEL140:V:11079488:11080715:-1 11/11 None
SNAP00000040056 ENSEMBL cdna:SNAP chromosome:CEL140:V:11083661:11084891:1 11/11 None
GENEFINDER00000040059 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:11074600:11075831:-1 11/11 None
GENEFINDER00000040060 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:11079488:11080715:-1 11/11 None
GENEFINDER00000040065 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:11083661:11084891:1 11/11 None
GENEFINDER00000026198 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:4960629:4963951:1 11/11 None
SNAP00000026190 ENSEMBL cdna:SNAP chromosome:CEL140:X:4962842:4963951:1 11/11 None
T04C12.4.5 ENSEMBL cdna:known chromosome:CEL140:V:11074084:11077082:-1 gene:T04C12.4 11/11 A 
T04C12.4.6 ENSEMBL cdna:known chromosome:CEL140:V:11074084:11076048:-1 gene:T04C12.4 11/11 A 
T04C12.4.4 ENSEMBL cdna:known chromosome:CEL140:V:11074084:11075857:-1 gene:T04C12.4 11/11 A 
T04C12.4.1 ENSEMBL cdna:known chromosome:CEL140:V:11074084:11075852:-1 gene:T04C12.4 11/11 A 
T04C12.4.2 ENSEMBL cdna:known chromosome:CEL140:V:11074084:11077086:-1 gene:T04C12.4 11/11 A 
T04C12.4.3 ENSEMBL cdna:known chromosome:CEL140:V:11074086:11075852:-1 gene:T04C12.4 11/11 A 
T04C12.5 ENSEMBL cdna:known chromosome:CEL140:V:11078897:11080742:-1 gene:T04C12.5 11/11 A 
T04C12.6.3 ENSEMBL cdna:known chromosome:CEL140:V:11081755:11085086:1 gene:T04C12.6 11/11 A 
T04C12.6.2 ENSEMBL cdna:known chromosome:CEL140:V:11083627:11085086:1 gene:T04C12.6 11/11 A 
T04C12.6.4 ENSEMBL cdna:known chromosome:CEL140:V:11083640:11085184:1 gene:T04C12.6 11/11 A 
T04C12.6.1 ENSEMBL cdna:known chromosome:CEL140:V:11083640:11085086:1 gene:T04C12.6 11/11 A 
M03F4.2a.1 ENSEMBL cdna:known chromosome:CEL140:X:4960520:4964383:1 gene:M03F4.2 11/11 A 
M03F4.2a.2 ENSEMBL cdna:known chromosome:CEL140:X:4960546:4964527:1 gene:M03F4.2 11/11 A 
M03F4.2a.3 ENSEMBL cdna:known chromosome:CEL140:X:4960546:4967028:1 gene:M03F4.2 11/11 A 
M03F4.2b.1 ENSEMBL cdna:known chromosome:CEL140:X:4960586:4963951:1 gene:M03F4.2 11/11 A 
M03F4.2b.2 ENSEMBL cdna:known chromosome:CEL140:X:4960586:4963951:1 gene:M03F4.2 11/11 A 
M03F4.2c ENSEMBL cdna:known chromosome:CEL140:X:4961894:4963951:1 gene:M03F4.2 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:11083660-11084891(+) 94.16 94.16
chrX:4960629-4963952(+) 100.0 100.0
chrV:11074599-11075831(-) 94.16 94.16
chrV:11079487-11080715(-) 91.87 91.87
Public Domain and Genome References
Gene Title actin
actin
actin
Gene Symbol act-3
act-2
act-1
Ensembl T04C12.4
T04C12.5
T04C12.6
M03F4.2
Entrez Gene 179533 Entrez gene
179534 Entrez gene
179535 Entrez gene
SwissProt P10983 EMBL-EBI
P10984 EMBL-EBI
WormBase CE13148 Wormbase
CE13150 Wormbase
CE13148 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:250458_S_AT actin 7 (ACT7) / actin 2 at
ATGENOME1:15697_AT actin 12 (ACT12) at
ATH1-121501:252531_AT actin 12 (ACT12) at
CANINE:1603908_X_AT similar to cytoplasmic beta-actin cfa
CANINE:1582872_X_AT similar to cytoplasmic beta-actin cfa
CANINE_2:CFAAFFX.15835.1.S1_AT similar to cytoplasmic beta-actin cfa
CANINE_2:CFAAFFX.23258.1.S1_X_AT similar to cytoplasmic beta-actin cfa
CANINE_2:CFAAFFX.23260.1.S1_X_AT similar to cytoplasmic beta-actin cfa
CANINE:1603908_X_AT actin, beta cfa
CANINE:1582872_X_AT actin, beta cfa
CANINE_2:CFA.17735.1.S1_AT actin, beta cfa
CANINE_2:CFAAFFX.15835.1.S1_AT actin, beta cfa
CANINE_2:CFAAFFX.23260.1.S1_X_AT actin, beta cfa
CANINE_2:CFAAFFX.24576.1.S1_S_AT actin, beta cfa
CANINE:1582760_X_AT actin, beta cfa
CANINE:1582871_AT actin, beta cfa
CANINE:1589539_S_AT Beta-actin cfa
CANINE:1589540_X_AT Beta-actin cfa
CANINE:1592991_AT Beta-actin cfa
CANINE:1595277_AT Beta-actin cfa
CANINE:1597268_AT Beta-actin cfa
CANINE:1603027_AT Beta-actin cfa
CANINE:1603081_AT Beta-actin cfa
CANINE:1594631_S_AT Beta-actin cfa
CANINE:1603282_AT Beta-actin cfa
CANINE:1603467_AT Beta-actin cfa
CANINE:1595325_X_AT Beta-actin cfa
CANINE:1602497_AT Beta-actin cfa
DROSOPHILA_2:1626163_S_AT Actin 5C dm
DROSGENOME1:143058_F_AT Actin 5C dm
DROSOPHILA_2:1634250_AT Actin 57B dm
DROSGENOME1:153531_AT Actin 57B dm
CHICKEN:GGA.4737.1.S1_AT actin, cytoplasmic, type 5 gga
CHICKEN:GGA.4520.1.S1_S_AT Actin, cytoplasmic, type 5 gga
HG-U133_PLUS_2:210926_AT actin-like protein hs
HG-U133A:210926_AT actin-like protein hs
HG-U133A_2:210926_AT actin-like protein hs
U133_X3P:G12408251_3P_X_AT actin-like protein hs
U133_X3P:G12408251_3P_S_AT actin-like protein hs
U133_X3P:G12408251_3P_AT actin-like protein hs
U133_X3P:210926_3P_AT actin-like protein hs
HG-U133_PLUS_2:213867_X_AT actin, beta hs
HG-U133A_2:213867_X_AT actin, beta hs
HG-U133A:213867_X_AT actin, beta hs
HG-U133_PLUS_2:200801_X_AT actin, beta hs
HG-U133A:200801_X_AT actin, beta hs
HG-U133A_2:200801_X_AT actin, beta hs
HU35KSUBA:V00478_S_AT actin, beta hs
HU35KSUBC:RC_D51245_F_AT actin, beta hs
HUGENEFL:X00351_F_AT actin, beta hs
HUGENEFL:M10277_S_AT actin, beta hs
U133_X3P:G5016088_3P_X_AT actin, beta hs
U133_X3P:G12408251_3P_S_AT actin, beta hs
U133_X3P:HS.288061.2.A1_3P_S_AT actin, beta hs
HG-U133_PLUS_2:224594_X_AT actin, beta hs
HG-U133B:224594_X_AT actin, beta hs
HG-U95AV2:32318_S_AT actin, beta hs
HG-U95C:64355_S_AT actin, beta hs
HG-U95D:74526_AT Actin, beta hs
MG-U74AV2:96573_AT actin, gamma, cytoplasmic 1 mm
MOUSE430_2:1415779_S_AT actin, gamma, cytoplasmic 1 mm
MOUSE430A_2:1415779_S_AT actin, gamma, cytoplasmic 1 mm
MOE430A:1415779_S_AT actin, gamma, cytoplasmic 1 mm
MU11KSUBA:M21495_F_AT actin, gamma, cytoplasmic 1 mm
MU11KSUBB:MSA.42673.0_F_AT actin, gamma, cytoplasmic 1 mm
MU11KSUBB:MSA.12411.0_F_AT actin, gamma, cytoplasmic 1 mm
MU11KSUBB:MSA.401.0_F_AT actin, gamma, cytoplasmic 1 mm
MU19KSUBC:TC39202_F_AT Actin, gamma, cytoplasmic 1, mRNA (cDNA clone MGC:30279 IMAGE:3499390) mm
MG-U74AV2:101578_F_AT actin, beta, cytoplasmic mm
MG-U74AV2:95705_S_AT actin, beta, cytoplasmic mm
MU11KSUBA:J04181_F_AT actin, beta, cytoplasmic mm
MU11KSUBA:AA709861_F_AT actin, beta, cytoplasmic mm
MU11KSUBA:AA590859_F_AT actin, beta, cytoplasmic mm
MU11KSUBB:MSA.17336.0_F_AT actin, beta, cytoplasmic mm
MU11KSUBB:MSA.29918.0_F_AT actin, beta, cytoplasmic mm
MU11KSUBB:MSA.29217.0_F_AT actin, beta, cytoplasmic mm
MU11KSUBB:MSA.28725.0_F_AT actin, beta, cytoplasmic mm
MU11KSUBB:MSA.24085.0_F_AT actin, beta, cytoplasmic mm
MU11KSUBB:MSA.22727.0_F_AT actin, beta, cytoplasmic mm
MOE430A:1436722_A_AT actin, beta, cytoplasmic mm
MOUSE430A_2:1436722_A_AT actin, beta, cytoplasmic mm
MOUSE430_2:1436722_A_AT actin, beta, cytoplasmic mm
MOE430A:1419734_AT actin, beta, cytoplasmic mm
MOUSE430A_2:1419734_AT actin, beta, cytoplasmic mm
MOUSE430_2:1419734_AT actin, beta, cytoplasmic mm
MU11KSUBB:MSA.32904.0_F_AT Actin, beta, cytoplasmic (Actb), mRNA mm
MU11KSUBB:MSA.35112.0_S_AT Actin, beta, cytoplasmic (Actb), mRNA mm
MU19KSUBB:TC26671_F_AT Actin, beta, cytoplasmic (Actb), mRNA mm
MU19KSUBB:TC26672_AT Actin, beta, cytoplasmic (Actb), mRNA mm
MU19KSUBC:TC35527_F_AT Actin, beta, cytoplasmic (Actb), mRNA mm
MU19KSUBC:TC35528_AT Actin, beta, cytoplasmic (Actb), mRNA mm
MU19KSUBC:TC39087_F_AT Actin, beta, cytoplasmic (Actb), mRNA mm
MU19KSUBC:TC39088_AT Actin, beta, cytoplasmic (Actb), mRNA mm
MU19KSUBC:TC39089_I_AT Actin, beta, cytoplasmic (Actb), mRNA mm
MU19KSUBC:TC39089_S_AT Actin, beta, cytoplasmic (Actb), mRNA mm
RG-U34A:RC_AA900769_S_AT actin, gamma, cytoplasmic (predicted) rn
RG-U34A:X52815CDS_F_AT actin, gamma, cytoplasmic (predicted) rn
RG-U34B:RC_AA964496_S_AT actin, gamma, cytoplasmic (predicted) rn
RG-U34C:RC_AI104357_AT actin, gamma, cytoplasmic (predicted) rn
RAE230A:1371327_A_AT actin, gamma, cytoplasmic (predicted) rn
RAT230_2:1371327_A_AT actin, gamma, cytoplasmic (predicted) rn
RAE230B:1384581_AT actin, gamma, cytoplasmic (predicted) rn
RAT230_2:1384581_AT actin, gamma, cytoplasmic (predicted) rn
RT-U34:V01217_AT actin, beta rn
RG-U34A:V01217_AT actin, beta rn
RG-U34A:RC_AI179012_S_AT actin, beta rn
RG-U34A:RC_AI070848_F_AT actin, beta rn
RAE230A:1398835_AT actin, beta rn
RAT230_2:1398835_AT actin, beta rn
RAE230A:1398836_S_AT actin, beta rn
RAT230_2:1398836_S_AT actin, beta rn
RG-U34A:RC_AA874855_AT Actin, beta rn
YEAST_2:1769719_AT Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Sc
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
5884 actin filament inferred from electronic annotation QuickGO AmiGO
15629 actin cytoskeleton inferred from electronic annotation QuickGO AmiGO
5856 cytoskeleton inferred from sequence similarity QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3774 motor activity inferred from electronic annotation QuickGO AmiGO
5200 structural constituent of cytoskeleton inferred from electronic annotation QuickGO AmiGO
5200 structural constituent of cytoskeleton inferred from sequence similarity QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB04678 Hypothetical protein T04C12.6 [Caenorhabditis elegans] emb|CAB04676.1| Hypothetical protein T04C12.4 [Caenorhabditis elegans] ref|NP_505819.1| T04C12.6 [Caenorhabditis elegans] ref|NP_505817.1| T04C12.4 [Caenorhabditis elegans] sp|P10983|ACT1_CAEEL Actin-1/3 emb|CAA34717.1| actin [Caenorhabditis elegans] 0.0
blast AAR21857 actin [Cooperia oncophora] gb|AAB04575.1| Actin protein 4, isoform a [Caenorhabditis elegans] emb|CAA34720.1| actin [Caenorhabditis elegans] ref|NP_508841.1| ACTin family member (act-4) [Caenorhabditis elegans] gb|AAY52453.1| actin [Caenorhabditis remanei] sp|P10986|ACT4_CAEEL Actin-4 emb|CAE68670.1| Hypothetical protein CBG14574 [Caenorhabditis briggsae] emb|CAE75153.1| Hypothetical protein CBG23090 [Caenorhabditis briggsae] 0.0
blast 1D4X Chain , Crystal Structure Of Caenorhabditis Elegans Mg-Atp Actin Complexed With Human Gelsolin Segment 1 At 1.75 A Resolution 0.0
blast CAB04675 Hypothetical protein T04C12.5 [Caenorhabditis elegans] ref|NP_505818.1| T04C12.5 [Caenorhabditis elegans] sp|P10984|ACT2_CAEEL Actin-2 emb|CAE75154.1| Hypothetical protein CBG23091 [Caenorhabditis briggsae] 0.0
blast CAA34718 actin [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR004000 EMBL-EBI Actin/actin-like 1.0E-126
Pfam IPR004000 EMBL-EBI Actin/actin-like 1.0E-126
Pfam IPR004000 EMBL-EBI Actin/actin-like 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:176923_S_AT
tcttccctcgagaagtcctacgaacttcctgacggacaggtcatcaccgttggaaacgag
cgtttccgttgcccagaggctctcttccagccatccttcttgggtatggagtctgccgga
atccacgagacttcttacaactccatcatgaagtgcgacattgatatccgtaaggacttg
tacgccaacaccgtcctttccggaggaaccaccatgtacccaggaatcgccgatcgtatg
cagaaggaaatcaccgctcttgccccatccaccatgaagatcaagatcatcgccccacca
gagcgcaagtactccgtctggatcggaggatccatcctcgcctccctctccaccttccaa
cagatgtggatctccaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCTTCCCTCGAGAAGTCCTACGAAC 271 611 715 Antisense
AGTCCTACGAACTTCCTGACGGACA 156 41 728 Antisense
GGAATCCACGAGACTTCTTACAACT 597 531 832 Antisense
GAGACTTCTTACAACTCCATCATGA 492 391 841 Antisense