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[176917_at]

Description C53D5.1 /REP_DB=WormBase Gene ID /WP=CE23609 /CHR=1 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF39783.1
Cluster 6grp_highest_10_10 > 50      6grp_high1_10_10 > 15      4grp_highest_8_8 > 57      4grp_high_8_8 > 34      6grp_highest_nonorm > 70      6grp_total > 04      4grp_total > 45      6grp_highest_sub > De      6grp_high1_sub > De      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 194
1 2 3 4 range
A 302.8
(3)
P 		257.5
(1.2)
P 		198.6
(-1.2)
P 		155.4
(-3)
P 		147
B 309.8
(3)
P 		270.9
(1.2)
P 		176.8
(-3)
P 		197.8
(-2.1)
P 		133
C 348.9
(3)
P 		256.2
(-0.2)
P 		176.9
(-3)
P 		258.6
(-0.1)
P 		172
avr 320.5
(3) 		261.5
(0.4) 		184.1
(-3) 		203.9
(-2.1) 		136

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 355
1 2 3 4 5 6 range
A 488.2
(3)
P 		258.3
(-0.9)
P 		230.6
(-1.4)
P 		233.3
(-1.3)
P 		259.6
(-0.9)
P 		133.3
(-3)
P 		355
B 382
(2)
P 		413.9
(3)
P 		227.7
(-3)
P 		352
(1)
P 		295.4
(-0.8)
P 		253.3
(-2.2)
P 		186
C 345.7
(1.9)
P 		381.5
(3)
P 		186.3
(-3)
P 		271.7
(-0.4)
P 		205.5
(-2.4)
P 		227.8
(-1.7)
P 		195
avr 405.3
(3) 		351.2
(1.4) 		214.9
(-2.7) 		285.7
(-0.6) 		253.5
(-1.5) 		204.8
(-3) 		201
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 93
1 2 3 4 range
A 110.2
(3)
P 		55.9
(-0.7)
P 		51.9
(-1)
P 		22.8
(-3)
P 		87
B 98.8
(3)
P 		66.9
(-0.3)
P 		41
(-3)
P 		42.1
(-2.9)
P 		58
C 115.5
(3)
P 		73.6
(-0.5)
P 		61.6
(-1.5)
P 		43.6
(-3)
P 		72
avr 108.2
(3) 		65.5
(-0.6) 		51.5
(-1.7) 		36.2
(-3) 		72

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 105
1 2 3 4 5 6 range
A 90
(2.8)
P 		91.9
(3)
P 		50.2
(-1.2)
P 		59.2
(-0.3)
P 		78.3
(1.6)
P 		32.5
(-3)
P 		59
B 132.9
(2.6)
P 		137.8
(3)
P 		55.3
(-3)
P 		93.2
(-0.2)
P 		120
(1.7)
P 		94.4
(-0.2)
P 		83
C 128.1
(3)
P 		116.4
(2.1)
P 		47.4
(-3)
P 		54.6
(-2.5)
P 		66.5
(-1.6)
P 		76.2
(-0.9)
P 		81
avr 117
(3) 		115.4
(2.9) 		51
(-3) 		69
(-1.4) 		88.3
(0.4) 		67.7
(-1.5) 		66
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176917_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2552
Sequence Type Exemplar sequence
Representative Public ID C53D5.1 NCBI
Target Description C53D5.1 /REP_DB=WormBase Gene ID /WP=CE23609 /CHR=1 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF39783.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058311(11), NM_058312(11), NM_182074(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058311 NCBI Caenorhabditis elegans C53D5.1a (C53D5.1) mRNA, complete cds. 11/11 None
NM_058312 NCBI Caenorhabditis elegans C53D5.1b (C53D5.1) mRNA, complete cds. 11/11 None
NM_182074 NCBI Caenorhabditis elegans C53D5.1c (C53D5.1) mRNA, complete cds. 11/11 None
GENEFINDER00000029146 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:291249:305081:1 11/11 None
SNAP00000029142 ENSEMBL cdna:SNAP chromosome:CEL140:I:302672:308375:1 11/11 None
C53D5.1a ENSEMBL cdna:known chromosome:CEL140:I:291216:305461:1 gene:C53D5.1 11/11 A 
C53D5.1b ENSEMBL cdna:known chromosome:CEL140:I:295364:305081:1 gene:C53D5.1 11/11 None
C53D5.1c ENSEMBL cdna:known chromosome:CEL140:I:302596:305081:1 gene:C53D5.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:302671-305081(+) 100.0 100.0
Public Domain and Genome References
Ensembl C53D5.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK68264 Hypothetical protein C53D5.1a [Caenorhabditis elegans] ref|NP_490712.1| C53D5.1a [Caenorhabditis elegans] 0.0
blast AAK68265 Hypothetical protein C53D5.1b [Caenorhabditis elegans] ref|NP_490713.1| C53D5.1b [Caenorhabditis elegans] 0.0
blast AAN84836 Hypothetical protein C53D5.1c [Caenorhabditis elegans] ref|NP_871874.1| C53D5.1c [Caenorhabditis elegans] 0.0
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_490712.1 1 61-80
NP_490713.1 1 59-78
C53D5.1a 1 61-80
C53D5.1b 1 59-78
GENEFINDER00000029146 1 61-80
Sequence
Target Sequence 
>C. ELEGANS:176917_AT
tcgacgatccttgtgggctttgttaaagcagcctgaattcatcctgttccgagtgttacc
gttgacacgtgtcacgccgaagctcattgggacgtgtggacatatgtatcagactgagtc
actggtggcgttcaagatgaaagggtattatacgaatttgaaggcaaaaatcttggtgca
tgtcatgggaaccctgaagctcttatacgaatttctcgacgaaccccttcaatggtgtga
tgttcgatttgataatctcgggttatcagcagattatccgaaaagattcgctctaatgga
cggtgacatggtgtacacgaaatcgaaattggattctctcctaaaaggacgtccgtgtga
aagtgataatgattgtaaaattggagactgtgaggcgagatgcacttctaatatggtgtg
ctcgtcgaggagtaacgggaatttggaggtcttctgcgataaactggtcaacaaactgtt
cgcaaatcaatggtccaaaaataataaatatttggtcgcatgccgggatacaggacgaaa
tatcacaactcgactcaacga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCGACGATCCTTGTGGGCTTTGTTA 32 575 480 Antisense
AAGCAGCCTGAATTCATCCTGTTCC 619 151 505 Antisense
ATCCTGTTCCGAGTGTTACCGTTGA 83 27 520 Antisense
TTACCGTTGACACGTGTCACGCCGA 97 659 535 Antisense
GTCACGCCGAAGCTCATTGGGACGT 574 463 550 Antisense
GACTGAGTCACTGGTGGCGTTCAAG 648 375 591 Antisense
CATGGGAACCCTGAAGCTCTTATAC 80 209 663 Antisense
CTCGACGAACCCCTTCAATGGTGTG 316 233 694 Antisense
GGAGGTCTTCTGCGATAAACTGGTC 338 515 924 Antisense
TTTGGTCGCATGCCGGGATACAGGA 283 667 990 Antisense
GAAATATCACAACTCGACTCAACGA 78 363 1016 Antisense
Sequence Source Affymetrix Proprietary Database