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[176868_at]

Description ZK616.I /REP_DB=WormBase Gene ID /WP=CE26351 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF60949.1
Cluster 6grp_total > 95      4grp_total > 09      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 196
1 2 3 4 range
A 111.5
(-3)
A 		145.3
(-1.8)
P 		189.7
(-0.1)
A 		275.1
(3)
A 		164
B 196.6
(3)
A 		139.9
(-2)
A 		128.6
(-3)
A 		191.6
(2.6)
A 		68
C 78.7
(-3)
A 		120.6
(-1.1)
A 		110.8
(-1.5)
A 		208.6
(3)
A 		130
avr 128.9
(-3) 		135.3
(-2.6) 		143
(-2.1) 		225.1
(3) 		96

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 230
1 2 3 4 5 6 range
A 147.1
(-3)
A 		147.5
(-3)
A 		171.1
(-2.1)
A 		271.1
(1.4)
P 		283.2
(1.9)
A 		315.4
(3)
P 		168
B 220.9
(-1.5)
M 		173.9
(-3)
A 		204.1
(-2)
P 		213.5
(-1.7)
A 		357.4
(3)
P 		302.6
(1.2)
P 		184
C 127.9
(-3)
A 		142.4
(-2.6)
A 		192.3
(-1.1)
A 		248.6
(0.5)
P 		276.6
(1.4)
P 		332.4
(3)
P 		205
avr 165.3
(-2.6) 		154.6
(-3) 		189.2
(-1.7) 		244.4
(0.3) 		305.7
(2.6) 		316.8
(3) 		162
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 37
1 2 3 4 range
A 40.6
(0)
A 		31.5
(-3)
P 		49.6
(3)
A 		40.3
(-0.1)
A 		18
B 62.7
(3)
A 		34.6
(-2.1)
A 		29.8
(-3)
A 		40.8
(-1)
A 		33
C 26.1
(-3)
A 		34.7
(1.1)
A 		38.6
(3)
A 		35.2
(1.4)
A 		13
avr 43.1
(3) 		33.6
(-3) 		39.3
(0.6) 		38.8
(0.3) 		10

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 118
1 2 3 4 5 6 range
A 27.1
(-3)
A 		52.5
(-0.4)
A 		37.3
(-2)
A 		68.8
(1.3)
P 		85.4
(3)
A 		76.8
(2.1)
P 		58
B 76.8
(-1.3)
M 		57.9
(-2.5)
A 		49.6
(-3)
P 		56.5
(-2.6)
A 		145.2
(3)
P 		112.8
(1)
P 		96
C 47.4
(-2.7)
A 		43.5
(-3)
A 		48.9
(-2.5)
A 		50
(-2.4)
P 		89.5
(1.1)
P 		111.3
(3)
P 		68
avr 50.4
(-2.5) 		51.3
(-2.4) 		45.3
(-3) 		58.4
(-1.7) 		106.7
(3) 		100.3
(2.4) 		61
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176868_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19157
Sequence Type Exemplar sequence
Representative Public ID ZK616.I NCBI
Target Description ZK616.I /REP_DB=WormBase Gene ID /WP=CE26351 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF60949.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068403(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068403 NCBI Caenorhabditis elegans ZK616.2 (ZK616.2) mRNA, complete cds. 11/11 None
SNAP00000016316 ENSEMBL cdna:SNAP chromosome:CEL140:IV:5397911:5398531:1 9/11 None
GENEFINDER00000016326 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:5397059:5398531:1 10/11 None
ZK616.2 ENSEMBL cdna:known chromosome:CEL140:IV:5397854:5398531:1 gene:ZK616.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:5397859-5398537(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl ZK616.2
Entrez Gene 191359 Entrez gene
SwissProt Q9N4N1 EMBL-EBI
WormBase CE26351 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAF60948 Hypothetical protein ZK616.3 [Caenorhabditis elegans] ref|NP_500803.1| ZK616.3 [Caenorhabditis elegans] 8.0E-72
blast AAF60949 Hypothetical protein ZK616.2 [Caenorhabditis elegans] ref|NP_500804.1| ZK616.2 [Caenorhabditis elegans] 8.0E-53
blast AAF60949 Hypothetical protein ZK616.2 [Caenorhabditis elegans] ref|NP_500804.1| ZK616.2 [Caenorhabditis elegans] 6.0E-48
blast CAE72713 Hypothetical protein CBG19950 [Caenorhabditis briggsae] 1.0E-43
blast AAF60949 Hypothetical protein ZK616.2 [Caenorhabditis elegans] ref|NP_500804.1| ZK616.2 [Caenorhabditis elegans] 5.0E-42
blast CAE72713 Hypothetical protein CBG19950 [Caenorhabditis briggsae] 1.0E-39
blast CAE72713 Hypothetical protein CBG19950 [Caenorhabditis briggsae] 8.0E-35
blast AAF60948 Hypothetical protein ZK616.3 [Caenorhabditis elegans] ref|NP_500803.1| ZK616.3 [Caenorhabditis elegans] 4.0E-10
Protein Domains
Database ID Description E-Value
Pfam IPR010625 EMBL-EBI CHCH 1.3E-8
Pfam IPR010625 EMBL-EBI CHCH 0.17
Sequence
Target Sequence 
>C. ELEGANS:176868_AT
gagattttaaagttgggccgcgaagagtttttcaaatcggtaaaaatggaataccgacca
tattttgagccatatgagacacctgaatccgtgtatcccgacggcagcatcaacatcgac
tgcacctgcctccattctgctcttgctcatcgatgtggatatttgataagagatgcttta
gtatgcttcaacgcttcgaaaaccacaccacgcggcttggactgtgagaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGATTTTAAAGTTGGGCCGCGAAG 221 397 25 Antisense
GGCCGCGAAGAGTTTTTCAAATCGG 139 543 40 Antisense
GAATACCGACCATATTTTGAGCCAT 407 325 73 Antisense
GAGCCATATGAGACACCTGAATCCG 472 385 91 Antisense
TGAGACACCTGAATCCGTGTATCCC 243 569 99 Antisense
TGTATCCCGACGGCAGCATCAACAT 657 559 116 Antisense
GCAGCATCAACATCGACTGCACCTG 186 313 128 Antisense
CTGCTCTTGCTCATCGATGTGGATA 623 237 161 Antisense
GAGATGCTTTAGTATGCTTCAACGC 17 397 194 Antisense
TGCTTCAACGCTTCGAAAACCACAC 688 579 208 Antisense
CACCACGCGGCTTGGACTGTGAGAA 369 201 230 Antisense
Sequence Source Affymetrix Proprietary Database