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[176858_at]

Description C25A6.B /REP_DB=WormBase Gene ID /WP=CE20524 /CHR=5 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF35957.1
Cluster 6grp_total > 01      4grp_total > 56      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 19
1 2 3 4 range
A 8.4
(-1)
A 		2.8
(-2.8)
A 		20.8
(3)
A 		2.2
(-3)
A 		19
B 8.5
(3)
A 		1.6
(-3)
A 		4.8
(-0.2)
A 		4.4
(-0.6)
A 		7
C 5
(-2)
A 		4.1
(-2.6)
A 		13.4
(3)
A 		3.4
(-3)
A 		10
avr 7.3
(-0.4) 		2.8
(-3) 		13
(3) 		3.3
(-2.7) 		10

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 29
1 2 3 4 5 6 range
A 20.5
(1.2)
A 		29
(3)
A 		5.7
(-2)
A 		6.3
(-1.9)
A 		2
(-2.8)
A 		1.3
(-3)
A 		28
B 1.6
(-3)
A 		6.4
(-0.7)
A 		3.2
(-2.2)
A 		3.3
(-2.2)
A 		14.2
(3)
A 		12.1
(2)
A 		13
C 2.1
(0.6)
A 		2.8
(2.1)
A 		3.2
(3)
A 		0.5
(-3)
A 		0.6
(-2.8)
A 		2.5
(1.4)
A 		3
avr 8.1
(0) 		12.7
(3) 		4
(-2.6) 		3.4
(-3) 		5.6
(-1.6) 		5.3
(-1.8) 		9
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 5
1 2 3 4 range
A 3.1
(0.3)
A 		0.6
(-2.6)
A 		5.4
(3)
A 		0.3
(-3)
A 		5
B 2.7
(3)
A 		0.4
(-3)
A 		1.1
(-1.2)
A 		0.9
(-1.7)
A 		2
C 1.6
(-1.5)
A 		1.2
(-2.1)
A 		4.7
(3)
A 		0.6
(-3)
A 		4
avr 2.5
(0.7) 		0.7
(-2.8) 		3.7
(3) 		0.6
(-3) 		3

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 10
1 2 3 4 5 6 range
A 3.8
(-0.9)
A 		10.3
(3)
A 		1.2
(-2.5)
A 		1.6
(-2.2)
A 		0.6
(-2.8)
A 		0.3
(-3)
A 		10
B 0.6
(-3)
A 		2.1
(-1.3)
A 		0.8
(-2.8)
A 		0.9
(-2.7)
A 		5.8
(3)
A 		4.5
(1.5)
A 		5
C 0.8
(2.3)
A 		0.9
(3)
A 		0.8
(2.3)
A 		0.1
(-3)
A 		0.2
(-2.3)
A 		0.8
(2.3)
A 		1
avr 1.7
(-1.6) 		4.4
(3) 		0.9
(-3) 		0.9
(-3) 		2.2
(-0.8) 		1.9
(-1.3) 		4
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176858_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19409
Sequence Type Exemplar sequence
Representative Public ID C25A6.B NCBI
Target Description C25A6.B /REP_DB=WormBase Gene ID /WP=CE20524 /CHR=5 /FEA=Sanger Annotation /DEF=(ST.LOUIS) protein_id:AAF35957.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001028504(11), NM_001028503(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001028504 NCBI Caenorhabditis elegans C25A6.1b (C25A6.1) mRNA, complete cds. 11/11 None
NM_001028503 NCBI Caenorhabditis elegans C25A6.1a (C25A6.1) mRNA, complete cds. 10/11 None
C25A6.1a ENSEMBL cdna:known chromosome:CEL140:V:5408340:5409773:1 gene:C25A6.1 10/11 None
C25A6.1b ENSEMBL cdna:known chromosome:CEL140:V:5408340:5409862:1 gene:C25A6.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:5408339-5409773(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C25A6.1
Entrez Gene 178923 Entrez gene
WormBase CE38785 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_001023674 C25A6.1a [Caenorhabditis elegans] 1.0E-145
blast AAY86224 Hypothetical protein C25A6.1 [Caenorhabditis elegans] 1.0E-139
blast CAB62794 Hypothetical protein C47A4.3 [Caenorhabditis elegans] ref|NP_502650.1| C47A4.3 [Caenorhabditis elegans] 1.0E-124
blast NP_001023675 C25A6.1b [Caenorhabditis elegans] 3.0E-89
blast AAY86224 Hypothetical protein C25A6.1 [Caenorhabditis elegans] 1.0E-76
blast NP_001023674 C25A6.1a [Caenorhabditis elegans] 1.0E-76
Protein Domains
Database ID Description E-Value
Pfam IPR004843 EMBL-EBI Metallo-phosphoesterase 1.6E-41
Pfam IPR004843 EMBL-EBI Metallo-phosphoesterase 5.2E-24
Sequence
Target Sequence 
>C. ELEGANS:176858_AT
aagctactttccgaagtgatcacggaaggacgagttctaaagcttctcgatttggcactg
gatgtatttaaaagacagaaatcaatggttgaaatgaatgctccgataaaggtgtgcggt
gacatccatggacaattcccggatcttcttcgtctcttccatcgtggtggatggccgccg
actgcgaattatttattccttggtgactatgtggaccgtggaagatttagcattgaaacc
attgttcttcttctcgcttataaggtgaaattcccgggcaatttattcttgttgcgtgga
aaccatgagtgcgagtttgtcaacaaggtatatggattctatgaagagtgtcagaaacgc
tatcaaagtgttcgcatgttcacggctttccaggaaaac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAGCTACTTTCCGAAGTGATCACGG 162 151 73 Antisense
GGAAGGACGAGTTCTAAAGCTTCTC 698 529 96 Antisense
AATGGTTGAAATGAATGCTCCGATA 455 163 156 Antisense
TGCTCCGATAAAGGTGTGCGGTGAC 101 581 171 Antisense
GAATTATTTATTCCTTGGTGACTAT 635 323 258 Antisense
ATTCCCGGGCAATTTATTCTTGTTG 632 5 342 Antisense
CAATTTATTCTTGTTGCGTGGAAAC 55 181 351 Antisense
GAGTGCGAGTTTGTCAACAAGGTAT 462 399 379 Antisense
TGAAGAGTGTCAGAAACGCTATCAA 10 575 414 Antisense
AAAGTGTTCGCATGTTCACGGCTTT 339 121 437 Antisense
CATGTTCACGGCTTTCCAGGAAAAC 153 211 447 Antisense
Sequence Source Affymetrix Proprietary Database