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[176885_at]

Description T27F7.2 /REP_DB=WormBase Gene ID /WP=CE07558 /CHR=2 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAG01562.1
Cluster 6grp_highest_10_10 > 90      6grp_high1_10_10 > 19      4grp_highest_8_8 > 56      4grp_high_8_8 > 27      6grp_highest_nonorm > 40      6grp_total > 29      4grp_total > 37      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 292
1 2 3 4 range
A 791.5
(3)
P 		647.6
(-1.6)
P 		603.2
(-3)
P 		704.2
(0.2)
P 		188
B 828.3
(3)
P 		718.6
(0.7)
P 		536.6
(-3)
P 		577.1
(-2.2)
P 		292
C 755.7
(1.6)
P 		815.6
(3)
P 		567.7
(-3)
P 		739.5
(1.2)
P 		248
avr 791.8
(3) 		727.3
(1.3) 		569.2
(-3) 		673.6
(-0.2) 		223

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 639
1 2 3 4 5 6 range
A 994.2
(3)
P 		768.4
(0.9)
P 		354.8
(-3)
P 		526.7
(-1.4)
P 		828.2
(1.4)
P 		533.7
(-1.3)
P 		639
B 961
(3)
P 		646.8
(-0.4)
P 		404.6
(-3)
P 		611.3
(-0.8)
P 		823.5
(1.5)
P 		518.6
(-1.8)
P 		556
C 817.1
(2.6)
P 		784.4
(2.2)
P 		409.7
(-3)
P 		483.9
(-2)
P 		842.7
(3)
P 		550.2
(-1.1)
P 		433
avr 924.1
(3) 		733.2
(0.9) 		389.7
(-3) 		540.6
(-1.3) 		831.5
(2) 		534.2
(-1.4) 		534
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 185
1 2 3 4 range
A 288.2
(3)
P 		140.6
(-1.8)
P 		157.7
(-1.2)
P 		103.1
(-3)
P 		185
B 264.1
(3)
P 		177.5
(-0.7)
P 		124.3
(-2.9)
P 		123
(-3)
P 		141
C 250.2
(3)
P 		234.5
(2.2)
P 		197.9
(0.5)
P 		124.7
(-3)
P 		126
avr 267.5
(3) 		184.2
(-0.3) 		160
(-1.3) 		116.9
(-3) 		151

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 257
1 2 3 4 5 6 range
A 183.3
(0.2)
P 		273.3
(3)
P 		77.3
(-3)
P 		133.6
(-1.3)
P 		249.9
(2.3)
P 		129.9
(-1.4)
P 		196
B 334.4
(3)
P 		215.3
(-0)
P 		98.2
(-3)
P 		161.9
(-1.4)
P 		334.6
(3)
P 		193.4
(-0.6)
P 		236
C 302.8
(3)
P 		239.3
(1.1)
P 		104.3
(-2.8)
P 		97.3
(-3)
P 		272.7
(2.1)
P 		184.2
(-0.5)
P 		206
avr 273.5
(2.6) 		242.6
(1.7) 		93.3
(-3) 		130.9
(-1.8) 		285.7
(3) 		169.2
(-0.6) 		192
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176885_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4948
Sequence Type Exemplar sequence
Representative Public ID T27F7.2 NCBI
Target Description T27F7.2 /REP_DB=WormBase Gene ID /WP=CE07558 /CHR=2 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAG01562.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062515(11), NM_062514(11), NM_170986(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062515 NCBI Caenorhabditis elegans T27F7.2b (T27F7.2) mRNA, complete cds. 11/11 None
NM_062514 NCBI Caenorhabditis elegans T27F7.2a (T27F7.2) mRNA, complete cds. 11/11 None
NM_170986 NCBI Caenorhabditis elegans T27F7.2c (T27F7.2) mRNA, complete cds. 9/11 None
SNAP00000006640 ENSEMBL cdna:SNAP chromosome:CEL140:II:4987027:4988712:1 11/11 None
GENEFINDER00000006645 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:4982902:4990583:1 11/11 None
T27F7.2b.2 ENSEMBL cdna:known chromosome:CEL140:II:4969187:4988712:1 gene:T27F7.2 11/11 None
T27F7.2a ENSEMBL cdna:known chromosome:CEL140:II:4972492:4988910:1 gene:T27F7.2 11/11 None
T27F7.2b.1 ENSEMBL cdna:known chromosome:CEL140:II:4972839:4988712:1 gene:T27F7.2 11/11 None
T27F7.2c ENSEMBL cdna:known chromosome:CEL140:II:4983464:4988887:1 gene:T27F7.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:4972739-4988710(+) 100.0 100.0
Public Domain and Genome References
Ensembl T27F7.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_494916 T27F7.2b [Caenorhabditis elegans] gb|AAK39302.1| Hypothetical protein T27F7.2b [Caenorhabditis elegans] 0.0
blast NP_494915 T27F7.2a [Caenorhabditis elegans] gb|AAK39301.1| Hypothetical protein T27F7.2a [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000980 EMBL-EBI SH2 motif 1.2E-7
Pfam IPR000980 EMBL-EBI SH2 motif 1.2E-7
Pfam IPR000719 EMBL-EBI Protein kinase 1.5E-4
Pfam IPR000980 EMBL-EBI SH2 motif 1.2E-7
Pfam IPR000980 EMBL-EBI SH2 motif 1.2E-7
Pfam IPR000980 EMBL-EBI SH2 motif 1.2E-7
Sequence
Target Sequence 
>C. ELEGANS:176885_AT
atctatccgatcgatcaggctttgatgacgcaacttgacaattgcagctattttgtggga
caacctacaaaggattcaatggtctacaatctgaatacacaaccggaaggtgcatttgtg
attcgatattcggaaagtaaatcgaaatgtttggcactttcaatgcgtgttccgtgcagt
cacaatccatcgggaatctcacattatcttattatcagaaatgagcaaggattccgattg
aagctctccgccacaaaaaaaccattcccaactcttcaaatgatgctcacacatcactcg
gttctcgaaagtcatctaccgtgtacgcttcactttgtccaatggcaaaagacaaatttt
aagcaattggcggctatgagcacagttgaacggccgctcaggacattcaatcgtcattcg
acagcattaacgaagattgacgagaatcgaaacgtaaccgccacctcaacaccgtaccgt
ccatctggaagat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATCTATCCGATCGATCAGGCTTTGA 386 29 1609 Antisense
TGGCACTTTCAATGCGTGTTCCGTG 492 545 1760 Antisense
GTTCCGTGCAGTCACAATCCATCGG 588 441 1777 Antisense
AATCCATCGGGAATCTCACATTATC 614 167 1792 Antisense
AAGGATTCCGATTGAAGCTCTCCGC 164 161 1835 Antisense
TCACACATCACTCGGTTCTCGAAAG 117 629 1895 Antisense
GAAAGTCATCTACCGTGTACGCTTC 148 361 1915 Antisense
TACGCTTCACTTTGTCCAATGGCAA 401 649 1932 Antisense
GCCGCTCAGGACATTCAATCGTCAT 595 285 2001 Antisense
GAGAATCGAAACGTAACCGCCACCT 543 391 2050 Antisense
ACACCGTACCGTCCATCTGGAAGAT 485 109 2077 Antisense
Sequence Source Affymetrix Proprietary Database