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[176891_at]

Description Y23H5B.G /REP_DB=WormBase Gene ID /WP=CE23008 /CHR=1 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF60460.1
Cluster 6grp_total > 75      4grp_total > 18      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 43
1 2 3 4 range
A 13.2
(3)
A 		9.7
(1.3)
A 		1
(-3)
A 		3.9
(-1.6)
A 		12
B 3.8
(-3)
A 		19.9
(3)
A 		4.9
(-2.6)
A 		6.1
(-2.1)
A 		16
C 6
(-3)
A 		5.7
(-3)
A 		21.1
(-0.6)
A 		43.6
(3)
A 		38
avr 7.7
(-3) 		11.8
(-0.6) 		9
(-2.2) 		17.9
(3) 		10

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 48
1 2 3 4 5 6 range
A 1.2
(-3)
A 		25
(-0)
A 		20.2
(-0.6)
A 		49.5
(3)
A 		6.3
(-2.4)
A 		8.3
(-2.1)
A 		48
B 1.4
(-2.8)
A 		7.5
(2.2)
A 		1.5
(-2.8)
A 		1.2
(-3)
A 		1.8
(-2.5)
A 		8.5
(3)
A 		7
C 2.3
(-2.8)
A 		1.3
(-3)
A 		1.6
(-3)
A 		2.3
(-2.8)
A 		3.2
(-2.7)
A 		38.1
(3)
A 		37
avr 1.6
(-3) 		11.3
(0.5) 		7.8
(-0.8) 		17.7
(2.8) 		3.8
(-2.2) 		18.3
(3) 		17
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 7
1 2 3 4 range
A 4.8
(3)
A 		2.1
(-0.6)
A 		0.3
(-3)
A 		0.6
(-2.6)
A 		5
B 1.2
(-2.8)
A 		4.9
(3)
A 		1.1
(-3)
A 		1.3
(-2.7)
A 		4
C 2
(-2.6)
A 		1.6
(-3)
A 		7.4
(3)
A 		7.4
(3)
A 		6
avr 2.7
(-3) 		2.9
(-0) 		2.9
(-0) 		3.1
(3) 		0

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 13
1 2 3 4 5 6 range
A 0.2
(-3)
A 		8.9
(1.2)
A 		4.4
(-1)
A 		12.5
(3)
A 		1.9
(-2.2)
A 		2
(-2.1)
A 		12
B 0.5
(-2.6)
A 		2.5
(1.6)
A 		0.4
(-2.8)
A 		0.3
(-3)
A 		0.8
(-2)
A 		3.2
(3)
A 		3
C 0.9
(-2.8)
A 		0.4
(-3)
A 		0.4
(-3)
A 		0.5
(-3)
A 		1
(-2.7)
A 		12.8
(3)
A 		12
avr 0.5
(-3) 		3.9
(0.7) 		1.7
(-1.7) 		4.4
(1.3) 		1.2
(-2.2) 		6
(3) 		6
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176891_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18691
Sequence Type Exemplar sequence
Representative Public ID Y23H5B.G NCBI
Target Description Y23H5B.G /REP_DB=WormBase Gene ID /WP=CE23008 /CHR=1 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF60460.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058594(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058594 NCBI Caenorhabditis elegans Y23H5B.2 (Y23H5B.2) mRNA, complete cds. 11/11 A 
GENEFINDER00000000943 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:2549297:2550544:1 9/11 None
Y23H5B.2 ENSEMBL cdna:known chromosome:CEL140:I:2549297:2550199:1 gene:Y23H5B.2 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000000932 6/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:2549296-2550199(+) 100.0 100.0
chrI:2331280-2332601(-) 91.19 91.19
Public Domain and Genome References
Gene Title
Ensembl Y23H5B.2
Entrez Gene 189525 Entrez gene
SwissProt Q9N473 EMBL-EBI
WormBase CE23008 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAF60460 Hypothetical protein Y23H5B.2 [Caenorhabditis elegans] ref|NP_490995.1| Y23H5B.2 [Caenorhabditis elegans] 2.0E-21
blast NP_490964 Y39G10AR.16 [Caenorhabditis elegans] gb|AAK39603.1| Hypothetical protein Y39G10AR.16 [Caenorhabditis elegans] 2.0E-14
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_490995.1 1 10-32
Y23H5B.2 1 10-32
GENEFINDER00000000943 1 10-32
Sequence
Target Sequence 
>C. ELEGANS:176891_AT
ccatggtgccatgaattacctgtgtttcgctgctgctgcgtcaatttctggagcgatcat
tgccgtcggctgtaccaccggacatcgtgagaattcgcgtggtggcaaaggaaaatcggc
gagatcaaagcgatcgaaacgatcaaaacgagacaagaagcaaaactcgaagagacaaca
aaactcgaaacgacaaaagaagattaagagcaattctgccgctccggcaccaccggccaa
gagtggagtgcctgcaaaatcggcggagaaggcaaaggaaggatcgcagagatcggctcg
actcaagaaggagcagaagtccgagaagaagaaggtttgtgtagatttacggagtacagt
gtata

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CCATGGTGCCATGAATTACCTGTGT 375 271 36 Antisense
GTGCCATGAATTACCTGTGTTTCGC 627 477 41 Antisense
ATGAATTACCTGTGTTTCGCTGCTG 319 43 46 Antisense
GCTGTACCACCGGACATCGTGAGAA 618 293 104 Antisense
ACCGGACATCGTGAGAATTCGCGTG 186 87 112 Antisense
CATCGTGAGAATTCGCGTGGTGGCA 60 213 118 Antisense
GAGAATTCGCGTGGTGGCAAAGGAA 531 389 124 Antisense
TGGTGGCAAAGGAAAATCGGCGAGA 443 551 135 Antisense
AGGATCGCAGAGATCGGCTCGACTC 556 55 315 Antisense
GAAGAAGAAGGTTTGTGTAGATTTA 312 337 360 Antisense
GTAGATTTACGGAGTACAGTGTATA 131 451 376 Antisense
Sequence Source Affymetrix Proprietary Database