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[176915_at] fatty acid desaturase

Description Y67H2B.A /REP_DB=WormBase Gene ID /WP=CE22790 /CHR=4 /FEA=Sanger Annotation /DEF=(HINXTON) protein_id:CAB60965.1
Cluster 6grp_highest_10_10 > 39      6grp_high1_10_10 > 82      4grp_highest_8_8 > 41      4grp_high_8_8 > 50      6grp_highest_nonorm > 28      6grp_total > 73      4grp_total > 62      6grp_highest_sub > 04      6grp_cgc > 76      6grp_high1_sub > 04      6grp_cgc_sub > 03_04      6grp_total_sub > Hat      6grp_rule_based > S4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2697
1 2 3 4 range
A 7862.6
(3)
P 		6719.1
(-0.2)
P 		6598.4
(-0.5)
P 		5706.4
(-3)
P 		2156
B 6616.6
(1)
P 		6592.8
(0.8)
P 		6967.8
(3)
P 		5921.4
(-3)
P 		1046
C 7516.6
(3)
P 		6262.3
(-0.2)
P 		6426.8
(0.2)
P 		5165.6
(-3)
P 		2351
avr 7331.9
(3) 		6524.7
(0.2) 		6664.3
(0.7) 		5597.8
(-3) 		1734

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 9373
1 2 3 4 5 6 range
A 6088.3
(-3)
P 		6074
(-3)
P 		10790.4
(1.2)
P 		12448.1
(2.7)
P 		12827.2
(3)
P 		8735.6
(-0.6)
P 		6753
B 6778.2
(-3)
P 		7164.1
(-2.6)
P 		11753
(1.6)
P 		13315.7
(3)
P 		12902.5
(2.6)
P 		9476.2
(-0.5)
P 		6538
C 5512.1
(-3)
P 		7432.8
(-1.8)
P 		9526.1
(-0.4)
P 		14884.6
(3)
P 		11071.7
(0.6)
P 		9120.7
(-0.7)
P 		9373
avr 6126.2
(-3) 		6890.3
(-2.4) 		10689.8
(0.7) 		13549.5
(3) 		12267.1
(2) 		9110.8
(-0.6) 		7423
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2028
1 2 3 4 range
A 2862.8
(3)
P 		1458.5
(-1.2)
P 		1724.9
(-0.4)
P 		835.3
(-3)
P 		2028
B 2110
(3)
P 		1628.3
(-0.4)
P 		1614.6
(-0.5)
P 		1261.8
(-3)
P 		848
C 2488.2
(3)
P 		1800.4
(0.4)
P 		2239.9
(2.1)
P 		871
(-3)
P 		1617
avr 2487
(3) 		1629.1
(-0.4) 		1859.8
(0.5) 		989.4
(-3) 		1498

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 4120
1 2 3 4 5 6 range
A 1122.3
(-3)
P 		2160.2
(-0.7)
P 		2349.9
(-0.3)
P 		3157.1
(1.4)
P 		3870.7
(3)
P 		2126.8
(-0.8)
P 		2748
B 2358.5
(-3)
P 		2384.6
(-2.9)
P 		2854
(-2)
P 		3526.9
(-0.6)
P 		5242.1
(3)
P 		3533.6
(-0.6)
P 		2884
C 2043.1
(-3)
P 		2268
(-2.1)
P 		2424.6
(-1.5)
P 		2993.3
(0.7)
P 		3582.6
(3)
P 		3053.2
(0.9)
P 		1540
avr 1841.3
(-3) 		2270.9
(-1.9) 		2542.8
(-1.2) 		3225.8
(0.5) 		4231.8
(3) 		2904.5
(-0.3) 		2391
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176915_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19089
Sequence Type Exemplar sequence
Representative Public ID Y67H2B.A NCBI
Target Description Y67H2B.A /REP_DB=WormBase Gene ID /WP=CE22790 /CHR=4 /FEA=Sanger Annotation /DEF=(HINXTON) protein_id:CAB60965.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001028389(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001028389 NCBI Caenorhabditis elegans FATty acid desaturase family member (fat-1) (fat-1) mRNA, complete cds. 11/11 None
SNAP00000010788 ENSEMBL cdna:SNAP chromosome:CEL140:IV:13314720:13318575:1 11/11 None
GENEFINDER00000010791 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:13315615:13318575:1 11/11 None
Y67H2A.8.2 ENSEMBL cdna:known chromosome:CEL140:IV:13315611:13318752:1 gene:Y67H2A.8 11/11 None
Y67H2A.8.1 ENSEMBL cdna:known chromosome:CEL140:IV:13315613:13318752:1 gene:Y67H2A.8 11/11 None
Y67H2A.8.3 ENSEMBL cdna:known chromosome:CEL140:IV:13315613:13318745:1 gene:Y67H2A.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:13315620-13318581(+) 100.0 100.0
Public Domain and Genome References
Gene Title fatty acid desaturase
Gene Symbol fat-1
Ensembl Y67H2A.8
Entrez Gene 178291 Entrez gene
SwissProt Q9NEQ0 EMBL-EBI
WormBase CE22790 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
16491 oxidoreductase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAC44309 Hypothetical protein Y67H2A.8 [Caenorhabditis elegans] ref|NP_001023560.1| FATty acid desaturase family member (fat-1) [Caenorhabditis elegans] 0.0
blast AAA67369 fatty acid desaturase 0.0
blast CAE58623 Hypothetical protein CBG01791 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR005804 EMBL-EBI Fatty acid desaturase 1.6E-67
Pfam IPR005804 EMBL-EBI Fatty acid desaturase 1.6E-67
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_001023560.1 3 98-120,235-257,261-280
Y67H2A.8.2 3 98-120,235-257,261-280
Y67H2A.8.1 3 98-120,235-257,261-280
Y67H2A.8.3 3 98-120,235-257,261-280
SNAP00000010788 3 111-133,248-270,274-293
GENEFINDER00000010791 3 98-120,235-257,261-280
Sequence
Target Sequence 
>C. ELEGANS:176915_AT
ccatactcttcactttttgttcgtaactctgaacgtgttcaatgtgtaatctctggaatc
tgttgctgtgtgtgtgcatatattgctctaacaattgctggatcatattccaattggttc
tggtactattgggttccactttctttcttcggattgatgctcgtcattgttacctatttg
caacatgtcgatgatgtcgctgaggtgtacgaggctgatgaatggagcttcgtccgtgga
caaacccaaaccatcgatcgttactatggactcggattggacacaacgatgcaccatatc
acagacggacacgttgcccatcacttcttcaacaaaatcccacattaccatctcatcgaa
gcaaccgaaggtgtcaaaaaggtcttggagccgttgtccgacacccaatacgggtacaaa
tctcaagtgaactacgatttctttgcccgtttcctgtggttcaactacaagctcgactat
ctcgttcacaagac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CCATACTCTTCACTTTTTGTTCGTA 616 267 673 Antisense
ATCTCTGGAATCTGTTGCTGTGTGT 460 33 721 Antisense
CCACTTTCTTTCTTCGGATTGATGC 351 275 808 Antisense
GATGCTCGTCATTGTTACCTATTTG 106 415 828 Antisense
GAGCTTCGTCCGTGGACAAACCCAA 426 191 897 Antisense
CAAACCCAAACCATCGATCGTTACT 687 187 913 Antisense
TATCACAGACGGACACGTTGCCCAT 283 659 969 Antisense
CATTACCATCTCATCGAAGCAACCG 554 215 1015 Antisense
GTGAACTACGATTTCTTTGCCCGTT 223 481 1099 Antisense
CCGTTTCCTGTGGTTCAACTACAAG 551 263 1119 Antisense
AGCTCGACTATCTCGTTCACAAGAC 338 79 1142 Antisense
Sequence Source Affymetrix Proprietary Database