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[176878_at]

Description Y104H12D.3 /REP_DB=WormBase Gene ID /WP=CE26034 /GB=AAK68403.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 44      4grp_total > 03      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 63
1 2 3 4 range
A 34.8
(-3)
A 		35.3
(-2.9)
A 		62.7
(0.7)
A 		80
(3)
A 		45
B 49.9
(-0.8)
A 		29.9
(-3)
A 		38.5
(-2)
A 		84.2
(3)
A 		54
C 50.1
(0.9)
A 		66
(3)
A 		21.6
(-3)
A 		43.5
(-0)
A 		44
avr 44.9
(-2.2) 		43.7
(-2.4) 		40.9
(-3) 		69.2
(3) 		28

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 54
1 2 3 4 5 6 range
A 3.3
(-3)
A 		5.2
(-2.3)
A 		17.2
(2)
A 		9.3
(-0.9)
A 		8.6
(-1.1)
A 		20.1
(3)
A 		17
B 37.9
(0.4)
A 		38.5
(0.5)
A 		54.4
(3)
A 		23.9
(-1.8)
A 		16.4
(-3)
A 		28.6
(-1.1)
A 		38
C 35.2
(0.5)
A 		57.1
(3)
P 		51.7
(2.4)
A 		4.8
(-3)
A 		31
(0)
A 		23
(-0.9)
A 		52
avr 25.5
(-0.3) 		33.6
(1.4) 		41.1
(3) 		12.7
(-3) 		18.7
(-1.7) 		23.9
(-0.6) 		28
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 12
1 2 3 4 range
A 12.7
(0.4)
A 		7.7
(-3)
A 		16.4
(3)
A 		11.7
(-0.2)
A 		9
B 15.9
(1.9)
A 		7.4
(-3)
A 		8.9
(-2.1)
A 		17.9
(3)
A 		11
C 16.6
(1.8)
A 		19
(3)
A 		7.5
(-2.9)
A 		7.3
(-3)
A 		12
avr 15.1
(3) 		11.4
(-2.3) 		10.9
(-3) 		12.3
(-1) 		4

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 17
1 2 3 4 5 6 range
A 0.6
(-3)
A 		1.8
(-1.3)
A 		3.8
(1.5)
A 		2.4
(-0.5)
A 		2.6
(-0.2)
A 		4.9
(3)
A 		4
B 13.2
(3)
A 		12.8
(2.7)
A 		13.2
(3)
A 		6.3
(-3)
A 		6.7
(-2.7)
A 		10.7
(0.8)
A 		7
C 13.1
(1.4)
A 		17.4
(3)
P 		13.2
(1.5)
A 		1
(-3)
A 		10
(0.3)
A 		7.7
(-0.5)
A 		16
avr 9
(1.6) 		10.7
(3) 		10.1
(2.5) 		3.2
(-3) 		6.4
(-0.4) 		7.8
(0.7) 		8
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176878_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10614
Sequence Type Exemplar sequence
Representative Public ID Y104H12D.3 NCBI
Target Description Y104H12D.3 /REP_DB=WormBase Gene ID /WP=CE26034 /GB=AAK68403.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067650(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067650 NCBI Caenorhabditis elegans Y104H12D.3 (Y104H12D.3) mRNA, complete cds. 11/11 None
SNAP00000032195 ENSEMBL cdna:SNAP chromosome:CEL140:IV:1182975:1201418:1 11/11 None
Y104H12D.3 ENSEMBL cdna:known chromosome:CEL140:IV:1193076:1201846:1 gene:Y104H12D.3 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000032199 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:1195122-1201455(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y104H12D.3
Entrez Gene 190868 Entrez gene
SwissProt Q966E7 EMBL-EBI
WormBase CE31632 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6520 amino acid metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
16831 carboxy-lyase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK68403 Hypothetical protein Y104H12D.3 [Caenorhabditis elegans] ref|NP_500051.2| Y104H12D.3 [Caenorhabditis elegans] 0.0
blast CAE68261 Hypothetical protein CBG13938 [Caenorhabditis briggsae] 1.0E-118
blast XP_694140 PREDICTED: similar to sphingosine phosphate lyase 1 [Danio rerio] ref|XP_693742.1| PREDICTED: similar to sphingosine phosphate lyase 1 [Danio rerio] 1.0E-102
blast CAE65555 Hypothetical protein CBG10544 [Caenorhabditis briggsae] 2.0E-96
blast AAC69001 Sphingosine phosphate lyase protein 1 [Caenorhabditis elegans] ref|NP_499913.1| Sphingosine Phosphate Lyase family member (spl-1) [Caenorhabditis elegans] gb|AAD44756.1| sphingosine-1-phosphate lyase [Caenorhabditis elegans] sp|Q9Y194|SGPL_CAEEL Sphingosine-1-phosphate lyase (SP-lyase) (SPL) (Sphingosine-1-phosphate aldolase) 6.0E-96
Protein Domains
Database ID Description E-Value
Pfam IPR002129 EMBL-EBI Pyridoxal-dependent decarboxylase 0.48
Pfam IPR002129 EMBL-EBI Pyridoxal-dependent decarboxylase 2.9E-5
Pfam IPR002129 EMBL-EBI Pyridoxal-dependent decarboxylase 0.48
Pfam IPR002129 EMBL-EBI Pyridoxal-dependent decarboxylase 2.9E-5
Trans Membrane
ID Number Of Domains Domain Boundaries
SNAP00000032195 1 245-264
Sequence
Target Sequence 
>C. ELEGANS:176878_AT
actgcgtgggctatgatgcttcgaaaaggacgagatggatacatcaacgcggctcaacgg
atcattgaggcgacccggcagctcgcctaccgcctacaaggccttgatggggtctcgttg
aggggctccgctgatctgtgtgtggtggcgttcacgacgagcgaagttaatgtgtacaat
ttggtggatgttatggtgcaaaagggttggcacgtggatccactgctttcgccagctgcc
gcccgtgtaccgatctcactttcaatgtgtgaagagggagttttggatcatttcctggag
gatttggaaatggcgatttgcaatttgaagtcaatggagcctgctgagaagctcggaacc
accgcatcgttctaccaaatgctccagaaagtcaatgataaaacgctcgtcgacgagctt
tcactgatccgcctcgccgcccattatagtattccaccgcctgccgagagaagaagtttg
agaactctttccgtcgaaggccgtaaactgagcatgctgg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACTGCGTGGGCTATGATGCTTCGAA 270 99 1048 Antisense
TCAACGCGGCTCAACGGATCATTGA 487 631 1091 Antisense
TACAAGGCCTTGATGGGGTCTCGTT 411 641 1142 Antisense
ATCTGTGTGTGGTGGCGTTCACGAC 245 29 1181 Antisense
AAAGGGTTGGCACGTGGATCCACTG 539 121 1248 Antisense
CGTGTACCGATCTCACTTTCAATGT 330 245 1291 Antisense
CCACCGCATCGTTCTACCAAATGCT 607 273 1406 Antisense
GCTCGTCGACGAGCTTTCACTGATC 602 291 1452 Antisense
TCCACCGCCTGCCGAGAGAAGAAGT 365 587 1500 Antisense
GTTTGAGAACTCTTTCCGTCGAAGG 385 449 1523 Antisense
GAAGGCCGTAAACTGAGCATGCTGG 144 333 1543 Antisense
Sequence Source Affymetrix Proprietary Database