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[176867_at]

Description Y5H2A.C /REP_DB=WormBase Gene ID /WP=CE26219 /CHR=5 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF59626.1
Cluster 6grp_total > 44      4grp_total > 11      6grp_cgc > 45      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 169
1 2 3 4 range
A 60.7
(-1.5)
P 		78.5
(-0.5)
A 		32.8
(-3)
A 		141.8
(3)
P 		109
B 51.5
(-3)
P 		91.8
(-1.4)
A 		58.2
(-2.7)
A 		198.7
(3)
P 		147
C 80.5
(-1.1)
P 		38.5
(-2.7)
A 		29.5
(-3)
A 		194
(3)
P 		165
avr 64.2
(-2) 		69.6
(-1.7) 		40.2
(-3) 		178.2
(3) 		138

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 91
1 2 3 4 5 6 range
A 75.1
(1.1)
A 		33.4
(-2.5)
A 		96.2
(3)
P 		28
(-3)
A 		58.2
(-0.3)
A 		93.4
(2.8)
M 		68
B 60.2
(0.9)
P 		73.4
(3)
P 		58.2
(0.6)
A 		35.5
(-3)
A 		40.9
(-2.1)
P 		39.9
(-2.3)
A 		38
C 61.5
(1.2)
P 		55
(0.7)
P 		85.4
(3)
P 		5.4
(-3)
A 		31.8
(-1)
A 		85.4
(3)
P 		80
avr 65.6
(1.5) 		53.9
(0.3) 		79.9
(3) 		23
(-3) 		43.6
(-0.8) 		72.9
(2.3) 		57
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 34
1 2 3 4 range
A 22.1
(3)
P 		17
(0.7)
A 		8.6
(-3)
A 		20.8
(2.4)
P 		14
B 16.4
(-2.4)
P 		22.7
(-1.1)
A 		13.5
(-3)
A 		42.4
(3)
P 		29
C 26.6
(1.4)
P 		11.1
(-2.8)
A 		10.3
(-3)
A 		32.7
(3)
P 		22
avr 21.7
(0.1) 		16.9
(-1.3) 		10.8
(-3) 		32
(3) 		21

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 28
1 2 3 4 5 6 range
A 13.8
(-0.4)
A 		11.9
(-1.2)
A 		20.9
(2.3)
P 		7.1
(-3)
A 		17.6
(1)
A 		22.7
(3)
M 		16
B 21
(1.6)
P 		24.4
(3)
P 		14.1
(-1.1)
A 		9.4
(-3)
A 		16.6
(-0.1)
P 		14.9
(-0.8)
A 		15
C 22.8
(1.7)
P 		16.8
(0.4)
P 		21.7
(1.5)
P 		1.1
(-3)
A 		10.3
(-1)
A 		28.6
(3)
P 		28
avr 19.2
(1.9) 		17.7
(1.4) 		18.9
(1.8) 		5.9
(-3) 		14.8
(0.3) 		22.1
(3) 		16
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176867_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19467
Sequence Type Exemplar sequence
Representative Public ID Y5H2A.C NCBI
Target Description Y5H2A.C /REP_DB=WormBase Gene ID /WP=CE26219 /CHR=5 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF59626.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071221(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071221 NCBI Caenorhabditis elegans Y5H2A.2 (Y5H2A.2) mRNA, complete cds. 11/11 None
SNAP00000022365 ENSEMBL cdna:SNAP chromosome:CEL140:V:2264832:2266875:-1 11/11 None
GENEFINDER00000022370 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:2264832:2266875:-1 11/11 None
Y5H2A.2 ENSEMBL cdna:known chromosome:CEL140:V:2264774:2266780:-1 gene:Y5H2A.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:2264773-2266780(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol nhr-275
Ensembl Y5H2A.2
Entrez Gene 189360 Entrez gene
SwissProt Q966A2 EMBL-EBI
WormBase CE26219 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6355 regulation of transcription, DNA-dependent inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5634 nucleus inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3700 transcription factor activity inferred from electronic annotation QuickGO AmiGO
3707 steroid hormone receptor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast NP_503622 Y5H2A.2 [Caenorhabditis elegans] gb|AAK71409.1| Hypothetical protein Y5H2A.2 [Caenorhabditis elegans] 0.0
blast CAE58144 Hypothetical protein CBG01233 [Caenorhabditis briggsae] 1.0E-105
blast CAE58144 Hypothetical protein CBG01233 [Caenorhabditis briggsae] 1.0E-100
blast AAF02172 Nuclear hormone receptor family protein 59 [Caenorhabditis elegans] ref|NP_503624.2| Nuclear Hormone Receptor family member (nhr-59) [Caenorhabditis elegans] gb|AAG15156.1| nuclear receptor NHR-59 [Caenorhabditis elegans] sp|Q9TXJ1|NHR59_CAEEL Nuclear hormone receptor family member nhr-59 2.0E-93
Protein Domains
Database ID Description E-Value
Pfam IPR001628 EMBL-EBI Zn-finger, C4-type steroid receptor 3.9E-10
Sequence
Target Sequence 
>C. ELEGANS:176867_AT
tggttgacccattttgacgagtttcaggaattgaatcaaaatattaagctagaattttta
aaaataatttggagcatatggggaaggctggagaagttggctcagaatgcacttttccat
cggagctataataataatgatacctcgaagcttttagtgggtgatgatgggattgtggat
aggaggaatgtgaaattggacattggatggctcacggattactcggtggagcaatttgca
tactatattgaaagccgcagagatggagttctgaacaagccgcttcaagccatgatcgac
ctgaacccaaccgacattgagctcaactacatgctggctcagctctccttttcctatgct
tcaaagcatttccctggtgaaggaaataaactggaaattgcggagcaccttcaacaactg
attgccgacgatttgcatgcctactatacagaagagctg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGGTTGACCCATTTTGACGAGTTTC 547 549 568 Antisense
GAATGCACTTTTCCATCGGAGCTAT 121 331 672 Antisense
GATGGCTCACGGATTACTCGGTGGA 534 405 773 Antisense
GTTCTGAACAAGCCGCTTCAAGCCA 183 443 835 Antisense
TTCAAGCCATGATCGACCTGAACCC 5 685 851 Antisense
ACCCAACCGACATTGAGCTCAACTA 253 81 872 Antisense
TCTCCTTTTCCTATGCTTCAAAGCA 678 609 911 Antisense
TGCTTCAAAGCATTTCCCTGGTGAA 632 579 924 Antisense
GCACCTTCAACAACTGATTGCCGAC 298 315 972 Antisense
GATTGCCGACGATTTGCATGCCTAC 419 433 987 Antisense
GCATGCCTACTATACAGAAGAGCTG 308 309 1002 Antisense
Sequence Source Affymetrix Proprietary Database