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[176887_at]

Description Y73E7A.G /REP_DB=WormBase Gene ID /WP=CE26417 /CHR=1 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAG23388.1
Cluster 6grp_highest_10_10 > 74      6grp_high1_10_10 > 47      4grp_highest_8_8 > 21      4grp_high_8_8 > 23      6grp_highest_nonorm > 90      6grp_total > 46      4grp_total > 0      6grp_highest_sub > Cu      6grp_high1_sub > Cu      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 238
1 2 3 4 range
A 170.8
(-2.8)
P 		162.3
(-3)
P 		236.4
(-1.1)
P 		399.9
(3)
P 		238
B 170.6
(-3)
P 		176.8
(-2.5)
P 		219
(0.9)
P 		244.4
(3)
P 		74
C 220.9
(-0.7)
P 		179.6
(-3)
P 		280.2
(2.5)
P 		288.8
(3)
P 		109
avr 187.4
(-2.4) 		172.9
(-3) 		245.2
(0.1) 		311
(3) 		138

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 133
1 2 3 4 5 6 range
A 138.5
(-0.9)
P 		171.3
(2.8)
P 		149.9
(0.4)
P 		120.2
(-3)
P 		150.4
(0.4)
P 		173.2
(3)
P 		53
B 220.5
(3)
P 		173.8
(0.9)
P 		140.4
(-0.6)
P 		127.8
(-1.2)
P 		87.1
(-3)
P 		205.3
(2.3)
P 		133
C 194.5
(3)
P 		130
(-2.5)
P 		193
(2.9)
P 		165.2
(0.5)
P 		124.7
(-3)
P 		194.7
(3)
P 		70
avr 184.5
(2.4) 		158.4
(0.2) 		161.1
(0.4) 		137.7
(-1.6) 		120.7
(-3) 		191.1
(3) 		70
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 62
1 2 3 4 range
A 62.2
(3)
P 		35.2
(-3)
P 		61.8
(2.9)
P 		58.5
(2.2)
P 		27
B 54.4
(3)
P 		43.7
(-3)
P 		50.8
(1)
P 		52.1
(1.7)
P 		11
C 73.1
(-0)
P 		51.6
(-2.6)
P 		97.6
(3)
P 		48.7
(-3)
P 		49
avr 63.2
(1.4) 		43.5
(-3) 		70.1
(3) 		53.1
(-0.8) 		27

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 51
1 2 3 4 5 6 range
A 25.5
(-3)
P 		60.9
(3)
P 		32.6
(-1.8)
P 		30.5
(-2.2)
P 		45.4
(0.4)
P 		42.2
(-0.2)
P 		35
B 76.7
(3)
P 		57.8
(0.4)
P 		34.1
(-3)
P 		33.9
(-3)
P 		35.4
(-2.8)
P 		76.6
(3)
P 		43
C 72.1
(3)
P 		39.7
(-2)
P 		49.1
(-0.5)
P 		33.2
(-3)
P 		40.3
(-1.9)
P 		65.2
(1.9)
P 		39
avr 58.1
(2.3) 		52.8
(1.2) 		38.6
(-1.7) 		32.5
(-3) 		40.4
(-1.4) 		61.3
(3) 		29
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176887_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18667
Sequence Type Exemplar sequence
Representative Public ID Y73E7A.G NCBI
Target Description Y73E7A.G /REP_DB=WormBase Gene ID /WP=CE26417 /CHR=1 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAG23388.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058469(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058469 NCBI Caenorhabditis elegans Y73E7A.2 (Y73E7A.2) mRNA, complete cds. 9/11 None
GENEFINDER00000005649 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:1601841:1606773:1 9/11 None
Y73E7A.2 ENSEMBL cdna:known chromosome:CEL140:I:1601841:1606773:1 gene:Y73E7A.2 9/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000005639 5/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:1601840-1606773(+) 80.01 100.0
Public Domain and Genome References
Gene Title
Ensembl Y73E7A.2
Entrez Gene 171723 Entrez gene
SwissProt Q9GUM5 EMBL-EBI
WormBase CE32277 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAG23388 Hypothetical protein Y73E7A.2 [Caenorhabditis elegans] ref|NP_490870.2| Y73E7A.2 [Caenorhabditis elegans] 0.0
blast AAG23388 Hypothetical protein Y73E7A.2 [Caenorhabditis elegans] ref|NP_490870.2| Y73E7A.2 [Caenorhabditis elegans] 1.0E-118
blast CAE74425 Hypothetical protein CBG22158 [Caenorhabditis briggsae] 1.0E-100
blast CAE74425 Hypothetical protein CBG22158 [Caenorhabditis briggsae] 3.0E-69
blast EAA04259 ENSANGP00000017925 [Anopheles gambiae str. PEST] ref|XP_308438.2| ENSANGP00000017925 [Anopheles gambiae str. PEST] 4.0E-22
blast XP_760655 hypothetical protein UM04508.1 [Ustilago maydis 521] gb|EAK85390.1| hypothetical protein UM04508.1 [Ustilago maydis 521] 1.0E-11
Sequence
Target Sequence 
>C. ELEGANS:176887_AT
tgccgctttgcctttctgaaaaaaacgttctcgccgacgaggagaagctgctcaaaaaag
tgcgcacaaatcgaagcggaaaaccgcgaatcggcggcaaatccgactctccggaatccc
tggaccccgagacgttcgacgattcggatttctatcaaattctgctcaaacaaatgctcg
aagcacgaaaccagatgagcaacaactctcaagacggagcagacatgacgagaagctata
tggagttacagacaatgttcagtaacaagaaaaaacgagatgtctcgcaactttcgtcaa
aagatcgccgcttgaagtacgagccaatcgcccggctcatcaatttctatccatcgaagc
cgtcggtcgtcacttggagccatgaga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGCCGCTTTGCCTTTCTGAAAAAAA 198 585 1457 Antisense
AAACGTTCTCGCCGACGAGGAGAAG 407 123 1479 Antisense
CGAATCGGCGGCAAATCCGACTCTC 546 253 1543 Antisense
GACCCCGAGACGTTCGACGATTCGG 551 251 1579 Antisense
TCGGATTTCTATCAAATTCTGCTCA 112 607 1600 Antisense
GAAGCACGAAACCAGATGAGCAACA 5 341 1636 Antisense
GAGCAACAACTCTCAAGACGGAGCA 608 389 1653 Antisense
AGATGTCTCGCAACTTTCGTCAAAA 666 67 1734 Antisense
GATCGCCGCTTGAAGTACGAGCCAA 455 417 1759 Antisense
GGCTCATCAATTTCTATCCATCGAA 631 537 1790 Antisense
TCGGTCGTCACTTGGAGCCATGAGA 80 605 1819 Antisense
Sequence Source Affymetrix Proprietary Database