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[176864_at]

Description Y5H2A.B /REP_DB=WormBase Gene ID /WP=CE21312 /CHR=5 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF59625.1
Cluster 6grp_total > 83      4grp_total > 18      6grp_total_sub > Hat      6grp_rule_based > S4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 103
1 2 3 4 range
A 36.7
(-2.9)
A 		37.4
(-2.9)
A 		35.7
(-3)
A 		107.9
(3)
A 		72
B 27.5
(-0.4)
A 		56.5
(3)
A 		5.2
(-3)
A 		37.8
(0.8)
A 		51
C 22
(-3)
A 		39.8
(0.2)
A 		54.1
(2.8)
A 		55.1
(3)
A 		33
avr 28.7
(-3) 		44.6
(-0.5) 		31.7
(-2.5) 		66.9
(3) 		38

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 101
1 2 3 4 5 6 range
A 76.2
(0.4)
A 		29.9
(-3)
A 		49.4
(-1.6)
A 		87.4
(1.2)
A 		111.1
(3)
P 		63.5
(-0.5)
A 		81
B 46.1
(-3)
A 		51.6
(-2.3)
A 		50.8
(-2.4)
A 		94.2
(3)
A 		85.1
(1.9)
P 		73.3
(0.4)
A 		48
C 30.1
(-2.3)
A 		56.2
(-0.7)
A 		66.9
(-0.1)
A 		119
(3)
P 		99.8
(1.9)
P 		18.1
(-3)
A 		101
avr 50.8
(-2.5) 		45.9
(-3) 		55.7
(-1.9) 		100.2
(3) 		98.7
(2.8) 		51.6
(-2.4) 		54
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 18
1 2 3 4 range
A 13.4
(1.1)
A 		8.1
(-3)
A 		9.3
(-2.1)
A 		15.8
(3)
A 		8
B 8.8
(0.6)
A 		13.9
(3)
A 		1.2
(-3)
A 		8.1
(0.3)
A 		13
C 7.3
(-3)
A 		11.4
(-0.9)
A 		18.9
(3)
A 		9.3
(-2)
A 		12
avr 9.8
(-3) 		11.1
(3) 		9.8
(-3) 		11.1
(3) 		1

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 29
1 2 3 4 5 6 range
A 14
(-2.1)
A 		10.6
(-3)
A 		10.7
(-3)
A 		22.2
(0)
A 		33.5
(3)
P 		15.5
(-1.7)
A 		23
B 16
(-2)
A 		17.2
(-1.7)
A 		12.3
(-3)
A 		25
(0.4)
A 		34.6
(3)
P 		27.3
(1)
A 		22
C 11.2
(-1.8)
A 		17.2
(-0.4)
A 		17
(-0.5)
A 		23.9
(1.1)
P 		32.3
(3)
P 		6
(-3)
A 		26
avr 13.7
(-2.9) 		15
(-2.5) 		13.3
(-3) 		23.7
(0.1) 		33.5
(3) 		16.3
(-2.1) 		20
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176864_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19514
Sequence Type Exemplar sequence
Representative Public ID Y5H2A.B NCBI
Target Description Y5H2A.B /REP_DB=WormBase Gene ID /WP=CE21312 /CHR=5 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF59625.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071222(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071222 NCBI Caenorhabditis elegans Y5H2A.1 (Y5H2A.1) mRNA, complete cds. 11/11 None
SNAP00000022366 ENSEMBL cdna:SNAP chromosome:CEL140:V:2271003:2271663:-1 9/11 None
GENEFINDER00000022369 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:2271003:2271663:-1 11/11 None
Y5H2A.1 ENSEMBL cdna:known chromosome:CEL140:V:2271003:2271663:-1 gene:Y5H2A.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:2271002-2271663(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y5H2A.1
Entrez Gene 189359 Entrez gene
SwissProt Q9N4R3 EMBL-EBI
WormBase CE21312 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_503623 Y5H2A.1 [Caenorhabditis elegans] gb|AAF59625.1| Hypothetical protein Y5H2A.1 [Caenorhabditis elegans] 1.0E-80
blast NP_503623 Y5H2A.1 [Caenorhabditis elegans] gb|AAF59625.1| Hypothetical protein Y5H2A.1 [Caenorhabditis elegans] 1.0E-77
blast AAB65958 Hypothetical protein F41E6.8 [Caenorhabditis elegans] ref|NP_505217.1| F41E6.8 [Caenorhabditis elegans] 2.0E-67
blast AAB65958 Hypothetical protein F41E6.8 [Caenorhabditis elegans] ref|NP_505217.1| F41E6.8 [Caenorhabditis elegans] 4.0E-61
blast AAM15603 Hypothetical protein T13C5.2 [Caenorhabditis elegans] ref|NP_509036.2| T13C5.2 [Caenorhabditis elegans] 8.0E-40
blast AAM15603 Hypothetical protein T13C5.2 [Caenorhabditis elegans] ref|NP_509036.2| T13C5.2 [Caenorhabditis elegans] 1.0E-39
Protein Domains
Database ID Description E-Value
Pfam IPR005044 EMBL-EBI Caenorhabditis protein of unknown function DUF282 6.0E-50
Pfam IPR005044 EMBL-EBI Caenorhabditis protein of unknown function DUF282 3.7E-13
Pfam IPR005044 EMBL-EBI Caenorhabditis protein of unknown function DUF282 6.0E-50
Pfam IPR005044 EMBL-EBI Caenorhabditis protein of unknown function DUF282 3.7E-13
Sequence
Target Sequence 
>C. ELEGANS:176864_AT
aaatgtacacgtacctgcattccaacacaacaagttgacacaaacacaacaactacaact
gttgccacaacaacgactacagtgtccgaagctccagatccctgcacagtttgtcctaaa
acatatgacacaacctgccaaggatttggcatccctaatttgataagttggtgtcctaca
gccgaagaagtaggagtcacctatgtagttggtttactctcaattatcccactacttccg
tcggatacatgctcaacaaccattgtttgtccattaggaacaacagtatactacaatgtt
ctcggtacagaacttccaggtccatcacctattattgcgtggtgcagacaatcgggcaca
gacgtcggaacctggcacactggaattcctccgttggatg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAATGTACACGTACCTGCATTCCAA 44 121 52 Antisense
CGACTACAGTGTCCGAAGCTCCAGA 674 251 125 Antisense
GATCCCTGCACAGTTTGTCCTAAAA 276 417 148 Antisense
AACATATGACACAACCTGCCAAGGA 488 137 171 Antisense
GATAAGTTGGTGTCCTACAGCCGAA 296 421 213 Antisense
GTTGGTTTACTCTCAATTATCCCAC 41 435 259 Antisense
ATCCCACTACTTCCGTCGGATACAT 249 37 277 Antisense
TCGGTACAGAACTTCCAGGTCCATC 159 605 353 Antisense
GGTCCATCACCTATTATTGCGTGGT 421 501 370 Antisense
GACAATCGGGCACAGACGTCGGAAC 505 361 398 Antisense
CACACTGGAATTCCTCCGTTGGATG 636 193 427 Antisense
Sequence Source Affymetrix Proprietary Database