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[176875_at] Phosphoprotein

Description ZK370.5 /REP_DB=WormBase Gene ID /WP=CE00397 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=Phosphoprotein
Cluster 6grp_highest_10_10 > 58      6grp_high1_10_10 > 84      4grp_highest_8_8 > 46      4grp_high_8_8 > 02      6grp_highest_nonorm > 74      6grp_total > 75      4grp_total > 14      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 247
1 2 3 4 range
A 837.6
(1.3)
P 		689.6
(-2.3)
P 		660.9
(-3)
P 		907.6
(3)
P 		247
B 766.9
(1.5)
P 		667.2
(-3)
P 		703.9
(-1.3)
P 		800.2
(3)
P 		133
C 818.9
(1)
P 		683.6
(-3)
P 		735.6
(-1.4)
P 		884.1
(3)
P 		201
avr 807.8
(1.2) 		680.1
(-3) 		700.1
(-2.3) 		864
(3) 		184

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 438
1 2 3 4 5 6 range
A 501
(-3)
P 		640.6
(-0.9)
P 		730.1
(0.4)
P 		760.1
(0.8)
P 		735.5
(0.5)
P 		904.9
(3)
P 		404
B 466.8
(-3)
P 		574.2
(-1.5)
P 		705.1
(0.4)
P 		792.5
(1.6)
P 		631
(-0.7)
P 		887.4
(3)
P 		421
C 565
(-3)
P 		612
(-1.9)
P 		705.8
(0.2)
P 		826.7
(3)
P 		736.3
(0.9)
P 		801.6
(2.4)
P 		262
avr 510.9
(-3) 		608.9
(-1.3) 		713.7
(0.4) 		793.1
(1.8) 		700.9
(0.2) 		864.6
(3) 		354
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 172
1 2 3 4 range
A 305
(3)
P 		149.7
(-2.4)
P 		172.8
(-1.6)
P 		132.9
(-3)
P 		172
B 244.6
(3)
P 		164.8
(-2.9)
P 		163.1
(-3)
P 		170.5
(-2.5)
P 		82
C 271.1
(3)
P 		196.5
(-0.7)
P 		256.4
(2.3)
P 		149.1
(-3)
P 		122
avr 273.6
(3) 		170.3
(-2) 		197.4
(-0.7) 		150.8
(-3) 		123

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 239
1 2 3 4 5 6 range
A 92.4
(-3)
P 		227.8
(3)
P 		159
(-0)
P 		192.8
(1.4)
P 		222
(2.7)
P 		220.3
(2.7)
P 		135
B 162.4
(-3)
P 		191.1
(-2)
P 		171.2
(-2.7)
P 		209.9
(-1.3)
P 		256.4
(0.3)
P 		330.9
(3)
P 		169
C 209.4
(-0.5)
P 		186.8
(-1.8)
P 		179.6
(-2.2)
P 		166.2
(-3)
P 		238.2
(1.2)
P 		268.3
(3)
P 		102
avr 154.7
(-3) 		201.9
(-0.6) 		169.9
(-2.2) 		189.6
(-1.2) 		238.9
(1.3) 		273.2
(3) 		119
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176875_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6876
Sequence Type Exemplar sequence
Representative Public ID ZK370.5 NCBI
Target Description ZK370.5 /REP_DB=WormBase Gene ID /WP=CE00397 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=Phosphoprotein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066527(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066527 NCBI Caenorhabditis elegans ZK370.5 (Phosphoprotein) mRNA, complete cds. 11/11 None
SNAP00000004296 ENSEMBL cdna:SNAP chromosome:CEL140:III:8737945:8739068:-1 9/11 None
GENEFINDER00000004307 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:8737945:8743742:-1 11/11 None
ZK370.5.1 ENSEMBL cdna:known chromosome:CEL140:III:8737587:8741314:-1 gene:ZK370.5 11/11 None
ZK370.5.2 ENSEMBL cdna:known chromosome:CEL140:III:8737587:8741316:-1 gene:ZK370.5 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000004297 2/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:8737940-8741310(-) 100.0 100.0
Public Domain and Genome References
Gene Title Phosphoprotein
Ensembl ZK370.5
Entrez Gene 176226 Entrez gene
SwissProt Q02332 EMBL-EBI
WormBase CE00397 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:258902_AT pyruvate dehydrogenase (lipoamide) kinase (PDHK) at
CANINE_2:CFA.17199.1.S1_AT similar to pyruvate dehydrogenase kinase, isoenzyme 3 cfa
CANINE_2:CFAAFFX.20834.1.S1_S_AT similar to pyruvate dehydrogenase kinase, isoenzyme 3 cfa
DROSOPHILA_2:1629515_AT Pyruvate dehydrogenase kinase dm
CHICKEN:GGAAFFX.10450.1.S1_S_AT pyruvate dehydrogenase kinase, isoenzyme 3 gga
HG-U95AV2:36718_S_AT pyruvate dehydrogenase kinase, isoenzyme 3 hs
HG-U133_PLUS_2:206348_S_AT pyruvate dehydrogenase kinase, isoenzyme 3 hs
HG-U133A:206348_S_AT pyruvate dehydrogenase kinase, isoenzyme 3 hs
HG-U133A_2:206348_S_AT pyruvate dehydrogenase kinase, isoenzyme 3 hs
HG-U133_PLUS_2:206347_AT pyruvate dehydrogenase kinase, isoenzyme 3 hs
HG-U133A:206347_AT pyruvate dehydrogenase kinase, isoenzyme 3 hs
HG-U133A_2:206347_AT pyruvate dehydrogenase kinase, isoenzyme 3 hs
HUGENEFL:L42452_AT pyruvate dehydrogenase kinase, isoenzyme 3 hs
U133_X3P:G4885544_3P_AT pyruvate dehydrogenase kinase, isoenzyme 3 hs
HG-U95AV2:36719_R_AT pyruvate dehydrogenase kinase, isoenzyme 3 hs
HG-U133_PLUS_2:221957_AT Pyruvate dehydrogenase kinase, isoenzyme 3 hs
HG-U133A_2:221957_AT Pyruvate dehydrogenase kinase, isoenzyme 3 hs
HG-FOCUS:221957_AT Pyruvate dehydrogenase kinase, isoenzyme 3 hs
HG-U133A:221957_AT Pyruvate dehydrogenase kinase, isoenzyme 3 hs
U133_X3P:HS.193124.2.S1_3P_AT Pyruvate dehydrogenase kinase, isoenzyme 3 hs
HG-U95AV2:36720_AT Pyruvate dehydrogenase kinase, isoenzyme 3 hs
HG-U95E:90797_AT Pyruvate dehydrogenase kinase, isoenzyme 3 hs
HG-U95D:71907_AT Pyruvate dehydrogenase kinase, isoenzyme 3 hs
MG-U74AV2:92810_AT pyruvate dehydrogenase kinase, isoenzyme 3 mm
MG-U74CV2:171550_AT pyruvate dehydrogenase kinase, isoenzyme 3 mm
MOE430A:1426410_AT pyruvate dehydrogenase kinase, isoenzyme 3 mm
MOUSE430A_2:1426410_AT pyruvate dehydrogenase kinase, isoenzyme 3 mm
MOUSE430_2:1426410_AT pyruvate dehydrogenase kinase, isoenzyme 3 mm
MU19KSUBA:TC24942_AT Pyruvate dehydrogenase kinase, isoenzyme 3 (Pdk3), mRNA mm
RAE230B:1384315_AT pyruvate dehydrogenase kinase, isoenzyme 3 rn
RAT230_2:1384315_AT pyruvate dehydrogenase kinase, isoenzyme 3 rn
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA28207 Hypothetical protein ZK370.5 [Caenorhabditis elegans] ref|NP_498928.1| ZK370.5 [Caenorhabditis elegans] sp|Q02332|PDK_CAEEL Probable [pyruvate dehydrogenase [lipoamide]] kinase, mitochondrial precursor (Pyruvate dehydrogenase kinase) 0.0
blast CAE62763 Hypothetical protein CBG06929 [Caenorhabditis briggsae] 0.0
blast O02623 [Pyruvate dehydrogenase [lipoamide]] kinase, mitochondrial precursor (Pyruvate dehydrogenase kinase) gb|AAB52573.1| pyruvate dehydrogenase kinase [Ascaris suum] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR003594 EMBL-EBI ATP-binding region, ATPase-like 8.3E-19
Pfam IPR003594 EMBL-EBI ATP-binding region, ATPase-like 1.7E-11
Pfam IPR003594 EMBL-EBI ATP-binding region, ATPase-like 8.3E-19
Sequence
Target Sequence 
>C. ELEGANS:176875_AT
atatcgattgtcgccgtaccaagtcatctctatcacatgatgttcgaactgtttaagaat
gcaatgagagcgacagttgaatatcatggagttgatgatgatcttccagatattaaagtt
tatgtagtcaaaggacaagaggatttatctattaagatttgtgatcgtggaggaggtgta
tccagaactattctcgaacgtctctacaactacatgtactcaacagctccaccaccgcca
cgtgatggaacacaagctcctttggctggttacggatacggactgccactatctcgacta
tacgctcggtatttccttggtgacctctttttggtttccatggaaggacatggaacggat
gcatgtatctacttgaaagctgtaccagttgaggctagtgaagtactcccaatttatagt
acttcttcgcgtcgaaatttgacaatgggaccacaagtagctgattggtcgcatcatgtt
cct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATATCGATTGTCGCCGTACCAAGTC 294 19 706 Antisense
CGTACCAAGTCATCTCTATCACATG 459 247 720 Antisense
TATTCTCGAACGTCTCTACAACTAC 607 663 894 Antisense
AACTACATGTACTCAACAGCTCCAC 219 141 913 Antisense
GGAACACAAGCTCCTTTGGCTGGTT 664 527 952 Antisense
GCTGGTTACGGATACGGACTGCCAC 402 291 970 Antisense
CCACTATCTCGACTATACGCTCGGT 284 271 991 Antisense
TACGCTCGGTATTTCCTTGGTGACC 49 645 1006 Antisense
TGGTGACCTCTTTTTGGTTTCCATG 483 551 1023 Antisense
AATTTATAGTACTTCTTCGCGTCGA 321 173 1116 Antisense
AGCTGATTGGTCGCATCATGTTCCT 58 81 1164 Antisense
Sequence Source Affymetrix Proprietary Database