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[176861_at]

Description ZK616.A /REP_DB=WormBase Gene ID /WP=CE26343 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF60942.1
Cluster 6grp_total > 53      4grp_total > 25      6grp_cgc > 91      6grp_cgc_sub > 03      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 88
1 2 3 4 range
A 12.3
(-3)
A 		17.5
(-2.6)
A 		54.4
(0.2)
A 		91.6
(3)
A 		79
B 16.1
(2)
A 		10.4
(-0.3)
A 		18.7
(3)
A 		3.8
(-3)
A 		15
C 21.3
(-2.2)
A 		72.8
(3)
A 		12.9
(-3)
A 		53.7
(1.1)
A 		60
avr 16.6
(-3) 		33.6
(0.1) 		28.7
(-0.8) 		49.7
(3) 		33

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 41
1 2 3 4 5 6 range
A 14.5
(-1.3)
A 		3
(-3)
A 		44.4
(3)
A 		9.8
(-2)
A 		8.7
(-2.2)
A 		11
(-1.8)
A 		41
B 8.9
(-2.5)
A 		8.1
(-2.8)
A 		22.5
(3)
A 		7.6
(-3)
A 		16.4
(0.5)
A 		9.5
(-2.2)
A 		15
C 10
(1.2)
A 		4.4
(-3)
A 		12.4
(3)
A 		10.1
(1.3)
A 		7
(-1.1)
A 		11.8
(2.6)
A 		8
avr 11.1
(-1.3) 		5.2
(-3) 		26.4
(3) 		9.2
(-1.9) 		10.7
(-1.4) 		10.8
(-1.4) 		21
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 20
1 2 3 4 range
A 4.5
(-2.6)
A 		3.8
(-3)
A 		14.2
(3)
A 		13.4
(2.5)
A 		10
B 5.1
(3)
A 		2.6
(-0.5)
A 		4.3
(1.9)
A 		0.8
(-3)
A 		4
C 7
(-2.1)
A 		20.9
(3)
A 		4.5
(-3)
A 		9.1
(-1.3)
A 		16
avr 5.5
(-3) 		9.1
(3) 		7.7
(0.7) 		7.8
(0.8) 		4

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 9
1 2 3 4 5 6 range
A 2.7
(-1.9)
A 		1.1
(-3)
A 		9.7
(3)
A 		2.5
(-2)
A 		2.6
(-2)
A 		2.7
(-1.9)
A 		9
B 3.1
(-1.6)
A 		2.7
(-2.1)
A 		5.5
(1.5)
A 		2
(-3)
A 		6.7
(3)
A 		3.6
(-1)
A 		5
C 3.7
(2.5)
A 		1.3
(-3)
A 		3.2
(1.4)
A 		2
(-1.4)
A 		2.2
(-0.9)
A 		3.9
(3)
A 		3
avr 3.2
(-1) 		1.7
(-3) 		6.1
(3) 		2.2
(-2.3) 		3.8
(-0.1) 		3.4
(-0.7) 		4
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176861_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19354
Sequence Type Exemplar sequence
Representative Public ID ZK616.A NCBI
Target Description ZK616.A /REP_DB=WormBase Gene ID /WP=CE26343 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF60942.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001028444(11), NM_001028445(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001028444 NCBI Caenorhabditis elegans Enhancer of HAND mutation hnd-1 family member (ehn-3) (ehn-3) mRNA, complete cds. 11/11 None
NM_001028445 NCBI Caenorhabditis elegans Enhancer of HAND mutation hnd-1 family member (ehn-3) (ehn-3) mRNA, complete cds. 11/11 None
SNAP00000016322 ENSEMBL cdna:SNAP chromosome:CEL140:IV:5416893:5418041:-1 11/11 None
GENEFINDER00000016329 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:5416893:5418908:-1 9/11 None
ZK616.10b ENSEMBL cdna:known chromosome:CEL140:IV:5416893:5418041:-1 gene:ZK616.10 11/11 None
ZK616.10a ENSEMBL cdna:known chromosome:CEL140:IV:5416893:5419182:-1 gene:ZK616.10 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_001028446 8/11 Cross Hyb Matching Probes None
ZK616.10c 8/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
191363 3 2 NM_001028444 176861_at (11)
NM_001028445 176861_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:5416898-5418047(-) 100.0 100.0
Public Domain and Genome References
Ensembl ZK616.10
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAU87800 Enhancer of hand mutation hnd-1 protein 3, isoform a [Caenorhabditis elegans] ref|NP_001023615.1| Enhancer of HAND mutation hnd-1 family member (ehn-3) [Caenorhabditis elegans] 1.0E-144
blast AAF60942 Enhancer of hand mutation hnd-1 protein 3, isoform b [Caenorhabditis elegans] ref|NP_001023616.1| Enhancer of HAND mutation hnd-1 family member (ehn-3) [Caenorhabditis elegans] 1.0E-115
blast AAU87800 Enhancer of hand mutation hnd-1 protein 3, isoform a [Caenorhabditis elegans] ref|NP_001023615.1| Enhancer of HAND mutation hnd-1 family member (ehn-3) [Caenorhabditis elegans] 1.0E-115
Sequence
Target Sequence 
>C. ELEGANS:176861_AT
gaatgtcccatcacagcgtgcaattcaaagttcaacagcttcacgagtttgcagtttcat
gtagattcggagcatatcataagaggatcgtctccagctaaatgtaaatcttgcatcaaa
tggttcaacagttcccatcgtcttctgcttcattttcatactgcacacctggatcatacg
aaattcttctctttcaaacctgctccaattcttccaaaatccactacttcaatcttgaac
cccatcaagaatccagatgattttgatagaatctttgactttttcaaaactgaaatcgca
ccactctttccgcttcatagcactgtttcacaagccccattgccacagtgctctagatct
gtaaaatccgcaaaagagttgtctccaacaccatctacggaaatcgaaactcccgaagaa
gaagagttggatggtccagagagctggtactgcgattactgtaaaatcagattcgatgac
aaagtgatgtggtatttgcacagtggactccactcagatgatattccgtt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAATGTCCCATCACAGCGTGCAATT 636 331 58 Antisense
AGCTTCACGAGTTTGCAGTTTCATG 332 77 94 Antisense
TTTTCATACTGCACACCTGGATCAT 465 675 210 Antisense
TCTTCCAAAATCCACTACTTCAATC 58 617 267 Antisense
CAAAACTGAAATCGCACCACTCTTT 262 191 342 Antisense
TCTTTCCGCTTCATAGCACTGTTTC 690 615 362 Antisense
CCCATTGCCACAGTGCTCTAGATCT 327 257 393 Antisense
AAGAGTTGTCTCCAACACCATCTAC 428 153 431 Antisense
GAGAGCTGGTACTGCGATTACTGTA 107 389 496 Antisense
GGTATTTGCACAGTGGACTCCACTC 229 505 548 Antisense
GACTCCACTCAGATGATATTCCGTT 468 373 563 Antisense
Sequence Source Affymetrix Proprietary Database