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[176901_at]

Description Y73E7A.B /REP_DB=WormBase Gene ID /WP=CE26412 /CHR=1 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAG23384.1
Cluster 6grp_highest_10_10 > 90      6grp_high1_10_10 > 09      4grp_highest_8_8 > 55      4grp_high_8_8 > 19      6grp_highest_nonorm > 81      6grp_total > 19      4grp_total > 21      6grp_cgc > 99      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 184
1 2 3 4 range
A 379.3
(-0.3)
P 		308.2
(-2.6)
P 		297.4
(-3)
P 		477.9
(3)
P 		181
B 404.7
(3)
P 		329
(-1.1)
P 		294.4
(-3)
P 		335.8
(-0.7)
P 		110
C 350.1
(0.5)
P 		371.6
(2.2)
P 		305.8
(-3)
P 		381.3
(3)
P 		76
avr 378
(1.8) 		336.3
(-0.8) 		299.2
(-3) 		398.3
(3) 		99

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 171
1 2 3 4 5 6 range
A 360.8
(2.1)
P 		298.1
(-3)
P 		317.3
(-1.4)
P 		342
(0.6)
P 		372
(3)
P 		313.1
(-1.8)
P 		74
B 383.5
(2.5)
P 		262.2
(-2.1)
P 		255.5
(-2.3)
P 		330.6
(0.5)
P 		396.8
(3)
P 		238.2
(-3)
P 		159
C 290.7
(-0)
P 		230.9
(-2.8)
P 		329.3
(1.8)
P 		226.1
(-3)
P 		356
(3)
P 		282.4
(-0.4)
P 		130
avr 345
(1.4) 		263.7
(-3) 		300.7
(-1) 		299.6
(-1.1) 		374.9
(3) 		277.9
(-2.2) 		111
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 74
1 2 3 4 range
A 138.1
(3)
P 		66.9
(-3)
P 		77.8
(-2.1)
P 		69.9
(-2.7)
P 		71
B 129.1
(3)
P 		81.3
(-1.7)
P 		68.2
(-3)
P 		71.6
(-2.7)
P 		61
C 115.9
(3)
P 		106.9
(2)
P 		106.6
(1.9)
P 		64.3
(-3)
P 		52
avr 127.7
(3) 		85
(-1.3) 		84.2
(-1.4) 		68.6
(-3) 		59

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 116
1 2 3 4 5 6 range
A 66.5
(-3)
P 		106
(2.2)
P 		69.1
(-2.7)
P 		86.8
(-0.3)
P 		112.3
(3)
P 		76.2
(-1.7)
P 		46
B 133.5
(1.3)
P 		87.3
(-1.5)
P 		62
(-3)
P 		87.6
(-1.5)
P 		161.2
(3)
P 		88.8
(-1.4)
P 		99
C 107.7
(2.4)
P 		70.5
(-0.8)
P 		83.8
(0.3)
P 		45.5
(-3)
P 		115.2
(3)
P 		94.5
(1.2)
P 		70
avr 102.6
(0.2) 		87.9
(-1.3) 		71.6
(-3) 		73.3
(-2.8) 		129.6
(3) 		86.5
(-1.5) 		58
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176901_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18761
Sequence Type Exemplar sequence
Representative Public ID Y73E7A.B NCBI
Target Description Y73E7A.B /REP_DB=WormBase Gene ID /WP=CE26412 /CHR=1 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAG23384.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058471(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058471 NCBI Caenorhabditis elegans BT (Bacillus thuringiensis) toxin REsistant family member (bre-4) (bre-4) mRNA, complete cds. 11/11 None
SNAP00000005638 ENSEMBL cdna:SNAP chromosome:CEL140:I:1600625:1626584:-1 11/11 None
GENEFINDER00000005647 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:1621131:1626606:-1 11/11 None
Y73E7A.7 ENSEMBL cdna:known chromosome:CEL140:I:1621527:1626606:-1 gene:Y73E7A.7 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:1621526-1626606(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol bre-4
Ensembl Y73E7A.7
Entrez Gene 190668 Entrez gene
SwissProt Q9GUM2 EMBL-EBI
WormBase CE26412 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1603172_AT similar to UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase 1, membrane-bound form cfa
CANINE_2:CFA.19120.1.S1_AT similar to UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase 1, membrane-bound form cfa
CANINE_2:CFAAFFX.3670.1.S1_AT similar to UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase 1, membrane-bound form cfa
DROSOPHILA_2:1638113_AT dm
DROSGENOME1:152505_AT dm
CHICKEN:GGA.2840.1.S1_AT UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1 gga
HG-U133_PLUS_2:211631_X_AT UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1 hs
HG-U133A:211631_X_AT UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1 hs
HG-U133A_2:211631_X_AT UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1 hs
HG-U133_PLUS_2:216627_S_AT UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1 hs
HG-U133A_2:216627_S_AT UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1 hs
HG-U133A:216627_S_AT UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1 hs
HG-U95AV2:32772_S_AT UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1 hs
HG-U133A:201883_S_AT UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1 hs
HG-U133_PLUS_2:201883_S_AT UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1 hs
HG-U133A_2:201883_S_AT UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1 hs
HG-FOCUS:201883_S_AT UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1 hs
HU35KSUBC:RC_H85120_S_AT UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1 hs
HUGENEFL:X14085_S_AT UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1 hs
HUGENEFL:U10473_S_AT UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1 hs
HUGENEFL:D29805_AT UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1 hs
U133_X3P:G474986_3P_X_AT UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1 hs
U133_X3P:G474986_3P_A_AT UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1 hs
U133_X3P:HS.198248.3.S1_3P_S_AT UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1 hs
U133_X3P:HS.198248.0.S1_3P_S_AT UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1 hs
HG-U133A_2:201882_X_AT UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1 hs
HG-U133_PLUS_2:201882_X_AT UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1 hs
HG-U133A:201882_X_AT UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1 hs
HG-U95AV2:40960_AT UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1 hs
HG-U133_PLUS_2:238987_AT UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1 hs
HG-U133B:238987_AT UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1 hs
U133_X3P:HS.176725.0.S1_3P_AT UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1 hs
HG-U133_PLUS_2:228498_AT UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1 hs
HG-U133B:228498_AT UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1 hs
HG-U133_PLUS_2:229403_AT UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1 hs
HG-U133B:229403_AT UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1 hs
HG-U95C:53643_AT UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1 hs
HG-U95E:67896_I_AT UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1 hs
HG-U95D:83378_AT UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1 hs
HU35KSUBA:AA233236_AT UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1 hs
U133_X3P:HS.198248.1.A1_3P_AT UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1 hs
U133_X3P:HS.198248.2.A1_3P_AT UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1 hs
MU11KSUBA:J03880_S_AT UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1 mm
MG-U74AV2:103002_AT UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1 mm
MOE430A:1418014_A_AT UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1 mm
MOUSE430A_2:1418014_A_AT UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1 mm
MOUSE430_2:1418014_A_AT UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1 mm
MOE430B:1458616_AT UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1 (B4galt1), mRNA mm
MOUSE430_2:1458616_AT UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1 (B4galt1), mRNA mm
MOUSE430_2:1458773_AT UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1 (B4galt1), mRNA mm
MOE430B:1458773_AT UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1 (B4galt1), mRNA mm
MU19KSUBA:TC19549_S_AT UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1 (B4galt1), mRNA mm
RG-U34A:S81025_AT UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1 rn
RG-U34C:RC_AA818683_AT UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1 rn
RAE230A:1375250_AT UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1 rn
RAT230_2:1375250_AT UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1 rn
RAE230A:1371073_AT UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1 rn
RAT230_2:1371073_AT UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1 rn
RAE230A:1375755_AT UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1 rn
RAT230_2:1375755_AT UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1 rn
RG-U34C:RC_AI103162_AT UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1 rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
5975 carbohydrate metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
9045 xylose isomerase activity inferred from electronic annotation QuickGO AmiGO
16757 transferase activity, transferring glycosyl groups inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAG23384 Bt (bacillus thuringiensis) toxin resistant protein 4 [Caenorhabditis elegans] ref|NP_490872.1| BT (Bacillus thuringiensis) toxin REsistant family member (bre-4) [Caenorhabditis elegans] gb|AAS21308.1| BRE-4 [Caenorhabditis elegans] gb|AAM95168.1| UDPGalNAc:GlcNAc{beta}-R {beta}1,4-N-acetylgalactosaminyltransferase [Caenorhabditis elegans] 0.0
blast CAE74432 Hypothetical protein CBG22165 [Caenorhabditis briggsae] 0.0
blast AAG23386 Hypothetical protein Y73E7A.5 [Caenorhabditis elegans] ref|NP_490869.1| Y73E7A.5 [Caenorhabditis elegans] 1.0E-110
blast XP_394839 PREDICTED: similar to ENSANGP00000010877, partial [Apis mellifera] 6.0E-82
Protein Domains
Database ID Description E-Value
Pfam IPR003859 EMBL-EBI Metazoa galactosyltransferase 1.0E-126
Pfam IPR009598 EMBL-EBI Bladder cancer-related BC10 2.5E-29
Pfam IPR003859 EMBL-EBI Metazoa galactosyltransferase 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_490872.1 1 7-29
Y73E7A.7 1 7-29
SNAP00000005638 1 7-29
GENEFINDER00000005647 2 7-29,443-462
Sequence
Target Sequence 
>C. ELEGANS:176901_AT
tacccatggcagtgcttcatctttcatgatgtcgatttactgcccgaagatgaccgtaac
ctgtacacgtgtccaattcaaccacgtcatatgagtgtagcgatcgataaattcaattat
aaacttccatattcggcgatcttcggcggaatcagtgcactaacaaaagatcacctgaag
aaaatcaatggattttcgaatgatttttggggttggggcggagaggacgacgatttggcg
acgagaacatcgatggctggactgaaagtttcaagatatccgacacaaattgcacgatat
aaaatgattaagcactcgacggaagcgacgaatccagttaataaatgccgctacaaaata
atgggccaaacgaagcgccgatggacacgtgacggcctaagcaatctgaagtataagctc
gtaaatctggaattgaagcctctctacactcgagccgtcgtcgatttgctcgaaaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TACCCATGGCAGTGCTTCATCTTTC 502 649 643 Antisense
CATGATGTCGATTTACTGCCCGAAG 635 205 667 Antisense
TGCCCGAAGATGACCGTAACCTGTA 635 583 683 Antisense
GTAACCTGTACACGTGTCCAATTCA 585 461 698 Antisense
TAAACTTCCATATTCGGCGATCTTC 309 641 762 Antisense
CGATCTTCGGCGGAATCAGTGCACT 411 249 779 Antisense
GGAGAGGACGACGATTTGGCGACGA 192 519 862 Antisense
AACGAAGCGCCGATGGACACGTGAC 305 145 1011 Antisense
GACACGTGACGGCCTAAGCAATCTG 541 367 1026 Antisense
TCTGGAATTGAAGCCTCTCTACACT 623 607 1068 Antisense
GAGCCGTCGTCGATTTGCTCGAAAA 577 383 1094 Antisense
Sequence Source Affymetrix Proprietary Database