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[176859_at]

Description ZK616.H /REP_DB=WormBase Gene ID /WP=CE26350 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF60948.1
Cluster 6grp_highest_10_10 > 56      6grp_high1_10_10 > 65      4grp_high_8_8 > 19      6grp_highest_nonorm > 91      6grp_total > 55      4grp_total > 19      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 126
1 2 3 4 range
A 142.1
(-1.5)
P 		122.7
(-2.5)
P 		114.1
(-3)
A 		222.9
(3)
P 		109
B 97
(-3)
A 		180.5
(3)
P 		126.6
(-0.9)
P 		138
(-0.1)
P 		84
C 144.6
(3)
P 		117
(-0.9)
P 		101.9
(-3)
P 		117.2
(-0.9)
P 		43
avr 127.9
(-1.2) 		140.1
(0.4) 		114.2
(-3) 		159.4
(3) 		45

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 180
1 2 3 4 5 6 range
A 160.3
(-1.9)
P 		189.4
(-0.8)
P 		296.2
(3)
P 		174.8
(-1.4)
P 		128.9
(-3)
P 		224
(0.4)
P 		167
B 168.3
(-1.4)
P 		164.3
(-1.5)
P 		283.7
(3)
P 		125.6
(-3)
P 		203.4
(-0)
P 		218.9
(0.5)
P 		158
C 155
(-2.2)
P 		217.2
(-0.1)
P 		132.8
(-3)
P 		182.5
(-1.3)
P 		183.7
(-1.2)
P 		305.6
(3)
P 		173
avr 161.2
(-3) 		190.3
(-1) 		237.6
(2.2) 		161
(-3) 		172
(-2.3) 		249.5
(3) 		89
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 32
1 2 3 4 range
A 51.7
(3)
P 		26.6
(-3)
P 		29.8
(-2.2)
A 		32.6
(-1.6)
P 		25
B 30.9
(-2.4)
A 		44.6
(3)
P 		29.3
(-3)
P 		29.4
(-3)
P 		15
C 47.9
(3)
P 		33.6
(-0.1)
P 		35.5
(0.4)
P 		19.8
(-3)
P 		28
avr 43.5
(3) 		34.9
(-0.2) 		31.5
(-1.4) 		27.3
(-3) 		16

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 73
1 2 3 4 5 6 range
A 29.6
(-3)
P 		67.4
(3)
P 		64.5
(2.5)
P 		44.3
(-0.7)
P 		38.9
(-1.5)
P 		54.5
(1)
P 		38
B 58.5
(0.1)
P 		54.7
(-0.4)
P 		68.9
(1.3)
P 		33.3
(-3)
P 		82.7
(3)
P 		81.6
(2.9)
P 		49
C 57.5
(-0.9)
P 		66.3
(-0.2)
P 		33.8
(-3)
P 		36.7
(-2.7)
P 		59.4
(-0.8)
P 		102.3
(3)
P 		69
avr 48.5
(-1.5) 		62.8
(0.6) 		55.7
(-0.4) 		38.1
(-3) 		60.3
(0.2) 		79.5
(3) 		41
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176859_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19326
Sequence Type Exemplar sequence
Representative Public ID ZK616.H NCBI
Target Description ZK616.H /REP_DB=WormBase Gene ID /WP=CE26350 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF60948.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068402(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068402 NCBI Caenorhabditis elegans ZK616.3 (ZK616.3) mRNA, complete cds. 11/11 None
SNAP00000016315 ENSEMBL cdna:SNAP chromosome:CEL140:IV:5397086:5397665:1 11/11 None
GENEFINDER00000016326 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:5397059:5398531:1 11/11 None
ZK616.3 ENSEMBL cdna:known chromosome:CEL140:IV:5397086:5397665:1 gene:ZK616.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:5397091-5397671(+) 98.41 100.0
Public Domain and Genome References
Gene Title
Ensembl ZK616.3
Entrez Gene 191360 Entrez gene
SwissProt Q9N4N2 EMBL-EBI
WormBase CE26350 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAF60948 Hypothetical protein ZK616.3 [Caenorhabditis elegans] ref|NP_500803.1| ZK616.3 [Caenorhabditis elegans] 2.0E-72
blast AAF60948 Hypothetical protein ZK616.3 [Caenorhabditis elegans] ref|NP_500803.1| ZK616.3 [Caenorhabditis elegans] 8.0E-72
blast AAF60949 Hypothetical protein ZK616.2 [Caenorhabditis elegans] ref|NP_500804.1| ZK616.2 [Caenorhabditis elegans] 6.0E-48
blast CAE72713 Hypothetical protein CBG19950 [Caenorhabditis briggsae] 1.0E-39
blast XP_576980 PREDICTED: similar to CHCHD4 protein [Rattus norvegicus] 5.0E-11
blast AAF60949 Hypothetical protein ZK616.2 [Caenorhabditis elegans] ref|NP_500804.1| ZK616.2 [Caenorhabditis elegans] 3.0E-10
Protein Domains
Database ID Description E-Value
Pfam IPR010625 EMBL-EBI CHCH 1.3E-8
Pfam IPR010625 EMBL-EBI CHCH 1.3E-8
Pfam IPR010625 EMBL-EBI CHCH 0.17
Sequence
Target Sequence 
>C. ELEGANS:176859_AT
atgcaatgccacgtcaaagatgaggtactgtacattggaaaaaaagaattcgaagagcca
ataaaatcggaatgggttctagaaatgcagaatacagaaaaatacaaaacaattggacca
acgtttccagatggaacaattaattggcagtgtccatgtatggcaggtggatctctggta
gcacataggtgcggcaacttcttccgagagctgtacatttgtatgaaaagcgacgacacg
agagatccatcggaaaaatgtccaaatcaatttgtaaattgggccgcctgtatgcagaat
atgagtgatgaacgacgagaacaaatgcggaaggcgatgactgtggat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGCAATGCCACGTCAAAGATGAGG 355 43 13 Antisense
ACAATTGGACCAACGTTTCCAGATG 599 111 121 Antisense
GGCAGTGTCCATGTATGGCAGGTGG 512 535 158 Antisense
GGCAGGTGGATCTCTGGTAGCACAT 648 533 174 Antisense
GCACATAGGTGCGGCAACTTCTTCC 237 317 193 Antisense
GCAACTTCTTCCGAGAGCTGTACAT 630 319 206 Antisense
CTTCCGAGAGCTGTACATTTGTATG 644 217 213 Antisense
GCGACGACACGAGAGATCCATCGGA 565 289 242 Antisense
GTAAATTGGGCCGCCTGTATGCAGA 73 461 286 Antisense
GGGCCGCCTGTATGCAGAATATGAG 361 493 293 Antisense
AATGCGGAAGGCGATGACTGTGGAT 445 165 336 Antisense
Sequence Source Affymetrix Proprietary Database