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[176871_at]

Description T08B2.5 /REP_DB=WormBase Gene ID /WP=CE25984 /CHR=1 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAG00022.1
Cluster 6grp_highest_10_10 > 64      6grp_high1_10_10 > 47      4grp_highest_8_8 > 43      4grp_high_8_8 > 24      6grp_highest_nonorm > 74      6grp_total > 46      4grp_total > 39      6grp_highest_sub > Cu      6grp_high1_sub > Cu      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 407
1 2 3 4 range
A 696.1
(3)
P 		451.2
(-2.7)
P 		437.5
(-3)
P 		562
(-0.1)
P 		259
B 815.3
(3)
P 		435.1
(-3)
P 		541.2
(-1.3)
P 		572.6
(-0.8)
P 		380
C 842.5
(3)
P 		438
(-3)
P 		538.1
(-1.5)
P 		503.2
(-2)
P 		405
avr 784.6
(3) 		441.4
(-3) 		505.6
(-1.9) 		545.9
(-1.2) 		343

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 516
1 2 3 4 5 6 range
A 716.6
(0.4)
P 		820.1
(3)
P 		577.6
(-3)
P 		577.3
(-3)
P 		585.2
(-2.8)
P 		705
(0.2)
P 		243
B 916.3
(3)
P 		784.2
(0.8)
P 		688.2
(-0.8)
P 		551.9
(-3)
P 		662.1
(-1.2)
P 		829.4
(1.6)
P 		364
C 704
(0.5)
P 		764.9
(1.2)
P 		568.7
(-1)
P 		607.9
(-0.6)
P 		399.9
(-3)
P 		915.6
(3)
P 		516
avr 779
(2.2) 		789.7
(2.4) 		611.5
(-1.6) 		579
(-2.3) 		549.1
(-3) 		816.7
(3) 		268
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 197
1 2 3 4 range
A 253.4
(3)
P 		97.9
(-2.5)
P 		114.4
(-1.9)
P 		82.3
(-3)
P 		171
B 260
(3)
P 		107.5
(-3)
P 		125.4
(-2.3)
P 		122
(-2.4)
P 		153
C 278.9
(3)
P 		125.9
(-1.7)
P 		187.6
(0.2)
P 		84.8
(-3)
P 		194
avr 264.1
(3) 		110.4
(-2.5) 		142.5
(-1.4) 		96.4
(-3) 		168

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 197
1 2 3 4 5 6 range
A 132.1
(-2.8)
P 		291.7
(3)
P 		125.8
(-3)
P 		146.4
(-2.3)
P 		176.6
(-1.2)
P 		171.7
(-1.3)
P 		166
B 318.8
(3)
P 		261
(1)
P 		167.1
(-2.3)
P 		146.2
(-3)
P 		269
(1.3)
P 		309.3
(2.7)
P 		173
C 260.9
(1.5)
P 		233.4
(0.6)
P 		144.7
(-2.3)
P 		122.3
(-3)
P 		129.4
(-2.8)
P 		306.5
(3)
P 		184
avr 237.3
(1.8) 		262
(3) 		145.9
(-2.6) 		138.3
(-3) 		191.7
(-0.4) 		262.5
(3) 		124
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176871_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1912
Sequence Type Exemplar sequence
Representative Public ID T08B2.5 NCBI
Target Description T08B2.5 /REP_DB=WormBase Gene ID /WP=CE25984 /CHR=1 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAG00022.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059392(11), NM_059393(11), NM_170877(11), NM_182022(11), NM_182021(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059392 NCBI Caenorhabditis elegans T08B2.5b (T08B2.5) mRNA, complete cds. 11/11 None
NM_059393 NCBI Caenorhabditis elegans T08B2.5a (T08B2.5) mRNA, complete cds. 11/11 None
NM_170877 NCBI Caenorhabditis elegans T08B2.5c (T08B2.5) mRNA, complete cds. 11/11 None
NM_182022 NCBI Caenorhabditis elegans T08B2.5g (T08B2.5) mRNA, complete cds. 11/11 None
NM_182021 NCBI Caenorhabditis elegans T08B2.5f (T08B2.5) mRNA, complete cds. 11/11 None
GENEFINDER00000035016 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:6222800:6227972:1 11/11 None
SNAP00000035004 ENSEMBL cdna:SNAP chromosome:CEL140:I:6223926:6227972:1 11/11 None
T08B2.5c ENSEMBL cdna:known chromosome:CEL140:I:6223804:6227972:1 gene:T08B2.5 11/11 None
T08B2.5b ENSEMBL cdna:known chromosome:CEL140:I:6223922:6227972:1 gene:T08B2.5 11/11 None
T08B2.5a ENSEMBL cdna:known chromosome:CEL140:I:6223926:6228036:1 gene:T08B2.5 11/11 None
T08B2.5g ENSEMBL cdna:known chromosome:CEL140:I:6224395:6227972:1 gene:T08B2.5 11/11 None
T08B2.5f ENSEMBL cdna:known chromosome:CEL140:I:6224519:6227972:1 gene:T08B2.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
172312 7 5 NM_059392 176871_at (11)
NM_059393 176871_at (11)
NM_170877 176871_at (11)
NM_182021 176871_at (11)
NM_182022 176871_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:6223854-6227901(+) 100.0 100.0
Public Domain and Genome References
Ensembl T08B2.5
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_871821 T08B2.5f [Caenorhabditis elegans] gb|AAO21392.1| Hypothetical protein T08B2.5f [Caenorhabditis elegans] 0.0
blast NP_740866 T08B2.5c [Caenorhabditis elegans] gb|AAL77184.1| Hypothetical protein T08B2.5c [Caenorhabditis elegans] 0.0
blast NP_491793 T08B2.5b [Caenorhabditis elegans] gb|AAK21463.1| Hypothetical protein T08B2.5b [Caenorhabditis elegans] 0.0
blast NP_871822 T08B2.5g [Caenorhabditis elegans] gb|AAO21393.1| Hypothetical protein T08B2.5g [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000467 EMBL-EBI D111/G-patch domain 2.0E-18
Pfam IPR001876 EMBL-EBI Zn-finger, Ran-binding 1.3E-5
Pfam IPR000467 EMBL-EBI D111/G-patch domain 2.0E-18
Pfam IPR001876 EMBL-EBI Zn-finger, Ran-binding 1.3E-5
Pfam IPR000467 EMBL-EBI D111/G-patch domain 2.0E-18
Pfam IPR001876 EMBL-EBI Zn-finger, Ran-binding 1.3E-5
Pfam IPR000467 EMBL-EBI D111/G-patch domain 2.0E-18
Pfam IPR001876 EMBL-EBI Zn-finger, Ran-binding 1.3E-5
Pfam IPR000467 EMBL-EBI D111/G-patch domain 2.0E-18
Pfam IPR001876 EMBL-EBI Zn-finger, Ran-binding 1.3E-5
Pfam IPR000467 EMBL-EBI D111/G-patch domain 2.0E-18
Pfam IPR001876 EMBL-EBI Zn-finger, Ran-binding 1.3E-5
Pfam IPR000467 EMBL-EBI D111/G-patch domain 2.0E-18
Pfam IPR001876 EMBL-EBI Zn-finger, Ran-binding 1.3E-5
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000035016 1 71-90
Sequence
Target Sequence 
>C. ELEGANS:176871_AT
ctcctcggaaatccactcttcaagatgttagagatgctatggaaagagcactttacgatg
aaacgaagaaaacatgtatgctctgcaaacgagcattctctgatgttgaagtacttcgga
aacatgttgaaaaaagtgatttgcatagaaacaatttggaagcgaagcgagccgaatggg
gacgagaaacagctgctaaattacaagaagaagaagaagatgcttcagctccagatctcc
ctaagatcgtttacagggacagagccaaagaacgtcgtcgtcagttcgggattgattcca
ctggttatgcattcgatgtgatgggtgggccgccaggacccacatcggtgagacatgaag
agaccatccgaaaagttagtgaggaagcttcgaagcgcccattagacgaatcaaacatcg
gaaatagactgctgaaaagtatgggctggaaagaaggacaaggagttggaaagcatgcac
aaggtattgttaacccgattgaagctgaacgatttgttcaaggagcaggtttgggtgctg
ctggatccaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTCCTCGGAAATCCACTCTTCAAGA 381 233 2162 Antisense
GCAAACGAGCATTCTCTGATGTTGA 174 319 2246 Antisense
GCGAAGCGAGCCGAATGGGGACGAG 459 287 2323 Antisense
AGCTCCAGATCTCCCTAAGATCGTT 236 81 2388 Antisense
ACAGAGCCAAAGAACGTCGTCGTCA 416 107 2420 Antisense
GTCGTCGTCAGTTCGGGATTGATTC 53 473 2435 Antisense
GATTCCACTGGTTATGCATTCGATG 610 433 2455 Antisense
GCATTCGATGTGATGGGTGGGCCGC 427 307 2470 Antisense
CCAGGACCCACATCGGTGAGACATG 571 271 2494 Antisense
AAGCTTCGAAGCGCCCATTAGACGA 174 151 2546 Antisense
CAGGTTTGGGTGCTGCTGGATCCAA 400 207 2687 Antisense
Sequence Source Affymetrix Proprietary Database