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[176900_at]

Description Y23H5B.C /REP_DB=WormBase Gene ID /WP=CE25231 /CHR=1 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF60456.1
Cluster 6grp_total > 58      4grp_total > 22      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 256
1 2 3 4 range
A 421
(3)
P 		220.4
(-1.8)
A 		171.3
(-3)
A 		404.4
(2.6)
P 		250
B 326.9
(1.1)
P 		188.4
(-2.5)
A 		167
(-3)
M 		403.4
(3)
P 		236
C 358.8
(2.5)
P 		247.4
(-0.7)
A 		165.5
(-3)
P 		377.4
(3)
P 		212
avr 368.9
(2.3) 		218.7
(-1.7) 		167.9
(-3) 		395.1
(3) 		227

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 191
1 2 3 4 5 6 range
A 197.4
(-0.5)
A 		181.3
(-1.1)
A 		189.2
(-0.8)
P 		131.4
(-2.9)
A 		127.7
(-3)
P 		294.9
(3)
P 		167
B 266.2
(2.3)
P 		103.6
(-3)
P 		223.7
(0.9)
P 		118.1
(-2.5)
A 		155.6
(-1.3)
P 		286.3
(3)
P 		183
C 169.9
(-0.8)
P 		150.2
(-1.6)
P 		113.4
(-3)
A 		151.5
(-1.5)
M 		203.8
(0.5)
P 		268.3
(3)
P 		155
avr 211.2
(0.1) 		145
(-2.5) 		175.4
(-1.3) 		133.7
(-3) 		162.4
(-1.8) 		283.2
(3) 		150
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 115
1 2 3 4 range
A 153.3
(3)
P 		47.8
(-2.8)
A 		44.8
(-3)
A 		59.2
(-2.2)
P 		109
B 104.2
(3)
P 		46.5
(-2.3)
A 		38.7
(-3)
M 		86
(1.3)
P 		66
C 118.8
(3)
P 		71.1
(-1.7)
A 		57.7
(-3)
P 		63.6
(-2.4)
P 		61
avr 125.4
(3) 		55.1
(-2.4) 		47.1
(-3) 		69.6
(-1.3) 		78

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 78
1 2 3 4 5 6 range
A 36.4
(-2.5)
A 		64.5
(1.9)
A 		41.2
(-1.8)
P 		33.3
(-3)
A 		38.5
(-2.2)
P 		71.8
(3)
P 		39
B 92.6
(1.9)
P 		34.5
(-2.7)
P 		54.3
(-1.2)
P 		31.3
(-3)
A 		63.2
(-0.5)
P 		106.8
(3)
P 		76
C 63
(0.4)
P 		45.8
(-1.3)
P 		28.9
(-3)
A 		30.5
(-2.8)
M 		66
(0.7)
P 		89.8
(3)
P 		61
avr 64
(0.4) 		48.3
(-1.3) 		41.5
(-2) 		31.7
(-3) 		55.9
(-0.5) 		89.5
(3) 		58
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176900_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18729
Sequence Type Exemplar sequence
Representative Public ID Y23H5B.C NCBI
Target Description Y23H5B.C /REP_DB=WormBase Gene ID /WP=CE25231 /CHR=1 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF60456.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058588(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058588 NCBI Caenorhabditis elegans Y23H5B.6 (Y23H5B.6) mRNA, complete cds. 11/11 None
SNAP00000000925 ENSEMBL cdna:SNAP chromosome:CEL140:I:2489985:2497262:1 11/11 None
GENEFINDER00000000940 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:2489985:2497262:1 11/11 None
Y23H5B.6 ENSEMBL cdna:known chromosome:CEL140:I:2489977:2497372:1 gene:Y23H5B.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:2489984-2497262(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y23H5B.6
Entrez Gene 171810 Entrez gene
SwissProt Q9N478 EMBL-EBI
WormBase CE25231 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:248136_AT DEAD/DEAH box helicase, putative at
CANINE_2:CFAAFFX.22117.1.S1_AT similar to DEAD (Asp-Glu-Ala-Asp) box polypeptide 10 cfa
CANINE_2:CFAAFFX.22122.1.S1_S_AT similar to DEAD (Asp-Glu-Ala-Asp) box polypeptide 10 cfa
DROSOPHILA_2:1624138_AT dm
DROSGENOME1:145251_AT dm
HG-U95AV2:831_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 10 hs
HC-G110:831_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 10 hs
HG-U133_PLUS_2:204977_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 10 hs
HG-FOCUS:204977_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 10 hs
HG-U133A_2:204977_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 10 hs
HG-U133A:204977_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 10 hs
HU35KSUBA:HUM_ALU_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 10 hs
HU35KSUBB:HUM_ALU_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 10 hs
HU35KSUBC:HUM_ALU_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 10 hs
HU35KSUBD:HUM_ALU_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 10 hs
HUGENEFL:HUM_ALU_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 10 hs
HC-G110:HUM_ALU_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 10 hs
HUGENEFL:U28042_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 10 hs
U133_X3P:G13514830_3P_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 10 hs
U133_X3P:HS2.376837.1.S1_3P_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 10 hs
HG-U133_PLUS_2:1563522_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 10 hs
HG-U95D:74247_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 10 hs
RG-U34C:RC_AI177039_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 10 (predicted) rn
RAE230A:1373549_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 10 (predicted) rn
RAT230_2:1373549_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 10 (predicted) rn
YEAST_2:1773231_AT Putative nucleolar DEAD box RNA helicase; high-copy number suppression of a U14 snoRNA processing mutant suggests an involvement in 18S rRNA synthesis Sc
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
3676 nucleic acid binding inferred from electronic annotation QuickGO AmiGO
4386 helicase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
8026 ATP-dependent helicase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAF60456 Hypothetical protein Y23H5B.6 [Caenorhabditis elegans] ref|NP_490989.1| Y23H5B.6 [Caenorhabditis elegans] 0.0
blast CAE60391 Hypothetical protein CBG03992 [Caenorhabditis briggsae] 0.0
blast AAH96386 LOC613047 protein [Xenopus tropicalis] 1.0E-142
Protein Domains
Database ID Description E-Value
Pfam IPR001650 EMBL-EBI Helicase, C-terminal 7.8E-23
Pfam IPR001410 EMBL-EBI DEAD/DEAH box helicase 1.4E-73
Sequence
Target Sequence 
>C. ELEGANS:176900_AT
aagcctacaggagagcctgagcagatcaaggaagaagaagccgctgaaaagatctcaaat
atcaccatttccaagacgaaaccgctcaaaaaagcagtgacaaaagtgtcggcggcgaag
aaaatcctcaacaaaaagcttaaagttaacacgaaaaagacatttggagcagaagatgat
gagctcgaagagctcgaagaaggcccctcaactatcacctcctatggtttggatattgaa
aaagcccggcaggagatcaaaagtggcgataaggaggatcggaaaaggttcaagctgctc
cgcgagcaacggagacaggagaagcttgcgaagaagaatcggagagctgaggaggaagaa
gctgtggagtatgatgaggatggaagtgaggatgagccggatatctcgtggctcccggat
ccggatgcggttagaaggaagtatgaggaggagccggatgaggtggaag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAGCCTACAGGAGAGCCTGAGCAGA 687 147 1702 Antisense
CACCATTTCCAAGACGAAACCGCTC 368 199 1764 Antisense
GTCGGCGGCGAAGAAAATCCTCAAC 706 463 1809 Antisense
CGAAGAGCTCGAAGAAGGCCCCTCA 324 251 1887 Antisense
TCGAAGAAGGCCCCTCAACTATCAC 313 601 1895 Antisense
CAACTATCACCTCCTATGGTTTGGA 565 187 1910 Antisense
CGGAAAAGGTTCAAGCTGCTCCGCG 247 243 1981 Antisense
AACGGAGACAGGAGAAGCTTGCGAA 12 143 2009 Antisense
ATGAGCCGGATATCTCGTGGCTCCC 540 45 2093 Antisense
GCTCCCGGATCCGGATGCGGTTAGA 577 297 2112 Antisense
GAGGAGGAGCCGGATGAGGTGGAAG 626 403 2146 Antisense
Sequence Source Affymetrix Proprietary Database